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1.发明公开PREPARATION METHOD AND PREPARATION DEVICE FOR SAMPLE FOR NUCLEIC ACID AMPLIFICATION REACTION 有权METHOD AND APPARATUS FOR制备样品用于核酸扩增
公开(公告)号：EP2878673A4
公开(公告)日：2016-04-06
申请号：EP13823547
申请日：2013-06-10
Applicant: SONY CORP
Inventor： YOTORIYAMA TASUKU , ITO KAZUMINE , SAKAMOTO NAOHISA , NAKAMURA TOMOHIKO
CPC classification number: C12Q1/6806 , G01N27/447 , G01N2001/4038
Abstract: There is provided a method for preparing a sample for a nucleic acid amplification reaction, the method including a heating step of applying heat to a nucleic acid-containing sample, and an electrodialysis step of bringing an electrical conductivity of the sample to 2,000 µS/cm or less.
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公开(公告)号：EP2423690A4
公开(公告)日：2016-06-29
申请号：EP10767181
申请日：2010-04-19
Applicant: SONY CORP
Inventor： YOTORIYAMA TASUKU , SAKAMOTO NAOHISA , SEGAWA YUJI , TANAKA SATOMI
CPC classification number: B01L3/502715 , B01L3/502723 , B01L2200/027 , B01L2200/0605 , B01L2200/0673 , B01L2200/142 , B01L2300/0816 , B01L2300/0864 , B01L2400/0487 , B01L2400/049
Abstract: A sample solution introduction kit and a sample solution injector capable of introducing sample solution while reducing the rate of occurrence of an air bubble and having a simplified structure are proposed. A sample solution introduction kit includes a plate-like member and a sample solution injector. The plate-like member has a plurality of spaces formed therein and serving as reaction field and a communication space that communicates with the plurality of spaces therein and that has a portion defining an opening formed in a surface of the plate-like member, and the sample solution injector includes a container containing the sample solution, a tube that communicates with the bottom of the container and that is insertable into the opening, a stopper removably fitted into an opening formed at a top end of the tube, and liquid held in the container, and the liquid is insoluble in the sample solution and is lighter than the sample solution.
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3.发明公开NUCLEIC ACID PURIFICATION METHOD, NUCLEIC ACID EXTRACTION METHOD, AND NUCLEIC ACID PURIFICATION KIT 有权PROCEDURE核酸纯化，核酸提取方法和试剂盒用于纯化核酸
公开(公告)号：EP2757156A4
公开(公告)日：2015-08-26
申请号：EP12832581
申请日：2012-08-28
Applicant: SONY CORP
Inventor： NAKAMURA TOMOHIKO , SAKAMOTO NAOHISA , YOTORIYAMA TASUKU , ITO KAZUMINE
CPC classification number: C12N15/101 , B01D15/34 , B01D15/362 , B01D15/363 , B01J39/04 , B01J41/04 , C12N15/09 , C12Q1/68 , C12Q1/6806 , G01N30/88 , G01N30/96 , G01N2030/8827 , C12Q2523/308 , B01J39/043 , B01J41/043 , B01J39/05 , B01J41/05
Abstract: [Object] To provide a method of purifying nucleic acids where the operation is simple and the nucleic acids can be extracted in a short time with high efficiency. [Solving Means] A method of purifying nucleic acids including the step of adsorbing substances in a sample containing nucleic acids with an ion exchange resin 10 including a positive ion exchange resin and a negative ion exchange resin. As the positive ion exchange resin, a first positive ion exchange resin and a second positive ion exchange resin having an exclusion limit molecular weight lower than that of the first positive ion exchange resin may be used.
