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    Sequencing by cognate sampling
    3.
    发明专利
    Sequencing by cognate sampling 未知

    公开(公告)号：AU2009288696A1

    公开(公告)日：2010-03-11

    申请号：AU2009288696

    申请日：2009-09-04

    Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

    Inventor： PATEL PRANAV ,  KORLACH JONAS ,  BIBILLO ARKADIUSZ ,  BJORNSON KEITH ,  HANES JEREMIAH

    IPC: C12Q1/68 ,  C12N15/11 ,  C12Q1/25

    Abstract: Provided are methods for enhanced sequencing of nucleic acid templates. Also provided are reaction conditions that increase branching fractions during polymerization reactions. Also provided are compositions comprising modified recombinant polymerases that exhibit branching fractions that are higher than the branching fractions of the polymerases from which they were derived. Provided are compositions comprising modified recombinant polymerases that exhibit delayed translocation relative to the polymerases from which they were derived. Also provided are compositions comprising modified recombinant polymerases that exhibit increased nucleotide or nucleotide analog residence time at an active site of the polymerase. Provided are methods for generating polymerases with the aforementioned phenotypes and methods of using such polymerases to sequence a DNA template or make a DNA. Also provided are methods and nucleic acid sequencing systems for determining which labeled nucleotide is incorporated at a site during a template-dependent polymerization reaction.

    REAL-TIME ELECTRONIC SEQUENCING
    5.
    发明公开
    REAL-TIME ELECTRONIC SEQUENCING 有权
    电子实时序列

    公开(公告)号：EP2994544A4

    公开(公告)日：2017-01-25

    申请号：EP14794438

    申请日：2014-05-05

    Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

    Inventor： TURNER STEPHEN ,  HANES JEREMIAH ,  BJORNSON KEITH

    IPC: C12Q1/68

    CPC classification number: C12Q1/6874 ,  C12Q1/6823 ,  G01N27/227 ,  C12Q2521/101 ,  C12Q2521/543 ,  C12Q2535/122 ,  C12Q2563/113 ,  C12Q2563/116 ,  C12Q2563/157 ,  C12Q2565/607

    Abstract: Real time electronic sequencing methods, devices, and systems are described. Arrays of nanoscale electronic elements comprising capacitive devices with one or two electrodes, or arrays of nanoFET devices are used to provide sequence information about a template nucleic acid in a polymerase-template complex bound proximate to the nanoscale electronic elements or to the substrate proximate to the nanoscale electronic element. A sequencing reaction mixture comprising nucleotide analogs having impedance labels, capacitive labels, or conductivity labels is introduced to the array of nanoscale electronic elements comprising capacitive devices or nanoFETs under conditions of polymerase mediated nucleic acid synthesis. The time sequence of incorporation of nucleotide analogs is determined by identifying the types of labels of the nucleotide analogs that are incorporated into the growing strand using measured impedance, conductivity, or capacitance.

    PROTECTED FLUORESCENT REAGENT COMPOUNDS
    6.
    发明公开
    PROTECTED FLUORESCENT REAGENT COMPOUNDS 审中-公开
    GESCHÜTZTEFLUORESZIERENDE REAGENZVERBINDUNGEN

    公开(公告)号：EP3030683A4

    公开(公告)日：2017-05-03

    申请号：EP14834734

    申请日：2014-08-05

    Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

    Inventor： SEBO LUBOMIR ,  HANES JEREMIAH ,  SHEN GENE ,  BROGLEY LOUIS ,  YUE STEPHEN ,  ZHENG FRANK ,  LAPIN YURI ,  LYLE JOHN ,  OSUNA HONEY ,  FEDOROV ANDREI

    IPC: C12Q1/68 ,  C07F9/6558 ,  C07F9/6561 ,  C07H19/10 ,  G01N21/64 ,  G01N23/223

    CPC classification number: C07H19/10 ,  C07F9/65586 ,  C07F9/65616

    Abstract: Protected fluorescent reagent compounds and their methods of synthesis are provided. The compounds are useful in various fluorescence-based analytical methods, including the analysis of highly multiplexed optical reactions in large numbers at high densities, such as single molecule real time nucleic acid sequencing reactions. The compounds contain fluorescent dye elements, that allow the compounds to be detected with high sensitivity at desirable wavelengths, binding elements, that allow the compounds to be recognized specifically by target biomolecules, and protective shield elements, that decrease undesirable contacts between the fluorescent dye elements and the bound target biomolecules and that therefore decrease photodamage of the bound target biomolecules by the fluorescent dye elements.

    Abstract translation: 提供受保护的荧光试剂化合物及其合成方法。 这些化合物可用于各种基于荧光的分析方法，包括高密度分析大量高度多重光学反应，如单分子实时核酸测序反应。 这些化合物含有荧光染料元素，可以在理想的波长下以高灵敏度检测化合物，允许化合物特异性地被目标生物分子识别的结合元件和保护性屏蔽元​​件，其减少荧光染料元素之间的不希望的接触 和结合的目标生物分子，并因此减少了荧光染料元件对结合的目标生物分子的光损伤。

    BIS-BIOTINYLATION TAGS
    7.
    发明公开
    BIS-BIOTINYLATION TAGS 审中-公开
    BIS-BIOTINYLIERUNGSETIKETTEN

    公开(公告)号：EP3008094A4

    公开(公告)日：2017-04-12

    申请号：EP14810199

    申请日：2014-06-12

    Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

    Inventor： MILLER ERIK ,  KAMTEKAR SATWIK ,  BJORNSON KEITH ,  HANES JEREMIAH

    IPC: C07K19/00 ,  G01N33/68

    CPC classification number: G01N33/58 ,  C07K14/36

    Abstract: Multi-biotinylated reactants are provided which can be used in divalent complexes for various applications such as colocalization, labeling, immobilization, and purification. Methods for constructing, purifying, and using the bis-biotinylated reactants are also provided. In certain embodiments, two bis-biotinylated reactants are bound to a single streptavidin tetramer to provide a complex having a 1:1 stoichiometry with respect to the bis-biotinylated reactants.

    Abstract translation: 提供多生物素化反应物，其可以用于二价复合物用于各种应用，例如共定位，标记，固定和纯化。 还提供了构建，纯化和使用双生物素化反应物的方法。 在某些实施方案中，两种双生物素化的反应物与单个链霉抗生物素蛋白四聚体结合以提供相对于双生物素化反应物具有1：1化学计量的复合物。

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