公开(公告)号：US20180245049A1

公开(公告)日：2018-08-30

申请号：US15757218

申请日：2016-09-05

Applicant: STICHTING KATHOLIEKE UNIVERSITEIT

Inventor： Wouter Franciscus Joannes FEITZ ,  Egbert OOSTERWIJK ,  Silvia Maria MIHAILA ,  Alan Edward ROWAN

IPC: C12N5/0775 ,  C12N5/00

CPC classification number: C12N5/0663 ,  C08L71/02 ,  C12N5/0062 ,  C12N2506/1346 ,  C12N2513/00 ,  C12N2527/00 ,  C12N2533/30 ,  C12N2533/32 ,  C12N2533/40

Abstract: A cell culture including a cell culturing medium for growing stem cells, a three-dimensional (3D) cell growth matrix and stem cells, wherein the cell culture has a critical stress σc of 2-30 Pa, wherein the critical stress is a stress which marks an onset of strain stiffening and wherein the cell culture has a storage modulus G′ measured at 37° C. of 50-1000 Pa.

公开(公告)号：US20160137977A1

公开(公告)日：2016-05-19

申请号：US14905579

申请日：2014-07-16

Applicant: STICHTING KATHOLIEKE UNIVERSITEIT

Inventor： Alan Edward ROWAN ,  Zaskia Hillet EKSTEEN AKEROYD ,  Christopher WILSON ,  Petrus Johannes GEUTJES ,  Wouter F.J. FEITZ ,  Egbert OOSTERWIJK

IPC: C12N5/071 ,  C07K5/062

CPC classification number: C12N5/069 ,  C07K5/06026 ,  C08G65/325 ,  C08G65/33324 ,  C08G69/10 ,  C08G69/40 ,  C12N5/0691 ,  C12N2502/28 ,  C12N2533/50

Abstract: A process for making an oligo(alkylene glycol) functionalized co-polyisocyanopeptide, wherein the process includes the steps of: i) copolymerizing a first comonomer of an oligo(alkylene glycol) functionalized isocyanopeptide grafted with a linking group and a second comonomer of a non-grafted oligo(alkylene glycol) functionalized isocyanopeptide, wherein the molar ratio between the first comonomer and the second comonomer is 1:500 and 1:30 and ii) adding a reactant of a spacer unit and a cell adhesion factor to the copolymer obtained by step i), wherein the spacer unit is represented by general formula A-L-B, wherein the linking group and group A are chosen to react and form a first coupling and the cell adhesion factor and group B are chosen to react and form a second coupling, wherein the first coupling and the second coupling are independently selected from the group consisting of alkyne-azide coupling, dibenzocyclooctyne-azide coupling, oxanorbornmadiene-based-azide couplings, vinylsulphone-thiol coupling, maleimide-thiol coupling, methyl methacrylate-thiol coupling, ether coupling, thioether coupling, biotin-strepavidin coupling, amine-carboxylic acid resulting in amides linkages, alcohol-carboxylic acid coupling resulting in esters linkages and NHS-Ester (N-Hydroxysuccinimide ester)-amine coupling and wherein group L is a linear chain segment having 10-60 bonds between atoms selected from C, N, O and S in the main chain.

Abstract translation: 制备低聚（亚烷基二醇）官能化的共聚异氰尿苷的方法，其中该方法包括以下步骤：i）将接枝有连接基团的寡（亚烷基二醇）官能化异氰尿酸的第一共聚单体与非共轭聚异构体的第二共聚单体共聚， 接枝的寡聚（亚烷基二醇）官能化的异氰尿苷，其中第一共聚单体和第二共聚单体之间的摩尔比为1:500和1:30，和ii）将间隔单元的反应物和细胞粘附因子加入到由 步骤i），其中所述间隔单元由通式ALB表示，其中选择连接基团和基团A进行反应并形成第一偶联，并且选择细胞粘附因子和组B反应并形成第二偶联，其中 第一耦合和第二耦合独立地选自由炔 - 叠氮偶合，二苯并环辛烯 - 叠氮偶合，氧基雄烯二烯基叠氮化物偶联，viny 砜硫醇偶联，马来酰亚胺 - 硫醇偶联，甲基丙烯酸甲酯 - 硫醇偶联，醚偶联，硫醚偶联，生物素 - 链霉亲和素偶联，胺 - 羧酸引起酰胺键，产生酯键的醇 ​​- 羧酸偶联和NHS-酯（ N-羟基琥珀酰亚胺酯） - 胺偶联，其中基团L是在主链中选自C，N，O和S的原子之间具有10-60个键的直链链段。