公开(公告)号：WO2014191850A1

公开(公告)日：2014-12-04

申请号：PCT/IB2014/060793

申请日：2014-04-17

Applicant: OPTOELETTRONICA ITALIA S.R.L

Inventor： MAGLIONE, Alfredo ,  RESS, Cristina

IPC: G01N21/64

CPC classification number: C12Q1/6876 ,  C12Q1/6825 ,  C12Q2600/178 ,  G01N21/63 ,  G01N21/6428 ,  G01N21/645 ,  G01N21/77 ,  G01N2201/022 ,  G01N2201/062 ,  G01N2201/0633

Abstract: It's disclosed a kit for detecting a micro-RNA of interest in at least one sample (C) extracted from a body fluid, comprising: at least one device (2) including a housing casing (2a) in which at least one housing seat (2b) is obtained for said at least one sample (C), and at least one opening (2c) through which said housing seat (2b) is accessible from the outside; at least one container means (3) for said at least one sample (C), said at least one container means (3) being insertable/disconnectable in/from said housing seat (2b) through said at least one opening (2c); at least one optical excitation group (5), housed in said housing casing (2a), designed to emit at least one excitation light radiation (λ, λ1 ) towards said at least one housing seat (2b); at least one detection group (6), designed to detect at least one emission light radiation (λ2), that can be generated, in use, by said at least one sample (C), said at least one sample (C) being optically excitable by said at least one excitation light radiation (λ, λ1) emitted by said at least one optical excitation group (5), said at least one detection group (6) being designed to supply at least one electric output signal (SO- signal output) correlated with the quantity, in said at least one sample (C), of said micro-RNA of interest; at least one processing unit (7) designed to receive and process said at least one electric signal (SO) and to output an index correlated with the quantity of said micro-RNA of interest in said at least one sample (C); said at least one container means (3) being made of a material permeable to said at least one excitation light radiation (λ, λ1) and to said at least one emission light radiation (A2); said at least one group (6) for detecting said emission light radiation (A2) comprises at least one sensor means (6a) of silicon photomultiplier type.

Abstract translation: 公开了用于检测从体液中提取的至少一个样品（C）中感兴趣的微RNA的试剂盒，其包括：至少一个装置（2），其包括壳体壳体（2a），其中至少一个壳体座 获得所述至少一个样品（C）的至少一个开口（2c），以及至少一个开口（2c），所述壳体座（2b）可以从外部进入; 用于所述至少一个样品（C）的至少一个容器装置（3），所述至少一个容器装置（3）可通过所述至少一个开口（2c）从所述壳体座（2b）插入/断开; 容纳在所述壳体壳体（2a）中的至少一个光学激发组（5）被设计成朝向所述至少一个壳体座（2b）发射至少一个激发光辐射（λ，λ1）; 至少一个检测组（6），被设计成检测在所述至少一个样品（C）中可以在使用中产生的至少一个发射光辐射（λ2），所述至少一个样品（C）是光学的 由所述至少一个光学激发组（5）发射的所述至少一个激发光辐射（λ，λ1）激发，所述至少一个检测组（6）被设计成提供至少一个电输出信号（SO-信号 输出）与所述至少一个样品（C）中所述感兴趣的微RNA的量相关; 至少一个处理单元（7）被设计为接收和处理所述至少一个电信号（SO）并输出与所述至少一个样品（C）中的所述感兴趣的微RNA的量相关的指数; 所述至少一个容器装置（3）由可透过所述至少一个激发光辐射（λ，λ1）和所述至少一个发射光辐射（A2）的材料制成; 所述至少一个用于检测所述发射光辐射的组（6）包括至少一个硅光电倍增器类型的传感器装置（6a）。

公开(公告)号：EP4279904A1

公开(公告)日：2023-11-22

申请号：EP23173727.1

申请日：2023-05-16

Applicant: Optoelettronica Italia S.R.L.

Inventor： MAGLIONE, Alfredo ,  DETASSIS, Simone ,  RESS, Cristina ,  LUTTERI, Marco

IPC: G01N21/64 ,  C12Q1/6818 ,  C12Q1/6825

Abstract: Device for detecting biomolecules, comprising a container (2) intended to be filled with a mixture comprising a biomolecule (30) to be detected, a reagent (4) bondable to the biomolecule (30) and susceptible of emitting a first light radiation (λ 1 ) in fluorescence or chemiluminescence, and a plurality of magnetic beads (41) chemically bondable to the biomolecule (30). The device comprises a first detector (71) for detecting the first light radiation (λ 1 ) for detecting the biomolecule (30) concentration (C), a first emitter (61) for emitting a second light radiation (λ 2 ) for irradiating the magnetic beads (41), and a second detector (72) for detecting a third light radiation (λ 3 ) consequently transmitted, reflected or emitted by the magnetic beads (41) for detecting the quantity of magnetic beads (41). The device also comprises a logic control unit (8) configured for calculating a correct concentration (C) value of biomolecule (30) on the basis of the above measurements of the light radiations (λ 1 , λ 3 ).

公开(公告)号：EP3004846A1

公开(公告)日：2016-04-13

申请号：EP14723887.7

申请日：2014-04-17

Applicant: Optoelettronica Italia S.R.L.

Inventor： MAGLIONE, Alfredo ,  RESS, Cristina

IPC: G01N21/64

CPC classification number: C12Q1/6876 ,  C12Q1/6825 ,  C12Q2600/178 ,  G01N21/63 ,  G01N21/6428 ,  G01N21/645 ,  G01N21/77 ,  G01N2201/022 ,  G01N2201/062 ,  G01N2201/0633

Abstract: It's disclosed a kit for detecting a micro-RNA of interest in at least one sample (C) extracted from a body fluid, comprising: at least one device (2) including a housing casing (2a) in which at least one housing seat (2b) is obtained for said at least one sample (C), and at least one opening (2c) through which said housing seat (2b) is accessible from the outside; at least one container means (3) for said at least one sample (C), said at least one container means (3) being insertable/disconnectable in/from said housing seat (2b) through said at least one opening (2c); at least one optical excitation group (5), housed in said housing casing (2a), designed to emit at least one excitation light radiation (λ, λ1 ) towards said at least one housing seat (2b); at least one detection group (6), designed to detect at least one emission light radiation (λ2), that can be generated, in use, by said at least one sample (C), said at least one sample (C) being optically excitable by said at least one excitation light radiation (λ, λ1) emitted by said at least one optical excitation group (5), said at least one detection group (6) being designed to supply at least one electric output signal (SO- signal output) correlated with the quantity, in said at least one sample (C), of said micro-RNA of interest; at least one processing unit (7) designed to receive and process said at least one electric signal (SO) and to output an index correlated with the quantity of said micro-RNA of interest in said at least one sample (C); said at least one container means (3) being made of a material permeable to said at least one excitation light radiation (λ, λ1) and to said at least one emission light radiation (A2); said at least one group (6) for detecting said emission light radiation (A2) comprises at least one sensor means (6a) of silicon photomultiplier type.