公开(公告)号：JPS5822197B2

公开(公告)日：1983-05-07

申请号：JP2336875

申请日：1975-02-25

Applicant: STALEY MFG CO A E

Inventor： BULICH A

IPC: C12N9/44

公开(公告)号：JPS50117987A

公开(公告)日：1975-09-16

申请号：JP2336875

申请日：1975-02-25

Applicant: STALEY MFG CO A E

Inventor： BULICH A

IPC: C12N9/44

公开(公告)号：NL180333C

公开(公告)日：1987-02-02

申请号：NL7502253

申请日：1975-02-25

Applicant: STALEY MFG CO A E

Inventor： BULICH A

IPC: C12N9/44

Abstract: Improved pullulanase yields are achieved by incubating culture mediums containing pullulanase producing mutants of the Klebsiella genus. The mutants generally produce approximately equivalent amounts of extracellular and superficially bound pullulanase in an easily recoverable and usable form. Optimum pullulanase production is achieved when amylopectin is used as the sole carbohydrate source. Conventional pullulanase inducers such as maltose, maltotriose and/or pullulan repress the mutant strains capacity to produce pullulanase. The mutants are capable of elaborating pullulanase in a culture media containing dextrose as the sole carbohydrate source.

公开(公告)号：NL180333B

公开(公告)日：1986-09-01

申请号：NL7502253

申请日：1975-02-25

Applicant: STALEY MFG CO A E

Inventor： BULICH A

IPC: C12N9/44

Abstract: Improved pullulanase yields are achieved by incubating culture mediums containing pullulanase producing mutants of the Klebsiella genus. The mutants generally produce approximately equivalent amounts of extracellular and superficially bound pullulanase in an easily recoverable and usable form. Optimum pullulanase production is achieved when amylopectin is used as the sole carbohydrate source. Conventional pullulanase inducers such as maltose, maltotriose and/or pullulan repress the mutant strains capacity to produce pullulanase. The mutants are capable of elaborating pullulanase in a culture media containing dextrose as the sole carbohydrate source.

公开(公告)号：AR213270A1

公开(公告)日：1979-01-15

申请号：AR25774375

申请日：1975-02-24

Applicant: STALEY MFG CO A E

Inventor： BULICH A

IPC: C12N9/44 ,  C12D13/10

Abstract: Improved pullulanase yields are achieved by incubating culture mediums containing pullulanase producing mutants of the Klebsiella genus. The mutants generally produce approximately equivalent amounts of extracellular and superficially bound pullulanase in an easily recoverable and usable form. Optimum pullulanase production is achieved when amylopectin is used as the sole carbohydrate source. Conventional pullulanase inducers such as maltose, maltotriose and/or pullulan repress the mutant strains capacity to produce pullulanase. The mutants are capable of elaborating pullulanase in a culture media containing dextrose as the sole carbohydrate source.

公开(公告)号：BR7501110A

公开(公告)日：1975-12-02

申请号：BR7501110

申请日：1975-02-24

Applicant: STALEY MFG CO A E

Inventor： BULICH A

IPC: C12N9/44 ,  C12D3/10

Abstract: Improved pullulanase yields are achieved by incubating culture mediums containing pullulanase producing mutants of the Klebsiella genus. The mutants generally produce approximately equivalent amounts of extracellular and superficially bound pullulanase in an easily recoverable and usable form. Optimum pullulanase production is achieved when amylopectin is used as the sole carbohydrate source. Conventional pullulanase inducers such as maltose, maltotriose and/or pullulan repress the mutant strains capacity to produce pullulanase. The mutants are capable of elaborating pullulanase in a culture media containing dextrose as the sole carbohydrate source.

