公开(公告)号：WO2010072790A1

公开(公告)日：2010-07-01

申请号：PCT/EP2009/067805

申请日：2009-12-22

Applicant: STMICROELECTRONICS S.R.L. ,  ALESSI, Enrico ,  DI TRAPANI, Giovanni ,  LICCIARDELLO, Antonella

Inventor： ALESSI, Enrico ,  DI TRAPANI, Giovanni ,  LICCIARDELLO, Antonella

IPC: B01L3/00 ,  B01L7/00 ,  G01N35/00 ,  G01F23/00

CPC classification number: B01L7/52 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502753 ,  B01L2200/10 ,  B01L2200/143 ,  B01L2200/147 ,  B01L2300/0627 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/087 ,  B01L2300/1805 ,  G01N35/00663 ,  G01N35/00712

Abstract: The method for detecting the presence of liquids includes detecting an initial temperature (Tl) in a channel (9) accommodating a liquid; heating the channel for a predetermined test time (Δt2); detecting a test temperature (T2); determining a temperature variation (S) on the basis of the initial temperature, the test temperature, and the test time; and comparing the temperature variation with at least one threshold (Z). Before detecting an initial temperature, an ambient temperature is read, the channel is heated to the initial temperature, and is kept at the initial temperature for a time period.

Abstract translation: 用于检测液体存在的方法包括检测容纳液体的通道（9）中的初始温度（T1）; 加热通道预定测试时间（Δt2）; 检测测试温度（T2）; 基于初始温度，测试温度和测试时间确定温度变化（S）; 以及将温度变化与至少一个阈值（Z）进行比较。 在检测初始温度之前，读取环境温度，将通道加热至初始温度，并保持初始温度一段时间。

公开(公告)号：WO2010076189A1

公开(公告)日：2010-07-08

申请号：PCT/EP2009/067167

申请日：2009-12-15

Applicant: STMICROELECTRONICS S.R.L. ,  ALESSI, Enrico ,  RICCERI, Daniele

Inventor： ALESSI, Enrico ,  RICCERI, Daniele

IPC: C12Q1/68

CPC classification number: C12Q1/6837 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q2527/101 ,  C12Q2561/113 ,  C12Q2527/107

Abstract: A method for carrying out nucleic acid amplification, includes providing a reaction chamber (31), accommodating an array (36) of nucleic acid probes (37) at respective locations, for hybridizing to respective target nucleic acids; and introducing a solution (50) into the reaction chamber (31), wherein the solution (50) contains primers, capable of binding to target nucleic acids, nucleotides, nucleic acid extending enzymes and a sample including nucleic acids. The a structure of the nucleic acid probes (37) and of the primers so that a hybridization temperature (T H ) of the probes (37) is higher than an annealing temperature (T A ) of the primers, whereby hybridization and annealing take place in respective separate temperature ranges (R H , R A ).

Abstract translation: 一种用于进行核酸扩增的方法，包括提供在相应位置容纳核酸探针（37）的阵列（36）的反应室（31），用于与各自的靶核酸杂交; 以及将溶液（50）引入所述反应室（31）中，其中所述溶液（50）含有能够结合靶核酸，核苷酸，核酸延伸酶和包含核酸的样品的引物。 核酸探针（37）的结构和引物的结构使得探针（37）的杂交温度（TH）高于引物的退火温度（TA），由此在各自的条件下进行杂交和退火 分开的温度范围（RH，RA）。

公开(公告)号：EP2382324A1

公开(公告)日：2011-11-02

申请号：EP09799323.2

申请日：2009-12-15

Applicant: STMicroelectronics S.r.l.

Inventor： ALESSI, Enrico ,  RICCERI, Daniele

IPC: C12Q1/68

CPC classification number: C12Q1/6837 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q2527/101 ,  C12Q2561/113 ,  C12Q2527/107

Abstract: A method for carrying out nucleic acid amplification, includes providing a reaction chamber (31), accommodating an array (36) of nucleic acid probes (37) at respective locations; for hybridizing to respective target nucleic acids; and introducing a solution (50) into the reaction chamber (31), wherein the solution (50) contains primers, capable of binding to target nucleic acids, nucleotides, nucleic acid extending enzymes and a sample including nucleic acids. The structure of the nucleic acid probes (37) and of the primers is selected so that a hybridization temperature (TH) of the probes (37) is higher than an annealing temperature (TA) of the primers, whereby hybridization and annealing take place in respective separate (non-overlapping) temperature ranges (RH, RA).

公开(公告)号：EP2382045A1

公开(公告)日：2011-11-02

申请号：EP09799637.5

申请日：2009-12-22

Applicant: STMicroelectronics S.r.l.

Inventor： ALESSI, Enrico ,  DI TRAPANI, Giovanni ,  LICCIARDELLO, Antonella

IPC: B01L3/00 ,  B01L7/00 ,  G01N35/00 ,  G01F23/00

CPC classification number: B01L7/52 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502753 ,  B01L2200/10 ,  B01L2200/143 ,  B01L2200/147 ,  B01L2300/0627 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/087 ,  B01L2300/1805 ,  G01N35/00663 ,  G01N35/00712

Abstract: The method for detecting the presence of liquids includes detecting an initial temperature (Tl) in a channel (9) accommodating a liquid; heating the channel for a predetermined test time (Δt2); detecting a test temperature (T2); determining a temperature variation (S) on the basis of the initial temperature, the test temperature, and the test time; and comparing the temperature variation with at least one threshold (Z). Before detecting an initial temperature, an ambient temperature is read, the channel is heated to the initial temperature, and is kept at the initial temperature for a time period.