公开(公告)号：CA2201428A1

公开(公告)日：1996-04-11

申请号：CA2201428

申请日：1995-10-02

Applicant: PATHOGENESIS CORP

Inventor： STOVER CHARLES KENDALL ,  HICKEY MARK JEFFREY

IPC: C12N9/02 ,  C12N15/74 ,  C12Q1/02 ,  C12Q1/68 ,  C12Q1/6897 ,  C12N1/21 ,  C12Q1/66

Abstract: This invention relates to a method of quantifying bacteria in vivo or in vitro using bacterial reporter strains. In particular this invention provides a method utilizing mycobacterial reporter strains that permits rapid screening for in vivo antimycobacterial activity of various compositions. In addition this invention provides for particular mycobacterial reporter strains expressing the FFlux gene at levels sufficiently high to allow detection in tissue homogenates without lysis or concentration of the bacteria.

公开(公告)号：AU4979696A

公开(公告)日：1996-09-04

申请号：AU4979696

申请日：1996-02-15

Applicant: PATHOGENESIS CORP

Inventor： STOVER CHARLES KENDALL ,  MAHAIRAS GREGORY G

IPC: A61K38/00 ,  C07K14/35 ,  C12N1/21 ,  C12N15/74 ,  C12Q1/68 ,  G01N33/569 ,  G01N33/53 ,  C12P19/34 ,  C12N5/10 ,  C07K5/00 ,  C07K14/00 ,  C07K16/00 ,  C07H21/02 ,  C07H21/04

Abstract: The present invention provides specific genetic deletions that result in an avirulent phenotype of a mycobacterium. These deletions may be used as phenotypic markers of providing a means for distinguishing between disease-producing and non-disease producing mycobacteria.