公开(公告)号：US20220064710A1

公开(公告)日：2022-03-03

申请号：US17464476

申请日：2021-09-01

Applicant: Promega Corporation

Inventor： Ce SHI ,  Nathan FEIRER ,  Michael SCURRIA ,  Subhanjan MONDAL ,  Said GOUELI ,  Thomas KIRKLAND ,  James J. CALI ,  Poncho MEISENHEIMER

IPC: C12Q1/686 ,  C07D221/12 ,  C07F15/00 ,  C07D403/04 ,  C07D513/06 ,  C12Q1/70 ,  C12Q1/689

Abstract: The present disclosure includes compounds, compositions, and methods for nucleic acid amplification reactions. In particular, the present disclosure provides compounds, compositions, and methods for viability PCR (vPCR) applications, in which the compounds that selectively bind to nucleic acids from non-viable cells.

公开(公告)号：US20140093894A1

公开(公告)日：2014-04-03

申请号：US14032420

申请日：2013-09-20

Applicant: PROMEGA CORPORATION

Inventor： Helene A. BENINK ,  James J. CALI ,  Sarah DUELLMAN ,  Dieter KLAUBERT ,  Donna LEIPPE ,  Martha O'BRIEN ,  John SHULTZ ,  Jolanta VIDUGIRIENE ,  Wenhui ZHOU ,  Mary SOBOL

IPC: C12Q1/66 ,  C12Q1/32

CPC classification number: C12Q1/32 ,  C07D277/68 ,  C07D417/04 ,  C07D487/04 ,  C07D493/10 ,  C07D497/10 ,  C07F9/65583 ,  C07F9/6561 ,  C12Q1/66 ,  G01N21/763 ,  G01N33/5038 ,  G01N33/5735 ,  G01N33/582

Abstract: The present invention provides compounds and methods for assaying redox state of metabolically active cells and methods for assaying enzyme activity and/or metabolite level by coupling to redox defining co-factor NAD(P)/NAD(P)H measurement.

Abstract translation: 本发明提供了用于测定代谢活性细胞的氧化还原状态的化合物和方法，以及通过与氧化还原定义的辅因子NAD（P）/ NAD（P）H测量偶联来测定酶活性和/或代谢物水平的方法。