公开(公告)号：WO2013063366A3

公开(公告)日：2013-07-11

申请号：PCT/US2012062073

申请日：2012-10-26

Applicant: STC UNM

Inventor： HJELLE BRIAN L ,  PEABODY DAVID S ,  CHACKERIAN BRYCE

IPC: G01N33/68 ,  C12Q1/68 ,  G01N33/569

CPC classification number: G01N33/6878 ,  G01N33/56983 ,  G01N33/6845

Abstract: The invention is directed to methods of screening immunogenic viral like particles and related immunogenic compositions and diagnostic techniques. In one embodiment, the invention provides methods of screening immunogenic viral like particles containing peptides corresponding to epitope regions of a wide variety of pathogens, including viruses, bacteria, parasites, and microbes. Non-infectious antigens and allergens of interest can also be screened as described herein. Immunization, therapeutic and diagnostic applications are also described for the compositions and methods according to the invention. In another embodiment, the invention provides novel methods of identifying a cryptic neutralizing epitope and related vaccines, constructs, and libraries. In some embodiments, these methods use high-throughput formats that are facilitated by in silica or in vitro steps.

Abstract translation: 本发明涉及筛选免疫原性病毒样颗粒和相关的免疫原性组合物和诊断技术的方法。 在一个实施方案中，本发明提供筛选含有对应于多种病原体（包括病毒，细菌，寄生虫和微生物）的表位区域的肽的免疫原性病毒样颗粒的方法。 还可以如本文所述筛选感兴趣的非传染性抗原和过敏原。 还描述了根据本发明的组合物和方法的免疫，治疗和诊断应用。 在另一个实施方案中，本发明提供鉴定隐性中和表位和相关疫苗，构建体和文库的新方法。 在一些实施方案中，这些方法使用通过二氧化硅或体外步骤促进的高通量形式。