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公开(公告)号：JP2011085578A
公开(公告)日：2011-04-28
申请号：JP2010148819
申请日：2010-06-30
Inventor： MACHIDA KENZO , KISHII NORIYUKI , SAKAMOTO NAOHISA , ICHIMURA MARI , ITO KAZUTAKA , KISHIMOTO TAKUYA
IPC: G01N21/64 , G01N21/78 , G01N33/543
CPC classification number: G01N33/54366 , G01N21/6452
Abstract: PROBLEM TO BE SOLVED: To provide a microbead analysis method capable of highly accurately detecting fluorescence from a fluorescent material or the like used for labeling a target substance without requiring complicated preparation processes by excluding the effects of noise fluorescence caused by an identification pattern. SOLUTION: A microbead 1 is formed in a columnar shape comprising an upper surface 11 and a lower surface 12 opposed to each other approximately in parallel with each other and sides 13 continuous to these surfaces. The identification pattern is formed at least in either the upper surface 11 or the lower surface 12, and a solid phase of a substance having affinity to a substance to be detected is formed in the surface of the microbead 1. Fluorescence to be detected in the surface of the microbead 1 on the basis of the interaction between the substance to be detected and the substance having affinity to the substance to be detected is detected from a region including both of a region 112 in which the identification pattern is not formed among the upper surface 11 and the lower surface 12 and the sides 13 in the microbead analysis method. COPYRIGHT: (C)2011,JPO&INPIT
Abstract translation: 解决方案：提供一种能够高效准确地检测用于标记目标物质的荧光材料等的荧光的微珠分析方法，而不需要复杂的制备方法，通过排除由识别图案引起的噪声荧光的影响 。 解决方案：微珠1形成为包括彼此大致平行地彼此相对的上表面11和下表面12以及与这些表面连续的侧面13的柱状。 识别图案至少形成在上表面11或下表面12中，并且在微珠1的表面中形成对被检测物质具有亲和性的物质的固相。荧光在 基于待检测物质与对待检测物质具有亲和性的物质之间的相互作用的微珠1的表面，从包含未形成识别图案的区域112的区域中检测出来 表面11和下表面12以及侧面13的微珠分析方法。 版权所有（C）2011，JPO＆INPIT
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5.发明专利Nucleic acid purification method, nucleic acid extraction method, and nucleic acid purification kit 有权核酸纯化方法，核酸提取方法和核酸纯化试剂盒
公开(公告)号：JP2013059275A
公开(公告)日：2013-04-04
申请号：JP2011198949
申请日：2011-09-13
Inventor： NAKAMURA TOMOHIKO , SAKAMOTO NAOHISA , SETORIYAMA TASUKU , ITO KAZUTAKA
CPC classification number: C12N15/101 , B01D15/34 , B01D15/362 , B01D15/363 , B01J39/04 , B01J41/04 , C12N15/09 , C12Q1/68 , C12Q1/6806 , G01N30/88 , G01N30/96 , G01N2030/8827 , C12Q2523/308 , B01J39/043 , B01J41/043 , B01J39/05 , B01J41/05
Abstract: PROBLEM TO BE SOLVED: To provide a nucleic acid purification method which involves simple operations and can purify the nucleic acid at high efficiency in a short time.SOLUTION: The nucleic acid purification method includes a procedure of adsorbing a substance, included in a sample containing nucleic acid, by an ion exchange resin 10, wherein a cation exchange resin and an anion exchange resin are used for the ion exchange resin 10. A first cation exchange resin and a second cation exchange resin having a lesser exclusion limit molecular weight than that of the first cation exchange resin may be used for the cation exchange resin.
