公开(公告)号：US10266814B2

公开(公告)日：2019-04-23

申请号：US15315484

申请日：2015-06-02

Applicant: THE BOARD OF REGENTS FOR OKLAHOMA STATE UNIVERSITY

Inventor： Andrew Mort ,  Anamika Ray ,  Sayali S. Saykhedkar ,  Rolf A. Prade

IPC: C12N9/02 ,  C12N9/42 ,  C12P19/02 ,  C12P19/14 ,  C12N9/24 ,  C12P19/00

Abstract: Compositions comprising glycosyl hydrolase enzymes are provided, as are methods for their use to depolymerize hemicellulose, cellulose, lignin and pectin in biomass in order to produce products such as simple sugars. The enzymes, isolated from Aspergillus nidulans and Phanerochaete chrysosporium, were characterized, and synergistic mixtures of the enzymes were produced and used to generate simple sugars from biomass without the need to pretreat the biomass before digestion. The enzyme blends generally comprise two or more enzymes, which may be from the same fungus or from two different fungi, and are used for efficient and cost effective complete degradation of lignocelluloses. Applications of this technology include biofuel production.