公开(公告)号：JPH09257742A

公开(公告)日：1997-10-03

申请号：JP6295796

申请日：1996-03-19

Applicant: TOA ELECTRONICS

Inventor： CHO TETSUO ,  ANZAI JUNICHI ,  MOTOHASHI RYOICHI

IPC: G01N27/327 ,  C12M1/40 ,  C12Q1/26 ,  G01N27/416

Abstract: PROBLEM TO BE SOLVED: To enhance the selectivity and response to alcohol of an alcohol sensor and to stably use the sensor over a long period of time by providing a multiple layer film having avidin and biotin labelled alcohol oxidase alternately laminated thereto on the surface of a platinum electrode. SOLUTION: This alcohol sensor is constituted by providing a multiple layer film having avidin 2 and biotin labelled alcohol oxidase 3 alternately laminated thereto on the surface of a platinum electrode 1. That is, biotin 3b is added to alcohol oxidase 3a to form biotin labelled alcohol oxidase 3 and an avidin- biotin bong is utilized to form a protein layer of avidin-biotin containing alcohol oxidase 3a on the surface of the platinum electrode 1. This mechanism is based on that avidin has four bonding regions with biotin 3b and some biotins 3b are added to alcohol oxidase 3a. This sensor has a simple structure utilizing alcohol oxidase and is practical and has high reliability.