公开(公告)号：US20230410309A1

公开(公告)日：2023-12-21

申请号：US18198501

申请日：2023-05-17

Applicant: Overture Life, Inc.

Inventor： Adrián ALVAREZ FERNANDEZ ,  Santiago MUNNE ,  Luis MOLLINEDO HERRERA ,  Gloria CALDERON OYA ,  Nuno Luis COSTA BORGES ,  Jesús RAMOS MEMBRIVE ,  Sergi MAS SABATES

IPC: G06T7/00 ,  G06T7/30 ,  G06V20/69 ,  G06V20/70 ,  G06V10/82 ,  G06T7/70 ,  G06T7/20 ,  G06V10/774 ,  G16H30/40 ,  C12N15/89

CPC classification number: G06T7/0014 ,  G06T2207/30024 ,  G06V20/698 ,  G06V20/70 ,  G06V10/82 ,  G06T7/70 ,  G06T7/20 ,  G06V10/774 ,  G16H30/40 ,  C12N15/89 ,  G06T2207/30044 ,  G06T2207/20084 ,  G06T2207/10056 ,  G06T2207/20036 ,  G06V2201/034 ,  G06T2207/20081 ,  G06T7/30

Abstract: Provided herein are methods, computer programs, and systems for automated microinjection, for example, automated Intracytoplasmic Sperm Injection (ICSI). Methods described herein include creating, by a processing unit, a first dataset of an oocyte and a holding device and a second dataset of an injection pipette; detecting the oocyte and the holding device in the first dataset and the injection pipette in the second dataset; selecting the image of the first dataset and of the second dataset where an equatorial plane of the oocyte/holding device and of the injection pipette has an improved focusing parameter; selecting images of the first and second datasets and labeling the pixels associated with the oocyte and to the injection pipette; detecting a tip of the injection pipette; detecting different morphological structures of the oocyte using artificial intelligence computer vision algorithms on the first dataset; creating an injection trajectory for the injection pipette to perform the ICSI using the detected morphological structures; detecting when the oocyte is rupturing and when the spermatozoa has been released from the injection pipette into the cytoplasm of oocyte.