公开(公告)号：WO2013051932A1

公开(公告)日：2013-04-11

申请号：PCT/NL2012/050690

申请日：2012-10-03

Applicant: VERENIGING VOOR CHRISTELIJK HOGER ONDERWIJS,WETENSCHAPPELIJK ONDERZOEK EN PATIËNTENZORG ,  STICHTING VU-VUMC

Inventor： LAURENS, Niels ,  SITTERS, Gerrit ,  WUITE, Gijs Jan Lodewijk ,  PETERMAN, Erwin Johannes Gerard

IPC: H05H3/04 ,  G02B21/32 ,  B82Y15/00

CPC classification number: G02B21/32 ,  B82Y35/00

Abstract: A molecular manipulation apparatus for investigating molecules attached on one side to a surface of a sample and on another side to a bead. The apparatus having a radiation system for providing a radiation beam of radiation with a radiation intensity to the sample and the surface of the sample is transmissive for the radiation. The apparatus is provided with a sample holder for holding the sample with its transmissive surface within the beam of radiation. The apparatus has a detector for detecting a position of the beads; and, a computer programmed for calculating a physical property of the molecules as a function of the position of the plurality of beads.

Abstract translation: 一种分子操作装置，用于研究一侧附着在样品表面上的分子，另一侧用于研究珠粒。 具有用于向样品和样品表面提供具有辐射强度的辐射辐射束的辐射系统的装置对于辐射是透射的。 该装置设置有用于将样品保持在其辐射束内的透射表面的样品保持器。 该装置具有用于检测珠的位置的检测器; 以及被编程用于根据多个珠的位置计算分子的物理性质的计算机。

公开(公告)号：WO2014200341A1

公开(公告)日：2014-12-18

申请号：PCT/NL2014/050377

申请日：2014-06-11

Applicant: STICHTING VU-VUMC

Inventor： PETERMAN, Erwin Johannes Gerard ,  WUITE, Gijs Jan Lodewijk ,  SITTERS, Gerrit ,  KAMSMA, Douwe

IPC: G02B21/32 ,  B82Y35/00

CPC classification number: C12Q1/6837 ,  G01N29/0681 ,  G02B21/32

Abstract: A molecular manipulation system for investigating molecules,having a sample holder constructed to hold a sample comprising a plurality of molecules attached on one side to a surface in the sample holder and on another side attached to a microbead of a plurality of microbeads. The system having; an acoustic wave generator to generate an acoustic wave exerting a force on the microbeads in the sample; anda detector device to detect a response of the plurality of microbeads in the sample on the force exerted by the acoustic wave to investigate the molecules attached to the microbeads.

Abstract translation: 一种用于调查分子的分子操纵系统，具有样品架，其被构造成将包含附着在一侧的多个分子的样品保持在样品保持器中的表面上，另一侧附着在多个微珠的微珠上。 系统有 声波发生器，用于产生在样品中的微珠上施加力的声波; 以及检测器装置，用于检测样品中的多个微珠对由声波施加的力的响应，以研究附着于微珠的分子。

公开(公告)号：WO2014196854A1

公开(公告)日：2014-12-11

申请号：PCT/NL2014/050351

申请日：2014-06-02

Applicant: STICHTING VU-VUMC

Inventor： WUITE, Gijs Jan Lodewijk ,  PETERMAN, Erwin Johannes Gerard ,  HELLER, Iddo ,  SITTERS, Gerrit ,  CANDELLI, Andrea ,  HELL, Stefan Walter

IPC: G01N21/64 ,  G02B21/00 ,  G02B21/16 ,  G02B21/32 ,  B01L3/00 ,  C12Q1/68

CPC classification number: G01N21/6428 ,  B01L3/502761 ,  B01L2200/0663 ,  B01L2300/0877 ,  B01L2400/0454 ,  C12Q1/6816 ,  G01N21/6458 ,  G02B21/0048 ,  G02B21/0076 ,  G02B21/16 ,  G02B21/32 ,  C12Q2565/629

Abstract: The present disclosure concerns a method and system for imaging a molecular strand (MS). The method comprises providing a sample volume (SV) comprising the strand (MS); providing an excitation beam (EB) having an excitation focus (EF) in the sample volume (SV); scanning the excitation focus (EF) in the sample volume (SV) along a one dimensional scanning line (SL); trapping an end of the strand (MS) in the sample volume (SV) and extending the strand (MS) along a one-dimensional trapping line (LL) parallel to the scanning line (SL); aligning the trapping line (LL) to coincide with the scanning line (SL) to have the scanning excitation focus (EF) coincide with the strand (MS); and recording the fluorescence response (FR) as a function of a plurality of distinct scanning positions (X0) of the excitation focus (EF) along the scanning line (SL).

