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公开(公告)号：US20200332312A1
公开(公告)日：2020-10-22
申请号：US16843519
申请日：2020-04-08
Applicant: THE TEXAS A&M UNIVERSITY SYSTEM
Inventor： T. Erik MIRKOV , Kranthi Kiran MANDADI
IPC: C12N15/82 , C07K14/415
Abstract: The present disclosure relates, according to some embodiments, to pathogen resistant plants, compositions, organisms, systems, and methods. For example, a composition may comprise a heterologous peptide (e.g., a defensin peptide) and/or a nucleic acid (e.g., a defensin nucleic acid). A pathogen resistant plant may comprise, in some embodiments, a heterologous defensin peptide and/or an expressible nucleic acid encoding a heterologous defensin peptide.
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2.发明申请COMPOSITIONS, ORGANISMS, SYSTEMS, AND METHODS FOR EXPRESSING A GENE PRODUCT IN PLANTS 审中-公开用于在植物中表达基因产物的组合物，有机体，系统和方法公开(公告)号：US20140208462A1
公开(公告)日：2014-07-24
申请号：US14185684
申请日：2014-02-20
Applicant: THE TEXAS A&M UNIVERSITY SYSTEM
Inventor： Mona DAMAJ , T. Erik MIRKOV
IPC: C12N15/82
CPC classification number: C12N15/8216 , C12N9/2462 , C12N15/52 , C12N15/8222 , C12N15/8237 , C12N15/8241
Abstract: The present disclosure relates, in some embodiments, to compositions, organisms, systems, and methods for expressing a gene product in a plant using a expression control sequence (ECS) operable in monocots and/or dicots. For example, (i) an isolated nucleic acid may comprise an ECS (e.g., a sugarcane bacilliform virus promoter) and, optionally, an exogenous nucleic acid (ExNA) operably linked to the ECS; (ii) an expression vector may comprise an ECS; an ExNA; and, optionally, a 3′ termination sequence, wherein the ECS has promoter activity sufficient to express the ExNA in at least one monocot and at least one dicot; (iii) a microorganism, plant cell, or plant may comprise an isolated nucleic acid; (iv) a method for constitutively expressing an ExNA in a plant (e.g., a monocot and/or a dicot) may comprise, contacting an expression vector with the cytosol of a cell of the plant, wherein the expression vector comprises the ExNA and an ECS operable to drive expression of the ExNA; and/or (v) a method of directing constitutive expression of a nucleic acid in a plant (e.g., a monocot and/or a dicot) may comprise transforming the plant with an expression nucleic acid comprising an ECS, an ExNA, and a 3′ termination sequence.
Abstract translation: 在一些实施方案中，本公开涉及使用在单子叶植物和/或双子叶植物中可操作的表达调控序列（ECS）在植物中表达基因产物的组合物，生物，系统和方法。 例如，（i）分离的核酸可以包含可操作地连接到ECS的ECS（例如，甘蔗杆状病毒启动子）和任选的外源核酸（ExNA）; （ii）表达载体可以包含ECS; 一个ExNA; 和任选的3'终止序列，其中ECS具有足以在至少一个单子叶植物和至少一个双子叶植物中表达ExNA的启动子活性; （iii）微生物，植物细胞或植物可以包含分离的核酸; （iv）在植物（例如单子叶植入物和/或双子叶植物）中组成型表达ExNA的方法可以包括使表达载体与植物细胞的细胞溶质接触，其中表达载体包含ExNA和 ECS可以推动ExNA的表达; 和/或（v）指导核酸在植物（例如单子叶植物和/或双子叶植物）中的组成型表达的方法可包括用包含ECS，ExNA和3的表达核酸转化植物 '终止序列。
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3.发明申请COMPOSITIONS, ORGANISMS, SYSTEMS, AND METHODS FOR EXPRESSING A GENE PRODUCT IN PLANTS 审中-公开用于在植物中表达基因产物的组合物，有机体，系统和方法公开(公告)号：US20130247252A1
公开(公告)日：2013-09-19
申请号：US13800930
申请日：2013-03-13
Applicant: THE TEXAS A&M UNIVERSITY SYSTEM
Inventor： Mona B. DAMAJ , T. Erik MIRKOV , Terry L. THOMAS , Keerti S. RATHORE , Chandrakanth EMANI , Siva P. KUMPATLA
IPC: C12N15/113
CPC classification number: C12N15/113 , C07K14/415 , C12N9/1007 , C12N15/8226 , C12Y201/01
Abstract: The present disclosure relates, according to some embodiments, to compositions, organisms, systems, and methods for expressing a gene product in a plant (e.g., a monocot) using a promoter operable in one or more plant tissues and/or cells. In some embodiments, an isolated nucleic acid may comprise an expression control sequence having the sequence of nucleotides 1-4726 of SEQ ID NO: 1, wherein the expression control sequence has stem-regulated and/or defense-inducible promoter activity in at least one monocot (e.g., at least two monocots).
