公开(公告)号：WO1994000473A2

公开(公告)日：1994-01-06

申请号：PCT/US1993006277

申请日：1993-06-30

Applicant: RESEARCH CORPORATION TECHNOLOGIES, INC.

Inventor： RESEARCH CORPORATION TECHNOLOGIES, INC. ,  WICKSTROM, Eric ,  LEBEDEV, Alexander V.

IPC: C07H21/00

CPC classification number: C12N15/113 ,  C07H21/00 ,  C12N2310/321 ,  C12Q1/6813 ,  C12Q1/6886

Abstract: The present invention provides a method for making R stereospecific alkyl- and aryl-phosphonate linkages between nucleotides. These methods can be used for automated synthesis of oligonucleotides having sequential R stereospecific alkyl- and aryl-phosphonate linkages. The present invention is also directed to the oligonucleotides having several sequential R phosphonate linkages which were produced by the subject methods. Moreover, the present invention provides methods for using the subject oligonucleotides, including methods for regulating the biosynthesis of a DNA, an RNA or a protein and methods for detecting and isolating complementary nucleic acid targets.

Abstract translation: 本发明提供了在核苷酸之间制备R立体特异性烷基 - 和芳基 - 膦酸酯键的方法。 这些方法可用于自动合成具有顺序R立体特异性烷基 - 和芳基 - 膦酸酯键的寡核苷酸。 本发明还涉及通过本发明方法制备的具有多个连续的R膦酸酯键的寡核苷酸。 此外，本发明提供了使用本发明的寡核苷酸的方法，包括调节DNA，RNA或蛋白质的生物合成的方法以及用于检测和分离互补核酸靶的方法。