公开(公告)号：WO1994006932A1

公开(公告)日：1994-03-31

申请号：PCT/US1993008612

申请日：1993-09-10

Applicant: PACKARD INSTRUMENT COMPANY, INC.

Inventor： PACKARD INSTRUMENT COMPANY, INC. ,  ROELANT, Chris

IPC: C12Q01/06

CPC classification number: G01N33/5014 ,  C12Q1/06 ,  Y10S435/968 ,  Y10S435/975 ,  Y10S436/904 ,  Y10S436/905

Abstract: The present invention provides a process of quantifying the number of viable cells in an aqueous suspension of cells using an energy-emitting non-hazardous probe and a probe-trigger. The process provides quantification data in short periods of time without the use of hazardous materials. A process of the present invention can also be used to quantify negatively charged particle number, assay for cytotoxicity, assay for cell proliferation and assay for cell differentiation. Still further, the present invention provides an assay kit for quantification of cells or negatively charged particles.

Abstract translation: 本发明提供了使用能量发射非危险探针和探针触发剂来定量细胞水性悬浮液中活细胞数目的方法。 该过程在短时间内提供量化数据，而不使用危险物质。 本发明的方法还可以用于定量带负电的粒子数，测定细胞毒性，细胞增殖测定和细胞分化测定。 此外，本发明提供了用于定量细胞或带负电粒子的测定试剂盒。

公开(公告)号：EP0660876A1

公开(公告)日：1995-07-05

申请号：EP93921526.0

申请日：1993-09-10

Applicant: Packard Instrument Company, Inc.

Inventor： ROELANT, Chris

IPC: C12Q1 ,  G01N33

CPC classification number: G01N33/5014 ,  C12Q1/06 ,  Y10S435/968 ,  Y10S435/975 ,  Y10S436/904 ,  Y10S436/905

Abstract: The present invention provides a process of quantifying the number of viable cells in an aqueous suspension of cells using an energy-emitting non-hazardous probe and a probe-trigger. The process provides quantification data in short periods of time without the use of hazardous materials. A process of the present invention can also be used to quantify negatively charged particle number, assay for cytotoxicity, assay for cell proliferation and assay for cell differentiation. Still further, the present invention provides an assay kit for quantification of cells or negatively charged particles.