公开(公告)号：EP0733706A2

公开(公告)日：1996-09-25

申请号：EP96104542.4

申请日：1996-03-21

Applicant: ST. LOUIS UNIVERSITY

Inventor： Chinnadurai, Govindaswamy

IPC: C12N15/12 ,  C07K14/47 ,  C07K14/075 ,  C12N15/33 ,  C12N15/62 ,  A61K38/16 ,  G01N33/68 ,  C12N1/21 ,  C12N5/10

CPC classification number: C07K14/005 ,  A61K38/00 ,  C07K14/4747 ,  C07K2319/00 ,  C12N2710/10322

Abstract: Adenovirus E1B 19 kD protein protects against cell death induced by viral infection and certain external physical and chemical stimuli. Activity of the 19 kD protein is similar to the cell death suppressing activity of the protein coded by the Bcl-2 protooncogene. Bcl-2 protein can functionally substitute for the E1B 19 kD protein during adenovirus infection and in transformation of primary cells with adenovirus E1A. Five different cDNA's for proteins, designated Nip1, Nip2, Nip3, Bip1A and Bip13 that specifically interact with the 19 kD protein were found. Mutational analysis of the interaction indicates that at least four of the proteins (Nip1, Nip2, Nip3 and Bip1A) associate with 19 kD protein at specific sites thereof. Homologous motifs are found on Bcl2. An additional protein, (Bip5), interacts with Bcl-2 but not with the 19K protein.

Abstract translation: 腺病毒E1B 19 kD蛋白可防止病毒感染引起的细胞死亡和某些外部物理和化学刺激。 19kD蛋白的活性与由Bcl-2原癌基因编码的蛋白质的细胞死亡抑制活性相似。 Bcl-2蛋白可以在腺病毒感染期间以及用腺病毒E1A转化原代细胞的功能上替代E1B19kD蛋白。 发现与9kD蛋白特异性相互作用的5种不同cDNA的cDNA，称为Nip1，Nip2，Nip3，Bip1A和Bip13。 相互作用的突变分析表明，至少有四种蛋白质（Nip1，Nip2，Nip3和Bip1A）在其特定位点与19kD蛋白质结合。 在Bcl2上发现同源基序。 另外一种蛋白质（Bip5）与Bcl-2相互作用，但不与19K蛋白质相互作用。