Abstract translation: 待解决的问题：提供涉及简单操作并且可以在短时间内以高效率纯化核酸的核酸纯化方法。 解决方案：核酸纯化方法包括通过离子交换树脂10吸附包含在含有核酸的样品中的物质的方法，其中阳离子交换树脂和阴离子交换树脂用于离子交换树脂 阳离子交换树脂可以使用第一阳离子交换树脂和具有比第一阳离子交换树脂更低排斥极限分子量的第二阳离子交换树脂。 版权所有（C）2013，JPO＆INPIT
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Patent Agency Ranking6.发明专利Carrier for separating nucleic acid, micro flow channel system, nucleic acid-separating apparatus, method for separating nucleic acid and method for making capturing strand sold-phased 审中-公开用于分离核酸的载体，微流通道系统，核酸分离装置，分离核酸的方法和制备捕获条的方法
公开(公告)号：JP2012024017A
公开(公告)日：2012-02-09
申请号：JP2010165507
申请日：2010-07-23
Inventor： SAKAMOTO NAOHISA , ICHIMURA MARI , ONISHI MICHIHIRO , KISHII NORIYUKI
Abstract: PROBLEM TO BE SOLVED: To provide a technique for efficiently separating a target nucleic acid strand in a micro flow channel packed with a carrier for separating the nucleic acid.SOLUTION: The carrier for separating the nucleic acid is sold-phased by sulfonamide bond of a sulfone group introduced to the surface of a porous carrier and an amino group of the captured strand having a base sequence complementary to the target nucleic acid strand.
Abstract translation: 要解决的问题：提供一种用于有效分离装有用于分离核酸的载体的微流通道中的靶核酸链的技术。 解决方案：用于分离核酸的载体通过引入到多孔载体的表面的砜基的磺酰胺键进行销售，并且捕获的链的氨基具有与靶核酸链互补的碱基序列 。 版权所有（C）2012，JPO＆INPIT
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公开(公告)号：JP2014199206A
公开(公告)日：2014-10-23
申请号：JP2013074628
申请日：2013-03-29
Inventor： SAKAMOTO NAOHISA , MATSUMOTO MASAHIRO , WATANABE HIDETOSHI , ONISHI MICHIHIRO , KATO YOSHIAKI , WATANABE TOSHIO
CPC classification number: G01N27/447 , G01N30/6095 , G01N2030/8827
Abstract: 【課題】液体の充填が完了する時間のばらつきを抑制可能なマイクロチップの提供。【解決手段】液体が注入される導入部と、前記液体が供給される複数の分析領域と、前記液体が前記導入部から前記複数の分析領域のそれぞれに同じタイミングで供給されるように形成された流路と、を備えるマイクロチップを提供する。このマイクロチップでは、導入部に液体が注入されると、その液体が流路を通流し、複数の分析領域のそれぞれに同じタイミングで到達する。【選択図】図1
Abstract translation: 要解决的问题：提供一种能够抑制液体填充完成时间的波动的微芯片。解决方案：提供一种微芯片，包括：注入液体的入口部分; 供给液体的多个分析区域; 以及流路，其形成为在与所述入口部相同的时刻将液体供给到所述多个分析区域中的每一个。 在微芯片中，当向入口部注入液体时，液体流过流路并以相同的时刻到达各个分析区域。
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公开(公告)号：JP2014171432A
公开(公告)日：2014-09-22
申请号：JP2013047143
申请日：2013-03-08
Inventor： SAKAMOTO NAOHISA , NAKAMURA TOMOHIKO , ITO KAZUTAKA , SETORIYAMA TASUKU
CPC classification number: G01N27/44756 , C12N15/10 , C12Q1/6806
Abstract: PROBLEM TO BE SOLVED: To provide a method for preparing a specimen that contains nucleic acids so as to be in a better state for analyzing the nucleic acids, easily without going through troublesome steps.SOLUTION: An apparatus for preparing nucleic acids is provided which includes an anode, a cathode, and a space formed between the anode and the cathode, in which the space includes a first porous film provided at an anode side, a second porous film provided at a cathode side, an inlet port which is proved in a region sandwiched between the first porous film and the second porous film and which introduces a specimen containing proteins and nucleic acids into the region, and a gel filter provided between the inlet port and the first porous film.
