A RATIOMETRIC FLUORESCENCE METHOD TO MEASURE OXYGEN
    1.
    发明申请
    A RATIOMETRIC FLUORESCENCE METHOD TO MEASURE OXYGEN 审中-公开
    测量氧气的比例荧光法

    公开(公告)号:WO1995008107A1

    公开(公告)日:1995-03-23

    申请号:PCT/US1994010139

    申请日:1994-09-07

    Abstract: A ratiometric method for using optical sensors (11) is provided to measure dissolved oxygen in a fluid sample. The sensor (11) is formulated so as to contain an oxygen permeable membrane (16) of a cured perfluorinated urethane polymer and, incorporated therein, an oxygen-sensitive indicator component and a reference dye component. The emission spectrum from the indicator component is calibrated by comparison with that of the reference dye, which is substantially less sensitive to oxygen-quenching and which has a photodecomposition rate similar to the indicator component. Methods for making and using the membrane and sensor (11) are provided as well.

    Abstract translation: 提供了使用光学传感器(11)的比例计方法来测量流体样品中的溶解氧。 将传感器(11)配制为包含固化的全氟化氨基甲酸酯聚合物的透氧膜(16),并且其中并入氧敏指示剂组分和参考染料组分。 通过与参考染料的比较来校准来自指示剂组分的发射光谱,其对氧气淬灭基本上较不敏感,并且具有与指示剂组分相似的光分解速率。 还提供了制造和使用膜和传感器(11)的方法。

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