公开(公告)号：JPS6024450A

公开(公告)日：1985-02-07

申请号：JP13089383

申请日：1983-07-20

Applicant: TORAY INDUSTRIES

Inventor： UCHIDA TAKASHI ,  HOSAKA SHIYUNTAROU

IPC: G01N33/53 ,  A61K39/44 ,  G01N33/532 ,  G01N33/543 ,  G01N33/58

Abstract: PURPOSE:To perform high sensitivity measurement, by performing immunological reaction in the presence of a conjugate of the same substance as a substance to be measured or a substance specifically bonded thereto and biotin, and allowing avidin having chemiluminescent agent or a bioluminescent agent bonded thereto to react with the formed composite. CONSTITUTION:In immunological measurement of a biologically active substance, for example, an antigen, an antibody or medicine and a substance specifically bonded thereto, for example an antibody, a substance, which is obtained by bonding either one of the same substance as a substance to be measured or a substance specifically bonded thereto to biotin, is used as a reagent. For example, in measurement of an antigen in a specimen to be examined, biotin is bonded to the antibody to said antigen to perform reaction or a definite amount of a substance obtained by bonding biotin to the same antigen and the antibody are added. Avidin having a chemiluminescent agent or a bioluminescent agent bonded thereto is reacted with the composite being the generated antigen-antibody biotin conjugate or antibody-antigen-antigen conjugate and the luminous intensity of the luminescent agent bonded to the composite or the unreacted luminescent agent is measured to perform high sensitivity quantitative analysis.

公开(公告)号：JPS577557A

公开(公告)日：1982-01-14

申请号：JP8090780

申请日：1980-06-17

Applicant: TORAY INDUSTRIES

Inventor： HOSAKA SHIYUNTAROU ,  UCHIDA TAKASHI ,  MURAO YASUO

IPC: G01N33/564 ,  G01N33/53 ,  G01N33/543 ,  G01N33/54

Abstract: PURPOSE:To obtain fine particles for agglutination, used for the measurement of the immune composite bodies in a body fluid, which are sensitive and excellent both in maintaining a constant quality and in the preservation stability, by fixing conkurchinine to individually separated fine particles whose particle sizes are within a specified range by means of a physical absorption or chemical bonding. CONSTITUTION:Conkurchinine is physically absorved or chemically bonded to a hydrophobic macromolecular compound, the erythrocyte of an animal which has been treated with formalin and moreover treated with tanic acid or the like, and fine particle carriers of a hydrophilic insoluble substance or the like which has a functional group capable of chemically bonding conkurchinine by means of covalent or ionic bonding. As said fine particles, fine particles of average particle size 0.01- 20mu and individually separated are used. Specially, the fine particles covalent-bonded to a hydrophilic macromolecular compound hardly cause a nonspecific agglutination. The dispersion of sid fine particles and a body fluid are reacted with each other to measure the settling image resulting from an agglutination. Thereby, the measurement of the immune composite bodies in the body fluid is readily performed with high sensitivity.

公开(公告)号：JPS56156213A

公开(公告)日：1981-12-02

申请号：JP5867780

申请日：1980-05-06

Applicant: TORAY INDUSTRIES

Inventor： HOSAKA SHIYUNTAROU ,  MURAO YASUO

IPC: A61K9/14 ,  A61K9/58 ,  C08F8/00 ,  C08F8/12 ,  G01N33/545

Abstract: PURPOSE:To obtain the titled fine particles for clinical tests having a good measuring sensitivity, by polymerizing acrylonitrile or methacrylonitrile into the form of fine particles, hydrolyzing the fine particles, and reacting an immunological active substance with the resultant specific carrier particles. CONSTITUTION:Acrylonitrile or methacrylonitrile is polymerized in a medium, e.g. ethyl propionate, which will dissolve the monomer mixture but precipitate and deposit the resultant polymer in the presence of a crosslinkable monomer, e.g. divinylbenzene, having at least two polymerizable carbon-carbon double bonds to precipitate and deposit a fine particulate polymer having an average diameter of 0.03-10mum. The resultant fine particulate polymer is then hydrolyzed and reacted with an immunologically active substance, e.g. syphilitic treponemal antigen or HBs antigen, to give immunologically active fine particles. The resultant carrier consists of polyacrylamide particles having a uniform particle size, and has very little nonspecific adsorption, e.g. protein, and facilitates the measurement.

