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    METHOD FOR SELECTING GENETICALLY TRANSFORMED CELLS
    1.
    发明专利
    METHOD FOR SELECTING GENETICALLY TRANSFORMED CELLS 未知

    公开(公告)号：CA2466088A1

    公开(公告)日：2003-05-15

    申请号：CA2466088

    申请日：2002-11-06

    Applicant: STICHTING TECH WETENSCHAPP

    Inventor： SCHLUEPMANN HENRIETTE ,  SMEEKENS JOSEPHUS CHRISTIANUS

    IPC: A01H5/00 ,  C12N5/10 ,  C12N9/24 ,  C12N15/09 ,  C12N15/82 ,  C12Q1/04 ,  C12N5/04

    Abstract: An environmental friendly and non toxic method for selection of transformed cells from a population consisting of transformed and non-trasnsformed cells . The method comprises the following steps a) introducing into a cell at least one desired nucleotide sequence and at least one selection-nucleotide sequen ce to obtain a genetically transformed cell, wherein the selection-nucleotide sequence comprises a region which codes for a protein involved in the metabolizing of trehalose; b) placing a population with transformed and non- transformed cells into contact with trehalose and/or derivative thereof; and c) selecting the transformed cells from the population on the basis of the capacity of the transformed cells to metabolize the trehalose and/or derivative.

    Method for selecting genetically transformed cells.
    2.
    发明专利
    Method for selecting genetically transformed cells. 未知

    公开(公告)号：ZA200403307B

    公开(公告)日：2005-01-24

    申请号：ZA200403307

    申请日：2004-04-30

    Applicant: STICHTING TECH WETENSCHAPP

    Inventor： SMEEKENS JOSEPHUS CHRISTIANUS ,  SCHLUEPMANN HENRIETTE

    IPC: C12N5/10 ,  C12N9/24 ,  A01H5/00 ,  C12N15/09 ,  C12N15/82 ,  C12Q1/04 ,  C12N ,  A01H

    Abstract: An environmental friendly and non toxic method for selection of transformed cells from a population consisting of transformed and non-transformed cells. The method comprises the following steps a) introducing into a cell at least one desired nucleotide sequence and at least one selection-nucleotide sequence to obtain a genetically transformed cell, wherein the selection-nucleotide sequence comprises a region which codes for a protein involved in the metabolizing of trehalose; b) placing a population with transformed and non-transformed cells into contact with trehalose and/or derivative thereof; and c) selecting the transformed cells from the population on the basis of the capacity of the transformed cells to metabolize the trehalose and/or derivative.

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