公开(公告)号：EP0411907A2

公开(公告)日：1991-02-06

申请号：EP90308424.2

申请日：1990-07-31

Applicant: ORTHO DIAGNOSTIC SYSTEMS INC.

Inventor： Schutt, Ernest G. ,  Greenwood, David L. ,  Utberg, Karin

IPC: G01N21/17 ,  G01N33/53

CPC classification number: G01N21/552 ,  G01J3/0216 ,  G01J3/0243 ,  G01J3/0297 ,  G01N21/21 ,  G01N21/47 ,  G01N33/553 ,  G01N33/585 ,  Y10S436/807

Abstract: A new immunoassay system is provided for the detection of ligands or ligand binding partners in solution in a heterogeneous format. The invention relies upon the detection of back scattered light from an evanescent wave disturbed by the presence of a colloidal gold label brought to the interface by an immunological reaction. The evanescent wave existing at the interface in turn is the result of a totally internally reflected incident light wave. Placement of the detector at a back angle above the critical angle insures a superior signal-to-noise ratio. Apparatus and methods for scanning, detecting and manipulating light including a scattered total internal reflectance immunoassay system are provided.

Abstract translation: 提供了一种新的免疫测定系统，用于以异质形式检测溶液中的配体或配体结合配偶体。 本发明依赖于通过由免疫反应引入界面的胶体金标记的存在而扰乱的ev逝波的背散射光的检测。 接口中存在的ev逝波依次是全反射入射光波的结果。 在高于临界角的后角处放置检测器确保了优异的信噪比。 提供了用于扫描，检测和操纵包括散射的全内反射免疫测定系统的光的装置和方法。

公开(公告)号：EP0411907B1

公开(公告)日：1997-06-11

申请号：EP90308424.2

申请日：1990-07-31

Applicant: ORTHO DIAGNOSTIC SYSTEMS INC.

Inventor： Schutt, Ernest G. ,  Greenwood, David L. ,  Utberg, Karin

IPC: G01N21/17 ,  G01N33/53

CPC classification number: G01N21/552 ,  G01J3/0216 ,  G01J3/0243 ,  G01J3/0297 ,  G01N21/21 ,  G01N21/47 ,  G01N33/553 ,  G01N33/585 ,  Y10S436/807

公开(公告)号：EP0411907A3

公开(公告)日：1991-07-24

申请号：EP90308424.2

申请日：1990-07-31

Applicant: ORTHO DIAGNOSTIC SYSTEMS INC.

Inventor： Schutt, Ernest G. ,  Greenwood, David L. ,  Utberg, Karin

IPC: G01N21/17 ,  G01N33/53

CPC classification number: G01N21/552 ,  G01J3/0216 ,  G01J3/0243 ,  G01J3/0297 ,  G01N21/21 ,  G01N21/47 ,  G01N33/553 ,  G01N33/585 ,  Y10S436/807

Abstract: A new immunoassay system is provided for the detection of ligands or ligand binding partners in solution in a heterogeneous format. The invention relies upon the detection of back scattered light from an evanescent wave disturbed by the presence of a colloidal gold label brought to the interface by an immunological reaction. The evanescent wave existing at the interface in turn is the result of a totally internally reflected incident light wave. Placement of the detector at a back angle above the critical angle insures a superior signal-to-noise ratio. Apparatus and methods for scanning, detecting and manipulating light including a scattered total internal reflectance immunoassay system are provided.