公开(公告)号：EP0099226A1

公开(公告)日：1984-01-25

申请号：EP83303903.5

申请日：1983-07-05

Applicant: ST. LOUIS UNIVERSITY

Inventor： Lambowitz, Alan M.

IPC: C12N15/00

CPC classification number: C12N15/80

Abstract: ( D A functional extrachromosomal element capable of replication in filamentous fungi is provided. The extrachromosomal element employs (1) a mitochondrial replicating element or (2) a lower organism replication sequence recognized by the fungus, in combination with foreign DNA to provide replication, transcription, and translation of foreign regulatory elements and genes. The extrachromosomal element is exemplified by a mitochondrial replicating system from Neurospora.
The cell strain strain E. coli HB101 containing the plasmid pALS-1-1 has been deposited at the A.T.C.C. on July 13, 1982, for patent purposes and given the designation ATCC 39157.
The cell strain E. coli HB101 containing the plasmid pALS-2 has been deposited at the A.T.C.C. on July 13, 1982, for patent purposes and given the designation ATCC 39158.

Abstract translation: 提供了能够在丝状真菌中复制的功能性染色体外元件。 染色体外元件使用（1）线粒体复制元件或（2）由真菌识别的下部生物复制序列，与外来DNA组合以提供外源调节元件和基因的复制，转录和翻译。 染色体外元件的例子是来自神经孢子虫的线粒体复制体系。 含有质粒pALS-1-1的细胞株菌株大肠杆菌HB101已经在A.T.C.C. 1982年7月13日，专利申请并命名为ATCC 39157.含有质粒pALS-2的细胞株大肠杆菌HB101已经存放在A.T.C.C. 于1982年7月13日授予专利，并命名为ATCC 39158。