公开(公告)号：WO2014196854A1

公开(公告)日：2014-12-11

申请号：PCT/NL2014/050351

申请日：2014-06-02

Applicant: STICHTING VU-VUMC

Inventor： WUITE, Gijs Jan Lodewijk ,  PETERMAN, Erwin Johannes Gerard ,  HELLER, Iddo ,  SITTERS, Gerrit ,  CANDELLI, Andrea ,  HELL, Stefan Walter

IPC: G01N21/64 ,  G02B21/00 ,  G02B21/16 ,  G02B21/32 ,  B01L3/00 ,  C12Q1/68

CPC classification number: G01N21/6428 ,  B01L3/502761 ,  B01L2200/0663 ,  B01L2300/0877 ,  B01L2400/0454 ,  C12Q1/6816 ,  G01N21/6458 ,  G02B21/0048 ,  G02B21/0076 ,  G02B21/16 ,  G02B21/32 ,  C12Q2565/629

Abstract: The present disclosure concerns a method and system for imaging a molecular strand (MS). The method comprises providing a sample volume (SV) comprising the strand (MS); providing an excitation beam (EB) having an excitation focus (EF) in the sample volume (SV); scanning the excitation focus (EF) in the sample volume (SV) along a one dimensional scanning line (SL); trapping an end of the strand (MS) in the sample volume (SV) and extending the strand (MS) along a one-dimensional trapping line (LL) parallel to the scanning line (SL); aligning the trapping line (LL) to coincide with the scanning line (SL) to have the scanning excitation focus (EF) coincide with the strand (MS); and recording the fluorescence response (FR) as a function of a plurality of distinct scanning positions (X0) of the excitation focus (EF) along the scanning line (SL).

Abstract translation: 本公开涉及用于使分子链（MS）成像的方法和系统。 该方法包括提供包含链（MS）的样品体积（SV）; 提供在样本体积（SV）中具有激发焦点（EF）的激发束（EB）; 沿着一维扫描线（SL）扫描样品体积（SV）中的激发聚焦（EF）; 将样品体积（SV）中的股线（MS）的末端捕获并沿着平行于扫描线（SL）的一维捕获线（LL）延伸股线（MS）; 对准捕获线（LL）以与扫描线（SL）重合以使扫描激发聚焦（EF）与链（MS）重合; 以及沿着扫描线（SL）记录作为激发焦点（EF）的多个不同扫描位置（X0）的函数的荧光响应（FR）。

公开(公告)号：EP3004848A1

公开(公告)日：2016-04-13

申请号：EP14731835.6

申请日：2014-06-02

Applicant: Stichting VU-VUmc

Inventor： WUITE, Gijs Jan Lodewijk ,  PETERMAN, Erwin Johannes Gerard ,  HELLER, Iddo ,  SITTERS, Gerrit ,  CANDELLI, Andrea ,  HELL, Stefan Walter

IPC: G01N21/64 ,  G02B21/00 ,  G02B21/16 ,  G02B21/32 ,  B01L3/00 ,  C12Q1/68

CPC classification number: G01N21/6428 ,  B01L3/502761 ,  B01L2200/0663 ,  B01L2300/0877 ,  B01L2400/0454 ,  C12Q1/6816 ,  G01N21/6458 ,  G02B21/0048 ,  G02B21/0076 ,  G02B21/16 ,  G02B21/32 ,  C12Q2565/629

Abstract: The present disclosure concerns a method and system for imaging a molecular strand (MS). The method comprises providing a sample volume (SV) comprising the strand (MS); providing an excitation beam (EB) having an excitation focus (EF) in the sample volume (SV); scanning the excitation focus (EF) in the sample volume (SV) along a one dimensional scanning line (SL); trapping an end of the strand (MS) in the sample volume (SV) and extending the strand (MS) along a one-dimensional trapping line (LL) parallel to the scanning line (SL); aligning the trapping line (LL) to coincide with the scanning line (SL) to have the scanning excitation focus (EF) coincide with the strand (MS); and recording the fluorescence response (FR) as a function of a plurality of distinct scanning positions (X0) of the excitation focus (EF) along the scanning line (SL).

Abstract translation: 本公开涉及用于对分子链（MS）进行成像的方法和系统。 该方法包括提供包含链（MS）的样品体积（SV）; 提供在样本体积（SV）中具有激发焦点（EF）的激发光束（EB）; 沿着一维扫描线（SL）扫描样品体积（SV）中的激发聚焦（EF）; 将样品体积（SV）中的股线（MS）的末端捕获并沿着平行于扫描线（SL）的一维捕获线（LL）延伸股线（MS）; 对准捕获线（LL）与扫描线（SL）重合以使扫描激发聚焦（EF）与链（MS）重合; 以及沿着扫描线（SL）记录作为激发焦点（EF）的多个不同扫描位置（X0）的函数的荧光响应（FR）。