公开(公告)号：JPH04232463A

公开(公告)日：1992-08-20

申请号：JP16620191

申请日：1991-06-12

Applicant: SCLAVO SPA

Inventor： PAORO FUABURITSUTSUI ,  FURANCHIESUKO DOTSUNIINI

IPC: C12Q1/37 ,  C12Q1/42 ,  G01N33/53 ,  G01N33/531 ,  G01N33/564 ,  G01N33/569 ,  G01N33/573

Abstract: PURPOSE: To make it possible to bond IgM and the like by the operation of collagenase contained by using buffer solution having specific pH and containing collagenase existing in specific concentration with TV type collagnase to dilute serum. CONSTITUTION: Buffer liquid contains collagenase, has a pH of 7.0 to 7.4 so that the collagenase is IV type collagenase, which is existed in concentration of 1000 to 3000 U/l. The liquid contains, for example, 1.09g/1 of sodium biphosphate anhydride, 0.37g/l of sodium phosphate hydride, 8.5g/l of sodium chloride, 10g/l of borine serum albumin, 0.5g/l of Brij 35, 0.5/l of aggregate human IgG, 1500U/l of collagenase IV, and 1.00g/l of sodium azide. When the liquid is used to dilute serum, the collagnase enzyme contained therein is operated at the amino acid sequence of Clq capable of bonding IgM and the like.