Abstract translation: 根据一些实施方案，本公开涉及使用可在一个或多个植物组织和/或细胞中可操作的启动子在植物（例如，单子叶植物）中表达基因产物的组合物，生物体系，系统和方法。 在一些实施方案中，分离的核酸可以包含具有SEQ ID NO：1的核苷酸1-4726的序列的表达调控序列，其中所述表达控制序列在至少一个中具有干细胞调节和/或防御诱导型启动子活性 单子叶植物（例如，至少两个单子叶植物）。
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Patent Agency Ranking
4.发明申请公开(公告)号：US20170137776A1
公开(公告)日：2017-05-18
申请号：US15353645
申请日：2016-11-16
Applicant: THE TEXAS A&M UNIVERSITY SYSTEM
Inventor： Kranthi K. MANDADI , Sonia C. IRIGOYEN , T. Erik MIRKOV
CPC classification number: C12N1/20 , A01H4/005 , A01H5/08 , A01H6/78 , C12N5/04 , C12N15/8241 , C12N2502/70 , C12Q1/025 , C12Q1/25 , C12Q1/689 , C12Q1/6895 , C12Q2600/158 , G01N2333/195 , G01N2333/415
Abstract: Numerous plant microbes, including the vascular-limited Candidatus spp.—causal agents of citrus greening and potato zebra chip diseases—are non-culturable. The present disclosure relates, according to some embodiments, to compositions, methods and systems for culturing such organisms. For example, the present disclosure relates to methods for culturing, propagating, and characterizing fastidious vascular-colonizing microbes using a hairy root system (e.g., in vitro, in planta). The present disclosure relates, in some embodiments, to methods for cultivating a fastidious plant microbe including: contacting a plant (e.g., a tomato plant, a potato plant, a citrus plant) colonized by a fastidious plant microbe (e.g., Xylella fastidiosa, Candidatus Liberibacter spp.) with a suspension of R. rhizogenes under conditions that permit induction of hairy roots colonized with the fastidious plant microbe, and propagating the colonized microbial hairy roots.
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5.发明申请MATERIALS, SYSTEMS, ORGANISMS, AND METHODS FOR ENHANCING ABIOTIC STRESS TOLERANCE, INCREASING BIOMASS, AND/OR ALTERING LIGNIN COMPOSITION 审中-公开材料，系统，有机体和增强生物量的方法，提高生物量和/或改变木质素组成公开(公告)号：US20170002374A1
公开(公告)日：2017-01-05
申请号：US15267129
申请日：2016-09-15
Applicant: THE TEXAS A&M UNIVERSITY SYSTEM
Inventor： T. Erik MIRKOV , Getu BEYENE , Mona DAMAJ
IPC: C12N15/82 , C07K14/415
CPC classification number: C12N15/8273 , C07K14/415 , C12N15/8218 , C12N15/8222 , C12N15/8237 , C12N15/8246 , C12N15/8255 , C12N15/8261 , C12N15/8297 , Y02A40/146
Abstract: The present disclosure relates, in some embodiments, to materials, systems, organisms, and methods for enhancing abiotic stress tolerance (e.g., cold, salinity, drought, wind), increasing biomass, and/or altering lignin composition in plants. For example, enhancing abiotic stress tolerance may be achieved using a plant-specific family of transcription factors is APETALA2 (AP2), that includes c-repeat binding factor (e.g., CBF1, CBF3) and AP37 nucleic acids and/or polypeptides. In some embodiments, increasing biomass may be achieved by altering expression of gibberellin oxidases (e.g., GA3ox3/GA2ox4) nucleic acids and/or polypeptides. Altering lignin composition may be achieved by suppression of stem-thickening in pith (e.g., STP1) nucleic acids and/or polypeptides.
