公开(公告)号：US09990464B1

公开(公告)日：2018-06-05

申请号：US14049863

申请日：2013-10-09

Applicant: SENSIQ TECHNOLOGIES, INC.

Inventor： John Gerard Quinn

IPC: C12M1/34 ,  G06F19/12 ,  G01N33/557 ,  G01N35/08 ,  G01N33/53 ,  G01N21/55 ,  G01N21/552 ,  G01N35/10 ,  G01N21/05

CPC classification number: G06F19/12 ,  B01L2300/0636 ,  G01N21/272 ,  G01N21/55 ,  G01N21/553 ,  G01N33/53 ,  G01N35/08 ,  G01N35/085 ,  G01N35/1097 ,  G01N2021/058 ,  G01N2035/00702

Abstract: Dispersion injection methods for determining biomolecular interaction parameters in label-free biosensing systems are provided. The methods generally relate to the use of a single analyte injection that generates a smoothly-varying concentration gradient via dispersion en route to a sensing region possessing an immobilized binding partner. The present method incorporates the use of an internal standard which provides a reference as to the dispersion conditions present which can then be used to calculate an effective diffusion coefficient for the analyte of interest based on a universal calibration function. The effective diffusion coefficient can then be incorporated into the appropriate dispersion model to provide a calibrated dispersion model. The calibrated dispersion model can then be incorporated into the desired interaction model to provide a reliable representation of the analyte concentration at the sensing region at any time during the injection. The use of the internal standard and universal calibration function permit use of a wide range of injection conditions which may not otherwise be consistent with a particular dispersion model. Thus, the present methods allow for higher flow rates and lower sample volumes thereby increasing assay speed and decreasing sample consumption.