Abstract translation: 要解决的问题：提供一种制备含有核酸以便分析核酸的更好状态的样品的方法，容易地不经过麻烦的步骤。提供了一种制备核酸的装置，其包括 阳极，阴极和形成在阳极和阴极之间的空间，其中空间包括设置在阳极侧的第一多孔膜，设置在阴极侧的第二多孔膜，在 区域夹在第一多孔膜和第二多孔膜之间，并将含有蛋白质和核酸的样品引入该区域，以及设置在入口和第一多孔膜之间的凝胶过滤器。
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9.发明专利
公开(公告)号：JP2011145276A
公开(公告)日：2011-07-28
申请号：JP2010154877
申请日：2010-07-07
Inventor： SAKAMOTO NAOHISA , ITO KAZUTAKA , KISHII NORIYUKI
CPC classification number: B01L3/502761 , B01L3/502753 , B01L2200/0668 , B01L2300/0681 , B01L2300/0822 , B01L2300/0877 , B01L2400/0487 , G01N15/1463 , G01N33/582
Abstract: PROBLEM TO BE SOLVED: To provide a cell for testing, capable of preventing microbeads from overlapping. SOLUTION: A cell 1 for testing microbeads is arranged in a housing space 39 so that the microbeads 7 are not overlapped each other because the distance between a support substrate 11 and a cover 21 is greater than the thickness D of the microbeads 7 and smaller than twice the thickness D of the microbeads 7. Thus, the cell 1 for testing the microbeads normally picks up respective images of the microbeads 7 and keeps a high analysis accuracy. COPYRIGHT: (C)2011,JPO&INPIT
Abstract translation: 要解决的问题：提供能够防止微珠重叠的用于测试的细胞。 解决方案：用于测试微珠的电池1布置在容纳空间39中，使得微珠7彼此不重叠，因为支撑基板11和盖21之间的距离大于微珠7的厚度D 并且小于微珠7的厚度D的两倍。因此，用于测试微珠的电池1通常拾取微珠7的各自的图像并保持高分析精度。 版权所有（C）2011，JPO＆INPIT
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10.发明专利
公开(公告)号：JP2014098595A
公开(公告)日：2014-05-29
申请号：JP2012249726
申请日：2012-11-13
Inventor： SETORIYAMA TASUKU , SEGAWA YUJI , ONISHI MICHIHIRO , KATO YOSHIAKI , ABE TOMOTERU , MACHIDA KENZO , MATSUMOTO MASAHIRO , NAKAMURA TOMOHIKO , SAKAMOTO NAOHISA , WATANABE HIDETOSHI , ANAGUCHI TAKAKI , AKITA MASAYOSHI , MIYAJI MASAHIRO
CPC classification number: B01L3/502715 , B01L3/502753 , B01L7/00 , B01L2200/027 , B01L2300/044 , B01L2300/0672 , B01L2300/0681 , B01L2300/0816 , B01L2300/0832 , B01L2300/0835 , B01L2300/1822 , B01L2400/0478 , B01L2400/0481 , B01L2400/049
Abstract: PROBLEM TO BE SOLVED: To provide a sample liquid injection tool for simply performing pretreatment of a sample liquid.SOLUTION: There is provided a sample liquid injection tool including a reservoir section configured to store a sample liquid, a channel having one end protruding from an outer surface and configured to discharge the sample liquid therein from a protrusion end to an outside, and a heating unit and a filter installed between the reservoir section and the channel to enable passage of the liquid. The sample liquid injection tool enables simple heat treatment and filtration of a sample liquid.
Abstract translation: 要解决的问题：提供一种用于简单地执行样品液体的预处理的样品液体注入工具。解决方案：提供了一种样品液体注入工具，其包括构造成存储样品液体的储存部分，具有从一个 外表面并且被构造成将其中的样品液体从突出端排出到外部;以及加热单元和过滤器，其安装在储存器部分和通道之间以使液体能够通过。 样品液体注入工具能够对样品液进行简单的热处理和过滤。
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