公开(公告)号：BE825317A

公开(公告)日：1975-08-07

申请号：BE153179

申请日：1975-02-07

Applicant: STALEY MFG CO A E

Inventor： BULICH A

IPC: C12N9/44 ,  C12D

Abstract: Improved pullulanase yields are achieved by incubating culture mediums containing pullulanase producing mutants of the Klebsiella genus. The mutants generally produce approximately equivalent amounts of extracellular and superficially bound pullulanase in an easily recoverable and usable form. Optimum pullulanase production is achieved when amylopectin is used as the sole carbohydrate source. Conventional pullulanase inducers such as maltose, maltotriose and/or pullulan repress the mutant strains capacity to produce pullulanase. The mutants are capable of elaborating pullulanase in a culture media containing dextrose as the sole carbohydrate source.

公开(公告)号：DE2507787C2

公开(公告)日：1985-11-28

申请号：DE2507787

申请日：1975-02-22

Applicant: STALEY MFG CO A E

Inventor： BULICH A

IPC: C12N9/44

Abstract: Improved pullulanase yields are achieved by incubating culture mediums containing pullulanase producing mutants of the Klebsiella genus. The mutants generally produce approximately equivalent amounts of extracellular and superficially bound pullulanase in an easily recoverable and usable form. Optimum pullulanase production is achieved when amylopectin is used as the sole carbohydrate source. Conventional pullulanase inducers such as maltose, maltotriose and/or pullulan repress the mutant strains capacity to produce pullulanase. The mutants are capable of elaborating pullulanase in a culture media containing dextrose as the sole carbohydrate source.

公开(公告)号：GB1499340A

公开(公告)日：1978-02-01

申请号：GB653975

申请日：1975-02-17

Applicant: STALEY MFG CO A E

Inventor： BULICH A

IPC: C12N9/44 ,  C07G7/028

Abstract: 1499340 Making pullulanase preparations A E STALEY MFG CO 17 Feb 1975 [25 Feb 1974] 6539/75 Heading C3H A method for producing pullulanase by inoculating an aqueous nutrient medium containing assimilable carbon and nitrogen sources with an aerobic, pullulanase-producing microbial organism to provide a culture thereof, incubating the culture under aerobic conditions conducive to the production of pullulanase and thereby providing production culture media thereof, and thereafter recovering a pullulanase preparation from the production culture media, is characterized in that the microbial organism (a) elaborates at least three times more pullulanase when amylopectin of a dextrose equivalent of less than 2À0 is employed as the sole carbohydrate source comparative to pullulanase elaboration in a nutrient medium wherein the major carbohydrate, on an equivalent carbohydrate weight basis, is a carbohydrate source member selected from maltose, dextrose, lactose and pullanan; (b) elaborates pullulanase at a ratio of extra-cellular pullulanase to superficially bound cell pullulanase of from 2 : 3 to less than 7 : 3whentheorganismiselaboratedinanutrient medium containing amylopectin as the sole carbohydrate source; (c) elaborates pullulanase in a nutrient medium which contains dextrose as a sole carbohydrate source; and wherein the organism is incubated in a nutrient medium for a period of time sufficient to provide a production culture medium which contains at least 350 units of pullulanase for each millilitre of production culture medium, the production culture medium containing a ratio of extracellular pullulanase to superficially bound pullulanase of from 2 : 3 to less than 7 : 3. Preferably the micro-organism used is an artificially induced Klebsiella mutant preparation containing Klebsiella pneumoniae NRRL-B-5780, Klebsiella pneumoniae NRRL-B-5783 and Klebsiella pneumoniae NRRL-B-5784.

公开(公告)号：FR2273065B1

公开(公告)日：1977-11-18

申请号：FR7505752

申请日：1975-02-25

Applicant: STALEY MFG CO A E

Inventor： BULICH A

IPC: C12N9/44 ,  C12D13/10 ,  C12D13/04

Abstract: Improved pullulanase yields are achieved by incubating culture mediums containing pullulanase producing mutants of the Klebsiella genus. The mutants generally produce approximately equivalent amounts of extracellular and superficially bound pullulanase in an easily recoverable and usable form. Optimum pullulanase production is achieved when amylopectin is used as the sole carbohydrate source. Conventional pullulanase inducers such as maltose, maltotriose and/or pullulan repress the mutant strains capacity to produce pullulanase. The mutants are capable of elaborating pullulanase in a culture media containing dextrose as the sole carbohydrate source.