公开(公告)号：US20240377404A1

公开(公告)日：2024-11-14

申请号：US18613432

申请日：2024-03-22

Applicant: Promega Corporation

Inventor： Rachel Friedman OHANA ,  Thomas KIRKLAND ,  Robin HURST ,  Mark KLEIN ,  Matt LARSEN ,  Hui WANG ,  Wenhui ZHOU ,  Thomas MACHLEIDT

IPC: G01N33/58 ,  B01J23/46 ,  B01J27/26 ,  B01J31/02 ,  C09K11/06 ,  C12N9/02 ,  G01N21/76

Abstract: Provided herein are systems, methods, and compositions for bioluminescence-triggered photocatalytic activation of molecular entities in a proximity-dependent manner, which can be actuated within biological systems. In particular, provided herein are bioluminescent proteins or complexes, luminophore substrates thereof, photocatalysts, and activatable molecular entities incorporating light-responsive moieties that restrict their activity; systems thereof; and methods for catalytically activating the activatable molecular entities via bioluminescence-triggered catalysis.

公开(公告)号：US20240302339A1

公开(公告)日：2024-09-12

申请号：US18600390

申请日：2024-03-08

Applicant: PROMEGA CORPORATION

Inventor： Ian MAROZAS ,  Zhiyang ZENG ,  Wenhui ZHOU ,  Mike VALLEY ,  Jolanta VIDUGIRIENE ,  James CALI ,  Tony LAUER

IPC: G01N31/22 ,  C12M1/34 ,  G01N21/64

CPC classification number: G01N31/225 ,  C12M41/46 ,  G01N21/6408 ,  G01N2201/06193

Abstract: Provided herein are assays utilizing oxygen-sensitive fluorescent materials for the detection of oxygen. In particular, oxygen-sensitive fluorescent particles are provided for monitoring the oxygen consumption rate and metabolic fitness of living cells.

公开(公告)号：US20140093894A1

公开(公告)日：2014-04-03

申请号：US14032420

申请日：2013-09-20

Applicant: PROMEGA CORPORATION

Inventor： Helene A. BENINK ,  James J. CALI ,  Sarah DUELLMAN ,  Dieter KLAUBERT ,  Donna LEIPPE ,  Martha O'BRIEN ,  John SHULTZ ,  Jolanta VIDUGIRIENE ,  Wenhui ZHOU ,  Mary SOBOL

IPC: C12Q1/66 ,  C12Q1/32

CPC classification number: C12Q1/32 ,  C07D277/68 ,  C07D417/04 ,  C07D487/04 ,  C07D493/10 ,  C07D497/10 ,  C07F9/65583 ,  C07F9/6561 ,  C12Q1/66 ,  G01N21/763 ,  G01N33/5038 ,  G01N33/5735 ,  G01N33/582

Abstract: The present invention provides compounds and methods for assaying redox state of metabolically active cells and methods for assaying enzyme activity and/or metabolite level by coupling to redox defining co-factor NAD(P)/NAD(P)H measurement.

Abstract translation: 本发明提供了用于测定代谢活性细胞的氧化还原状态的化合物和方法，以及通过与氧化还原定义的辅因子NAD（P）/ NAD（P）H测量偶联来测定酶活性和/或代谢物水平的方法。