Abstract translation: 在一些实施方案中，本公开涉及用于增强非生物胁迫耐受性（例如，冷，盐度，干旱，风），增加生物质和/或改变植物中木质素组成的材料，系统，生物体和方法。 例如，可以使用植物特异性转录因子家族APETALA2（AP2）（其包括c重复结合因子（例如CBF1，CBF3）和AP37核酸和/或多肽）来实现增强非生物胁迫耐受性。 在一些实施方案中，可通过改变赤霉素氧化酶（例如GA3ox3 / GA2ox4）核酸和/或多肽的表达来实现增加的生物量。 改变木质素组合物可以通过抑制髓（例如STP1）核酸和/或多肽中的茎增稠来实现。
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6.发明申请公开(公告)号：US20130205443A1
公开(公告)日：2013-08-08
申请号：US13751936
申请日：2013-01-28
Applicant: THE TEXAS A&M UNIVERSITY SYSTEM
Inventor： T. Erik MIRKOV , Javier GONZALEZ-RAMOS
IPC: C12N15/82
CPC classification number: A01G2/30 , A01N65/00 , A01N65/08 , C07K14/415 , C12N15/8279 , C12N15/8281
Abstract: The present disclosure relates, according to some embodiments, to pathogen resistant citrus compositions, organisms, systems, and methods. For example, a composition may comprise a peptide (e.g., a defensin peptide) and/or a nucleic acid (e.g., a defensin nucleic acid). A pathogen resistant citrus plant may comprise, in some embodiments, a defensin peptide and/or an expressable nucleic acid encoding a defensin peptide.
Abstract translation: 根据一些实施方案，本公开涉及抗病原体的柑橘组合物，生物，系统和方法。 例如，组合物可以包含肽（例如防御素肽）和/或核酸（例如防御素核酸）。 在一些实施方案中，抗病原体的柑橘植物可以包含防御素肽和/或编码防御素肽的可表达核酸。
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7.发明申请COMPOSITIONS, ORGANISMS, SYSTEMS, AND METHODS FOR EXPRESSING A GENE PRODUCT IN PLANTS 有权用于在植物中表达基因产物的组合物，有机体，系统和方法公开(公告)号：US20150259697A1
公开(公告)日：2015-09-17
申请号：US14644091
申请日：2015-03-10
Applicant: THE TEXAS A&M UNIVERSITY SYSTEM
Inventor： Mona B. DAMAJ , T. Erik MIRKOV
CPC classification number: C12N15/8226 , C12N9/2402 , C12Y302/01031
Abstract: The present disclosure relates, according to some embodiments, to compositions, organisms, systems, and methods for expressing a gene product in a plant (e.g., a monocot) using a promoter operable in one or more plant tissues and/or cells. In some embodiments, an artificial nucleic acid may comprise an expression control sequence having the sequence selected from the sequence of nucleotides 1-4710 of SEQ ID NO: 1 and the sequence of nucleotides 1137-4710 of SEQ ID NO: 1, wherein the expression control sequence has stem-regulated promoter activity in at least one monocot (e.g., at least two monocots).
Abstract translation: 根据一些实施方案，本公开涉及使用可在一个或多个植物组织和/或细胞中可操作的启动子在植物（例如，单子叶植物）中表达基因产物的组合物，生物，系统和方法。 在一些实施方案中，人造核酸可以包含具有选自SEQ ID NO：1的核苷酸1-4710的序列和SEQ ID NO：1的核苷酸1137-4710的序列的表达调控序列，其中表达 控制序列在至少一个单子叶植物（例如，至少两个单子叶植物）中具有茎调节的启动子活性。
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8.发明申请公开(公告)号：US20140109472A1
公开(公告)日：2014-04-24
申请号：US14139791
申请日：2013-12-23
Applicant: THE TEXAS A&M UNIVERSITY SYSTEM
Inventor： T. Erik MIRKOV , Javier GONZALEZ-RAMOS
CPC classification number: C12N15/8279 , A01G2/30 , A01N65/00 , A01N65/08 , C07K14/415 , C12N15/8281
Abstract: The present disclosure relates, according to some embodiments, to pathogen resistant citrus compositions, organisms, systems, and methods. For example, a composition may comprise a peptide (e.g., a defensin peptide) and/or a nucleic acid (e.g., a defensin nucleic acid). A pathogen resistant citrus plant may comprise, in some embodiments, a defensin peptide and/or an expressable nucleic acid encoding a defensin peptide.
Abstract translation: 根据一些实施方案，本公开涉及抗病原体的柑橘组合物，生物，系统和方法。 例如，组合物可以包含肽（例如防御素肽）和/或核酸（例如防御素核酸）。 在一些实施方案中，抗病原体的柑橘植物可以包含防御素肽和/或编码防御素肽的可表达核酸。
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