公开(公告)号：EP0563200A4

公开(公告)日：1994-02-02

申请号：EP92901880

申请日：1991-12-20

Applicant: THE COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION

Inventor： BRADY, COLIN ,  LEE, ELIZABETH ,  POGSON, BARRY, JAMES ,  ORR, GLENDA, ROBYN ,  SPEIRS, JAMES

IPC: A01H5/00 ,  C12N9/24 ,  C12N9/26 ,  C12N15/09 ,  C12N15/56

CPC classification number: C12Y302/01015 ,  C12N9/2402

Abstract: The present invention provides an isolated DNA sequence which encodes at least part of the tomato enzyme endopolygalacturonase PG1 β-subunit. This DNA sequence preferably encodes polypeptide the N-terminal amino acid sequence of which is: E-K-H-S-G-D-I-H-G-A-T-Y-S-D-K. In other preferred forms of the invention the DNA sequence encodes a polypeptide which includes one or both of the following amino acid sequences: D-A-N-D-I-E-A-N-T-Y-N-Y-G-Q-X-F-N-E-G; A-L-N-A-E-N-Q-V-R-I-K. The DNA sequence of the present invention may be used to produce genetically engineered tomato plants with modified riping characteristics.

Abstract translation: 本发明提供了分离的DNA序列，其编码至少部分番茄酶内切聚糖半乳糖醛酸酶PG1β-亚基。 该DNA序列优选编码N-末端氨基酸序列为E-K-H-S-G-D-I-H-G-A-T-Y-S-D-K的多肽。 在本发明的其它优选形式中，DNA序列编码包括以下氨基酸序列中的一个或两个的多肽：D-A-N-D-I-E-A-N-T-Y-N-Y-G-Q-X-F-N-E-G; A-L-N-A-E-N-Q-V-R-I-K。 本发明的DNA序列可用于生产具有改进的成膜特征的基因工程番茄植物。