Abstract:
Disclosed herein are methods and compositions for detecting amplicon generated using loop-mediated amplification (LAMP). In particular, the invention relates to compositions comprising molecular beacons and/or LAMP primers and methods for generating and detecting LAMP amplicons. In particular, molecular beacons that bind to novel regions of a LAMP amplicon, and methods of using these probes, are provided.
Abstract:
The invention provides methods and compositions for the detection of Chlamydia trachomatis in a test sample. Its presence or absence in the sample is determined by nucleic acid based testing methods using primers and/or probes and or molecular beacons that bind to the 16S or 23S ribosomal genes or gene transcripts.