公开(公告)号：US20180245049A1

公开(公告)日：2018-08-30

申请号：US15757218

申请日：2016-09-05

Applicant: STICHTING KATHOLIEKE UNIVERSITEIT

Inventor： Wouter Franciscus Joannes FEITZ ,  Egbert OOSTERWIJK ,  Silvia Maria MIHAILA ,  Alan Edward ROWAN

IPC: C12N5/0775 ,  C12N5/00

CPC classification number: C12N5/0663 ,  C08L71/02 ,  C12N5/0062 ,  C12N2506/1346 ,  C12N2513/00 ,  C12N2527/00 ,  C12N2533/30 ,  C12N2533/32 ,  C12N2533/40

Abstract: A cell culture including a cell culturing medium for growing stem cells, a three-dimensional (3D) cell growth matrix and stem cells, wherein the cell culture has a critical stress σc of 2-30 Pa, wherein the critical stress is a stress which marks an onset of strain stiffening and wherein the cell culture has a storage modulus G′ measured at 37° C. of 50-1000 Pa.