Abstract:
MICROCHIP FOR NUCLEIC ACID AMPLIFICATION REACTION AND A METHOD OF MANUFACTURING THE SAMEAbstract [Abstract][Object] To provide a microchip for a nucleic acid amplification reaction which allows high-precision analysis by a simple method.[Solving Means] There is provided a microchip A for a nucleic acid amplification reaction including anentrance 1 through which a liquid enters from the outside, a plurality of wells 41, 42 and 43 configured to function as reaction sites of nucleic acidamplification reaction, and flow channels 2 and 3 through which the liquid entered from the entrance 1 is fed into each of the wells 41, 42 and 43, in which aplurality of reagents needed for the reaction are laminated and anchored in a prescribed order in each well 41, 42 and 43.FIG. 1
Abstract:
There is provided a method for preparing a sample for a nucleic acid amplification reaction, the method including a heating step of applying heat to a nucleic acid-containing sample, and an electrodialysis step of bringing an electrical conductivity of the sample to 2,000 µS/cm or less.
Abstract:
A sample solution introduction kit and a sample solution injector capable of introducing sample solution while reducing the rate of occurrence of an air bubble and having a simplified structure are proposed. A sample solution introduction kit includes a plate-like member and a sample solution injector. The plate-like member has a plurality of spaces formed therein and serving as reaction field and a communication space that communicates with the plurality of spaces therein and that has a portion defining an opening formed in a surface of the plate-like member, and the sample solution injector includes a container containing the sample solution, a tube that communicates with the bottom of the container and that is insertable into the opening, a stopper removably fitted into an opening formed at a top end of the tube, and liquid held in the container, and the liquid is insoluble in the sample solution and is lighter than the sample solution.
Abstract:
[Object] To provide a method of purifying nucleic acids where the operation is simple and the nucleic acids can be extracted in a short time with high efficiency. [Solving Means] A method of purifying nucleic acids including the step of adsorbing substances in a sample containing nucleic acids with an ion exchange resin 10 including a positive ion exchange resin and a negative ion exchange resin. As the positive ion exchange resin, a first positive ion exchange resin and a second positive ion exchange resin having an exclusion limit molecular weight lower than that of the first positive ion exchange resin may be used.
Abstract:
[Object] To provide a microchip for a nucleic acid amplification reaction which allows high-precision analysis by a simple method. [Solving Means] There is provided a microchip A for a nucleic acid amplification reaction including an entrance 1 through which a liquid enters from the outside, a plurality of wells 41, 42 and 43 configured to function as reaction sites of nucleic acid amplification reaction, and flow channels 2 and 3 through which the liquid entered from the entrance 1 is fed into each of the wells 41, 42 and 43, in which a plurality of reagents needed for the reaction are laminated and anchored in a prescribed order in each well 41, 42 and 43.