公开(公告)号：AU2014202286B2

公开(公告)日：2015-01-29

申请号：AU2014202286

申请日：2014-04-28

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： MCCAFFREY NATHANIEL JOSEPH ,  TURNER STEPHEN ,  SAXENA RAVI ,  HELGESEN SCOTT EDWARD

IPC: G01N21/00

Abstract: An analytical assembly within a unified device structure for integration into an analytical system. The analytical assembly is scalable and includes a plurality of analytical devices, each of which includes a reaction cell, an optical sensor, and at least one optical element positioned in optical communication with both the reaction cell and the sensor and which delivers optical signals from the cell to the sensor. Additional elements are optionally integrated into the analytical assembly. Methods for forming and operating the analytical system are also disclosed.

公开(公告)号：AU2011217862B9

公开(公告)日：2014-07-10

申请号：AU2011217862

申请日：2011-02-18

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： MCCAFFREY NATHANIEL JOSEPH ,  TURNER STEPHEN ,  SAXENA RAVI ,  HELGESEN SCOTT EDWARD

Abstract: A sequencing chip and a corresponding method for distinguishing fluorescently labelled species at a single molecule level, the chip comprising:a plurality of analytical devices, each analytical device having a reaction cell, an optical element and a detection element, wherein a sample is disposed at the reaction cell, and the optical element is in optical communication with the reaction cell and the detection element;a plurality of sources emitting at different wavelengths at different times; andone or more integrated waveguides in optical communication with the reaction cells to deliver a pulsed illumination to the reaction cells, wherein the pulsed illumination comprises two or more pulses at different wavelengths and different times, and the different wavelengths correspond to different excitation spectra of a plurality of fluorophores;wherein for each analytical device in the plurality of analytical devices:the pulsed illumination produces excitation events from the sample at the reaction cell, each excitation event producing an emitted signal;the optical element delivers the emitted signals to the detection element; andthe detection element detects the emitted signals by correlating the timing of the emitted signals detected at the detection element with the timing of the excitation events, thereby identifying whether any fluorophore in the plurality of fluorophores is present or absent in the reaction cell..

公开(公告)号：CA2790393C

公开(公告)日：2019-03-12

申请号：CA2790393

申请日：2011-02-18

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： MCCAFFREY NATHANIEL JOSEPH ,  TURNER STEPHEN ,  SAXENA RAVI ,  HELGESEN SCOTT EDWARD

IPC: G01N21/64 ,  B82Y15/00 ,  G01N21/01 ,  G01N21/11 ,  G01N21/63 ,  G08C23/04

Abstract: An analytical assembly within a unified device structure for integration into an analytical system. The analytical assembly is scalable and includes a plurality of analytical devices, each of which includes a reaction cell, an optical sensor, and at least one optical element positioned in optical communication with both the reaction cell and the sensor and which delivers optical signals from the cell to the sensor. Additional elements are optionally integrated into the analytical assembly. Methods for forming and operating the analytical system are also disclosed.