公开(公告)号：US3819488A

公开(公告)日：1974-06-25

申请号：US16441571

申请日：1971-07-20

Applicant: TECHNICON INSTR

Inventor： RUSH R ,  LEON L ,  DE LEA A

IPC: C12Q1/32 ,  C12Q1/52 ,  G01N31/14

CPC classification number: C12Q1/52 ,  C12Q1/32

Abstract: Stable, lyophilized diagnostic compositions are disclosed effective in determining glutamic oxalic transaminase (GOT) and glutamic pyruvic transaminase (GPT) comprising malate dehydrogenase (for GOT) or lactate dehydrogenase (for GPT), a pyridine nucleotide and a buffer having a sodium content of less than about 600 meq./1. capable of regulating the pH of the composition in reconstituted form. Also disclosed are the aqueous reconstituted products, the method of determining the activity of GOT and GPT in a sample using the subject compositions and the process for making the disclosed diagnostic compositions.

Abstract translation: 公开了稳定的冻干诊断组合物，用于测定含有苹果酸脱氢酶（GOT）或乳酸脱氢酶（GPT）的谷氨酸转氨酶（GOT）和谷氨酸丙酮酸转氨酶（GPT），吡啶核苷酸和钠含量少的缓冲液 比约600meq./1。 能够以重构形式调节组合物的pH。 还公开了水性重构产物，使用主题组合物和制备所公开的诊断组合物的方法测定样品中GOT和GPT的活性的方法。