公开(公告)号：AU2007260707A1

公开(公告)日：2007-12-21

申请号：AU2007260707

申请日：2007-06-15

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： SHEN GENE ,  PELUSO PAUL ,  LYLE JOHN

IPC: C12Q1/68

Abstract: Controlled initiation of primer extension in determination of nucleic acid sequence information by incorporation of nucleotides or nucleotide analogs. Preferred aspects include photo-initiated extension through the use of photo-cleavable blocking, groups on termini of primer sequences followed by non-terminating primer extension using nucleotides or nucleotide analogs that are not extension terminators.

公开(公告)号：AU2009212165A1

公开(公告)日：2009-08-13

申请号：AU2009212165

申请日：2009-02-06

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： SHEN GENE ,  KONG XIANGXU ,  EMIG ROBIN ,  OTTO GEOFFREY

IPC: C07H21/00

公开(公告)号：EP3030683A4

公开(公告)日：2017-05-03

申请号：EP14834734

申请日：2014-08-05

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： SEBO LUBOMIR ,  HANES JEREMIAH ,  SHEN GENE ,  BROGLEY LOUIS ,  YUE STEPHEN ,  ZHENG FRANK ,  LAPIN YURI ,  LYLE JOHN ,  OSUNA HONEY ,  FEDOROV ANDREI

IPC: C12Q1/68 ,  C07F9/6558 ,  C07F9/6561 ,  C07H19/10 ,  G01N21/64 ,  G01N23/223

CPC classification number: C07H19/10 ,  C07F9/65586 ,  C07F9/65616

Abstract: Protected fluorescent reagent compounds and their methods of synthesis are provided. The compounds are useful in various fluorescence-based analytical methods, including the analysis of highly multiplexed optical reactions in large numbers at high densities, such as single molecule real time nucleic acid sequencing reactions. The compounds contain fluorescent dye elements, that allow the compounds to be detected with high sensitivity at desirable wavelengths, binding elements, that allow the compounds to be recognized specifically by target biomolecules, and protective shield elements, that decrease undesirable contacts between the fluorescent dye elements and the bound target biomolecules and that therefore decrease photodamage of the bound target biomolecules by the fluorescent dye elements.

Abstract translation: 提供受保护的荧光试剂化合物及其合成方法。 这些化合物可用于各种基于荧光的分析方法，包括高密度分析大量高度多重光学反应，如单分子实时核酸测序反应。 这些化合物含有荧光染料元素，可以在理想的波长下以高灵敏度检测化合物，允许化合物特异性地被目标生物分子识别的结合元件和保护性屏蔽元​​件，其减少荧光染料元素之间的不希望的接触 和结合的目标生物分子，并因此减少了荧光染料元件对结合的目标生物分子的光损伤。