公开(公告)号：WO1996014423A1

公开(公告)日：1996-05-17

申请号：PCT/US1995014170

申请日：1995-11-01

Applicant: RESEARCH CORPORATION TECHNOLOGIES, INC.

Inventor： RESEARCH CORPORATION TECHNOLOGIES, INC. ,  AFANASIEV, Boris, N. ,  CARLSON, Jonathan, O. ,  BEATY, Barry ,  HIGGINS, David, R. ,  THIBAULT, Kelly, J.

IPC: C12N15/86

CPC classification number: C12N15/86 ,  C12N2750/14043

Abstract: The present invention is directed to use of Aedes aegypti densonucleosis virus (Aedes DNV or AeDNV) as a heterologous gene expression system. The invention described herein provides promoters from the Aedes aegypti genome such as the p.05, p7 and p61 promoters to generate expression vectors with cloning sites for insertion of heterologous genes. The invention further provides expression vectors comprising an AeDNV promoter operably linked to a heterologous gene and insect host cells comprising the vectors of the invention. Methods of producing recombinant proteins utilizing the vectors of the invention are also provided. The present invention further provides a kit containing the vectors of the invention for production of a recombinant protein.

Abstract translation: 本发明涉及埃及伊蚊密集型核酸病毒（Aedes DNV或AeDNV）作为异源基因表达系统的用途。 本文描述的本发明提供了来自埃及伊蚊的基因组的启动子，例如p.05，p7和p61启动子，以产生具有用于插入异源基因的克隆位点的表达载体。 本发明还提供了包含可操作地连接到异源基因的AeDNV启动子和包含本发明载体的昆虫宿主细胞的表达载体。 还提供了利用本发明的载体生产重组蛋白的方法。 本发明还提供了含有用于产生重组蛋白的本发明载体的试剂盒。

公开(公告)号：EP0789775A1

公开(公告)日：1997-08-20

申请号：EP95939696.0

申请日：1995-11-01

Applicant: Research Corporation Technologies, Inc

Inventor： AFANASIEV, Boris, N. ,  CARLSON, Jonathan, O. ,  BEATY, Barry ,  HIGGINS, David, R. ,  THIBAULT, Kelly, J.

IPC: C12N15

CPC classification number: C12N15/86 ,  C12N2750/14043

Abstract: The present invention is directed to use of Aedes aegypti densonucleosis virus (Aedes DNV or AeDNV) as a heterologous gene expression system. The invention described herein provides promoters from the Aedes aegypti genome such as the p.05, p7 and p61 promoters to generate expression vectors with cloning sites for insertion of heterologous genes. The invention further provides expression vectors comprising an AeDNV promoter operably linked to a heterologous gene and insect host cells comprising the vectors of the invention. Methods of producing recombinant proteins utilizing the vectors of the invention are also provided. The present invention further provides a kit containing the vectors of the invention for production of a recombinant protein.