公开(公告)号：JPS5824852A

公开(公告)日：1983-02-14

申请号：JP12340181

申请日：1981-08-06

Applicant: TOA ELECTRONICS

Inventor： MOTOHASHI RIYOUICHI ,  YAMASATO MASAHARU

IPC: G01N27/30

Abstract: PURPOSE:To prevent blocking a liquid junction of an outer tube as a function of a single or a double junction type comparing electrode is maintained, by mounting an ion selective hardening material at an outer tube so that it makes contact with inner liquids at the inside and the outside of the outer tube. CONSTITUTION:A comparing electrode generates a steady potential by the aid of an inner electrode 3 and inner liquids 8 and 10, and is electrically connected to a sample liquid with the inner liquids 8 and 10. The inner liquids 8 and 10 at the inside and the outside of an outer tube 1 are electrically connected through an ion selecting hardening material 13 which consists of 5-50 pts.wt. epoxy resin, 5-25 pts.wt. dibenzol sorbitol or a derivative thereof, and remaining pts.wt. exchange liquid, and in which gel by the dibenzol sorbitol of the ion excange liquid or the derivative thereof is evenly dispersed and held in an epoxy resin hardening material.

公开(公告)号：JPS5824853A

公开(公告)日：1983-02-14

申请号：JP12340281

申请日：1981-08-06

Applicant: TOA ELECTRONICS

Inventor： MOTOHASHI RIYOUICHI ,  YAMASATO MASAHARU

IPC: G01N27/30

Abstract: PURPOSE:To prevent blocking of a liquid junction, by a method wherein, after an electrode lead-in wire covered with an insulating material is inserted into inner liquid through one end of an outer tube, the insulating material at the end part of the electrode lead-in wire in the inner liquid is unsheathed for exposure, the exposed part is surrounded with an ion selective hardening material. CONSTITUTION:A liquid junction 6 is formed in an outer tube 1 which is filled with conductive inner liquid 8, and after an electrode lead-in wire 4 covered with an insulating material is inserted into the inner liquid 8 through the one end of the outer tube 1, the insulating material at the one end of the electrode lead-in wire 4 in the inner liquid 8 is unsheathed for exposure. The exposed part is water tightly surrounded with an ion selective hardening material 13 in which gel by dibenzal sorbitol of ion exchange liquid or a derivative thereof is evenly dispersed and held in an epoxy resin hardening material consisting of 5-50 pts.wt. epoxy resin hardening material consisting 5-50 pts.wt. epoxy resin containing a hardener, 5-25 pts.wt. dibenzal sorbitol or a derivative thereof, and remaining pts.wt. ion exchange liquid.

公开(公告)号：JPS5666749A

公开(公告)日：1981-06-05

申请号：JP14268979

申请日：1979-11-02

Applicant: KYOWA HAKKO KOGYO KK ,  TOA ELECTRONICS

Inventor： SAKADO KUNIAKI ,  TANAKA HISAO ,  MOTOHASHI RIYOUICHI

IPC: C12M1/34 ,  G01N27/416

Abstract: PURPOSE:To simply and quickly measure the activity of animal/botanical tissue, by contacting the electrode or constant potential device to culture solution and measuring the current or voltage produced where animal/botanical tissue reacts with the electrode or constant potential device. CONSTITUTION:Electrodes 9, 10 incorporate and anode 3 and an anode solution 8 and have a connection lead 4 to contact with external liquid. One electrode 10 in which the platinum electrode 5 exposed is covered with an animal/botanical tissue opaque membrane 6 is an electrode to measure the electrode active substance other than the animal/botanical tissue present in fermentation liquid. The fermentation liquid of animal/botanical tissue or electrode active substance is in contact with the cathode 5 of the electrode 9 to produce a current or voltage. On the other hand, the electrode active substance only in also in contact with the cathode 5 of the electrode 10 to produce a current or potential. The difference of currents or potentials from two electrodes 9 and 10 very much corresponds to the number of cells and activity of animal/botanical tissue measured separately. Accordingly, the activity of animal/bontanical tissue can be measured.