Abstract translation: 本公开涉及用于使分子链（MS）成像的方法和系统。 该方法包括提供包含链（MS）的样品体积（SV）; 提供在样本体积（SV）中具有激发焦点（EF）的激发束（EB）; 沿着一维扫描线（SL）扫描样品体积（SV）中的激发聚焦（EF）; 将样品体积（SV）中的股线（MS）的末端捕获并沿着平行于扫描线（SL）的一维捕获线（LL）延伸股线（MS）; 对准捕获线（LL）以与扫描线（SL）重合以使扫描激发聚焦（EF）与链（MS）重合; 以及沿着扫描线（SL）记录作为激发焦点（EF）的多个不同扫描位置（X0）的函数的荧光响应（FR）。

公开(公告)号：EP3008506A1

公开(公告)日：2016-04-20

申请号：EP14737025.8

申请日：2014-06-11

Applicant: Stichting VU-VUmc

Inventor： PETERMAN, Erwin Johannes Gerard ,  WUITE, Gijs Jan Lodewijk ,  SITTERS, Gerrit ,  KAMSMA, Douwe

IPC: G02B21/32 ,  B82Y35/00

CPC classification number: C12Q1/6837 ,  G01N29/0681 ,  G02B21/32

Abstract: A molecular manipulation system for investigating molecules,having a sample holder constructed to hold a sample comprising a plurality of molecules attached on one side to a surface in the sample holder and on another side attached to a microbead of a plurality of microbeads. The system having; an acoustic wave generator to generate an acoustic wave exerting a force on the microbeads in the sample; anda detector device to detect a response of the plurality of microbeads in the sample on the force exerted by the acoustic wave to investigate the molecules attached to the microbeads.

公开(公告)号：EP3004848A1

公开(公告)日：2016-04-13

申请号：EP14731835.6

申请日：2014-06-02

Applicant: Stichting VU-VUmc

Inventor： WUITE, Gijs Jan Lodewijk ,  PETERMAN, Erwin Johannes Gerard ,  HELLER, Iddo ,  SITTERS, Gerrit ,  CANDELLI, Andrea ,  HELL, Stefan Walter

IPC: G01N21/64 ,  G02B21/00 ,  G02B21/16 ,  G02B21/32 ,  B01L3/00 ,  C12Q1/68

CPC classification number: G01N21/6428 ,  B01L3/502761 ,  B01L2200/0663 ,  B01L2300/0877 ,  B01L2400/0454 ,  C12Q1/6816 ,  G01N21/6458 ,  G02B21/0048 ,  G02B21/0076 ,  G02B21/16 ,  G02B21/32 ,  C12Q2565/629

Abstract: The present disclosure concerns a method and system for imaging a molecular strand (MS). The method comprises providing a sample volume (SV) comprising the strand (MS); providing an excitation beam (EB) having an excitation focus (EF) in the sample volume (SV); scanning the excitation focus (EF) in the sample volume (SV) along a one dimensional scanning line (SL); trapping an end of the strand (MS) in the sample volume (SV) and extending the strand (MS) along a one-dimensional trapping line (LL) parallel to the scanning line (SL); aligning the trapping line (LL) to coincide with the scanning line (SL) to have the scanning excitation focus (EF) coincide with the strand (MS); and recording the fluorescence response (FR) as a function of a plurality of distinct scanning positions (X0) of the excitation focus (EF) along the scanning line (SL).

Abstract translation: 本公开涉及用于对分子链（MS）进行成像的方法和系统。 该方法包括提供包含链（MS）的样品体积（SV）; 提供在样本体积（SV）中具有激发焦点（EF）的激发光束（EB）; 沿着一维扫描线（SL）扫描样品体积（SV）中的激发聚焦（EF）; 将样品体积（SV）中的股线（MS）的末端捕获并沿着平行于扫描线（SL）的一维捕获线（LL）延伸股线（MS）; 对准捕获线（LL）与扫描线（SL）重合以使扫描激发聚焦（EF）与链（MS）重合; 以及沿着扫描线（SL）记录作为激发焦点（EF）的多个不同扫描位置（X0）的函数的荧光响应（FR）。