公开(公告)号：US20170198270A1

公开(公告)日：2017-07-13

申请号：US15315484

申请日：2015-06-02

Applicant: THE BOARD OF REGENTS FOR OKLAHOMA STATE UNIVERSITY

Inventor： ANDREW MORT ,  ANAMIKA RAY ,  SAYALI S. SAYKHEDKAR ,  ROLF A. PRADE

IPC: C12N9/24 ,  C12P19/02 ,  C12P19/14

CPC classification number: C12N9/2402 ,  C12N9/0083 ,  C12N9/24 ,  C12N9/2437 ,  C12P19/02 ,  C12P19/14 ,  C12P2203/00

Abstract: Compositions comprising glycosyl hydrolase enzymes are provided, as are methods for their use to depolymerize hemicellulose, cellulose, lignin and pectin in biomass in order to produce products such as simple sugars. The enzymes, isolated from Aspergillus nidulans and Phanerochaete chrysosporium, were characterized, and synergistic mixtures of the enzymes were produced and used to generate simple sugars from biomass without the need to pretreat the biomass before digestion. The enzyme blends generally comprise two or more enzymes, which may be from the same fungus or from two different fungi, and are used for efficient and cost effective complete degradation of lignocelluloses. Applications of this technology include biofuel production.

公开(公告)号：US20150004670A1

公开(公告)日：2015-01-01

申请号：US14318097

申请日：2014-06-27

Applicant: THE BOARD OF REGENTS FOR OKLAHOMA STATE UNIVERSITY

Inventor： MICHAEL MUELLER ,  MARK WILKINS ,  ROLF A. PRADE

IPC: C12N9/24

CPC classification number: C12N9/2482 ,  C12N1/14

Abstract: Methods of continuous production of a product of interest using a filamentous fungus are provided. The fungus is auxotrophic for a nutrient that is required for fungal growth, but not for fungal synthesis of the product of interest. Under growth limiting culture conditions, in which the nutrient is absent from the culture medium, the amount of fungus remains constant or nearly constant and nutrients are not used up for growth but instead are available for synthetic pathways which produce the product of interest.

Abstract translation: 提供使用丝状真菌连续生产感兴趣的产品的方法。 真菌对于真菌生长所需的营养素是营养缺陷型的，但不用于感兴趣的产品的真菌合成。 在生长限制性培养条件下，培养基中不存在营养物，真菌的数量保持恒定或几乎不变，并且营养物质不被用于生长，而是可用于产生感兴趣的产物的合成途径。