公开(公告)号：JPS5666748A

公开(公告)日：1981-06-05

申请号：JP14269079

申请日：1979-11-02

Applicant: KYOWA HAKKO KOGYO KK ,  TOA ELECTRONICS

Inventor： SAKADO KUNIAKI ,  TANAKA HISAO ,  MOTOHASHI RIYOUICHI

IPC: G01N27/416 ,  C12Q1/00 ,  G01N27/30 ,  G01N27/327 ,  G01N33/48

Abstract: PURPOSE:To measure the biological activity simply and quickly, by contacting both the devices in which one set of the device is coated with biological constituent opaque membrane for the cathode among two sets of constant potential devices and giving a specified potential. CONSTITUTION:The electrodes 2, 3 of the constant potential devices provided in a tank 1 have two electrodes 4, 5, and one electrode of one constant potential device is covered with a membrane 6 of microbe, biological or botanical constituent opaque nature. Leads are connected to a device 7 which gives a given potential (electrolyte) from the respective electrode, and to a recorder 8. The electrode active substance in microbe (bacterium) and fermentation liquid (formic acid, hydrogen and coferment) is in contact with the cathode of the electrode 2 to produce currents and voltages. On the other hand, only the electrode active substance is also in contact with the electrode 3 of the constant potential devices to produce currents and voltages. The difference of currents and voltages of the two constant potential devices is an electric signal relating to the microbe activity, which is used for the measurement of activity of animal/botanical cells.

公开(公告)号：JPS57172242A

公开(公告)日：1982-10-23

申请号：JP5759281

申请日：1981-04-16

Applicant: TOA ELECTRONICS

Inventor： MOTOHASHI RIYOUICHI ,  AIKAWA KATSUAKI

IPC: G01N27/416 ,  C12Q1/26 ,  C12Q1/54

Abstract: PURPOSE:To determine the concentration of a substrate, by adding catalase to oxidoreductase and measuring the difference of hydrogen peroxide produced by the action of oxidoreductase and hydrogen peroxide consumed by the action of catalase electrochemically. CONSTITUTION:A dilution solution admixed with potassium chloride, glucose oxidase and catalase to a phosphate buffer solution, is filled in a tank 7 and is sent to a flow cell 2 by a pump 6. A sample solution containing beta-D glucose injected from a sample injection opening 5 and the dilution solution, is mixed by a stirrer 4 and beta-D glucose is decomposed by glucose oxidase to produce hydrogen peroxide. At the same time, hydrogen peroxide is decomposed by the action of catalase. The concentration of said hydrogen peroxide is detected by a polarographic electrode 1 and its signal is amplified to differentiate by a differential circuit 9 and its maximum value is measured.

公开(公告)号：JPS56118662A

公开(公告)日：1981-09-17

申请号：JP2116980

申请日：1980-02-22

Applicant: KYOWA HAKKO KOGYO KK ,  TOA ELECTRONICS

Inventor： SAKADO KUNIAKI ,  TANAKA HISAO ,  MOTOHASHI RIYOUICHI

IPC: G01N27/416 ,  G01N33/483 ,  G01N33/487

Abstract: PURPOSE:To measure activity, with rapidity and simplicity, by using a liquid containing microbial, animal and botanical tissues, by using an activity measuring device which has a cell equipped with a cathode with built-in anode and cathode, or an activity measuring device which has an electrode, whose cathode and anode are exposed, and a constant potential electrolyzing device. CONSTITUTION:When measuring activities of microbes, such as bacteria and yeast, etc., of blood corpuscle and lymphatic corpuscle, etc. of animal and botanical tissues, etc., a cell 9 which has an anode 3, an anolyte 7, a temperature detecting element 8, a liquid junction 4 such as a ceramic membrane, etc. for making it contact outside liquid, and a cathode 9, is inserted into a tank 10 which is filled with liquid containing these animal and botanical tissues (incubatory liquid and suspension, etc.), and the measurement results are shown by using an ammeter or a voltmeter 2 and a recording device 1. A separate measuring device which has an electrode, equipped with exposed cathode and anode, and a constant potential electrolyzing device can also be used. It is thus possible to measure, with rapidity and simplicity, biological activities of microbial, animal and botanical tissues without requiring a reference battery or a reference electrode.