公开(公告)号：WO2010076189A1

公开(公告)日：2010-07-08

申请号：PCT/EP2009/067167

申请日：2009-12-15

Applicant: STMICROELECTRONICS S.R.L. ,  ALESSI, Enrico ,  RICCERI, Daniele

Inventor： ALESSI, Enrico ,  RICCERI, Daniele

IPC: C12Q1/68

CPC classification number: C12Q1/6837 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q2527/101 ,  C12Q2561/113 ,  C12Q2527/107

Abstract: A method for carrying out nucleic acid amplification, includes providing a reaction chamber (31), accommodating an array (36) of nucleic acid probes (37) at respective locations, for hybridizing to respective target nucleic acids; and introducing a solution (50) into the reaction chamber (31), wherein the solution (50) contains primers, capable of binding to target nucleic acids, nucleotides, nucleic acid extending enzymes and a sample including nucleic acids. The a structure of the nucleic acid probes (37) and of the primers so that a hybridization temperature (T H ) of the probes (37) is higher than an annealing temperature (T A ) of the primers, whereby hybridization and annealing take place in respective separate temperature ranges (R H , R A ).

Abstract translation: 一种用于进行核酸扩增的方法，包括提供在相应位置容纳核酸探针（37）的阵列（36）的反应室（31），用于与各自的靶核酸杂交; 以及将溶液（50）引入所述反应室（31）中，其中所述溶液（50）含有能够结合靶核酸，核苷酸，核酸延伸酶和包含核酸的样品的引物。 核酸探针（37）的结构和引物的结构使得探针（37）的杂交温度（TH）高于引物的退火温度（TA），由此在各自的条件下进行杂交和退火 分开的温度范围（RH，RA）。

公开(公告)号：EP2382324A1

公开(公告)日：2011-11-02

申请号：EP09799323.2

申请日：2009-12-15

Applicant: STMicroelectronics S.r.l.

Inventor： ALESSI, Enrico ,  RICCERI, Daniele

IPC: C12Q1/68

CPC classification number: C12Q1/6837 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q2527/101 ,  C12Q2561/113 ,  C12Q2527/107

Abstract: A method for carrying out nucleic acid amplification, includes providing a reaction chamber (31), accommodating an array (36) of nucleic acid probes (37) at respective locations; for hybridizing to respective target nucleic acids; and introducing a solution (50) into the reaction chamber (31), wherein the solution (50) contains primers, capable of binding to target nucleic acids, nucleotides, nucleic acid extending enzymes and a sample including nucleic acids. The structure of the nucleic acid probes (37) and of the primers is selected so that a hybridization temperature (TH) of the probes (37) is higher than an annealing temperature (TA) of the primers, whereby hybridization and annealing take place in respective separate (non-overlapping) temperature ranges (RH, RA).