公开(公告)号：JPS5883964A

公开(公告)日：1983-05-19

申请号：JP18236481

申请日：1981-11-16

Applicant: TORAY INDUSTRIES

Inventor： UCHIDA TAKASHI ,  HOSAKA SHIYUNTAROU

IPC: D01F6/00 ,  A61M1/36 ,  C12N5/07 ,  C12N5/0781

公开(公告)号：JPS5873866A

公开(公告)日：1983-05-04

申请号：JP17146781

申请日：1981-10-28

Applicant: TORAY INDUSTRIES

Inventor： MURAO YASUO ,  HOSAKA SHIYUNTAROU

IPC: G01N33/543 ,  G01N33/545 ,  G01N33/547

Abstract: PURPOSE:To enable quick and highly sensitive detection and also preliminary preparation and preservation of a capillary tube with reagent therein by a method wherein a reagent for immune agglutination reaction is injected into the capillary tube, frozen and dried and are mixed with a solution to be analyzed, and then the capillary tube is laid horizontally in a stationary state to observe the state of agglutination. CONSTITUTION:A reagent (an antibody for an antigen, an antigen for an antibody, etc.) agglutinating in peculiar reaction to a substance to be measured (an antigen, an antibody, a complement component, etc.) is injected, as it is, into a capillary tube (a glass or plastic tube) having the inside diameter of 0.5-5mm. and the length of 50-100mm., or a reagent for immune agglutination reaction fixed on a carrier such as a glass or plastic grain or a red corpuscle is injected therein. Then it is frozen and dried in liquid nitrogen. The capillary tube, sealed up at both ends, can be preserved in the frozen state until an analysis is conducted. At the time of the analysis, the reagent dissolved or dispersed in water and then mixed with a solution to be analyzed or the four-times dilution thereof. Thereafter, the capillary tube is laid horizontally in a stationary state, and the state of agglutination thus generated is observed. When the agglutination (a) is present, the substance is positive, and when there is a state (b) that the reagent adheres to the wall of the tube, the substance is negative. Thus, the detection can be performed quickly and simply.

公开(公告)号：JPS5796260A

公开(公告)日：1982-06-15

申请号：JP17256380

申请日：1980-12-09

Applicant: TORAY INDUSTRIES

Inventor： HOSAKA SHIYUNTAROU ,  MURAO YASUO

IPC: C08F8/00 ,  C08F8/32 ,  C08J3/20 ,  G01N33/543 ,  G01N33/545

Abstract: PURPOSE:To obtain an inspection reagent by fixing an immunoactive substance with an amino group to a copolymer, having a diameter of 0.03-10mum, of glycidyl acrylate or glycidyl methacrylat and a water-soluble monomer with a carbon unsaturated double bond. CONSTITUTION:It is possible to vary within a range between 95:5 and 5:95 in mol ratio the ratio of the sum of glycidyl acrylate and glycidyl methacrylate in any ratio and the sum of water-soluble monomers such as 2-oxyethyl acrylate, 2-oxyethyl methacrylate or the like. An immunoactive substance is fixed on a carrier particle by means of the covalent bond produced through the reaction between the amino group of the substance and the free epoxy group on the particle surface. The immunoactive substance-fixed particle thus produced is stable to a specimen body fluid, so that a nonspecific agglutination hardly takes place and there is no nonspecific adhesion to cells.

公开(公告)号：JPS5785320A

公开(公告)日：1982-05-28

申请号：JP16137280

申请日：1980-11-18

Applicant: TORAY INDUSTRIES

Inventor： HOSAKA SHIYUNTAROU ,  MURAO YASUO

IPC: A61K39/385 ,  A61K39/00 ,  A61K39/395 ,  A61K39/44 ,  A61M1/36 ,  B01J20/26 ,  G01N33/545

Abstract: PURPOSE:To prepare the titled immuno-active substance, easily and economically by bonding an immuno-active substance to the surface of a solid carrier consisting of the partial hydrolyzate of acrylonitrile fiber. CONSTITUTION:Partially hydrolyzed acrylonitrile fiber (modacryl fiber containing

公开(公告)号：JPS6036962A

公开(公告)日：1985-02-26

申请号：JP14522983

申请日：1983-08-09

Applicant: TORAY INDUSTRIES

Inventor： HOSAKA SHIYUNTAROU ,  UCHIDA TAKASHI

IPC: G01N21/76 ,  C12Q1/02 ,  C12Q1/04 ,  G01N33/50 ,  G01N33/532 ,  G01N33/533 ,  G01N33/534

Abstract: PURPOSE:To enable execution of a biological inspection which is not possible with colored particles, fluorescence-labeled fine particles and metal-labeled fine particles by incorporating a chemiluminescent material into the fine particles. CONSTITUTION:Various natural and artificial fine particles originating in living things or non-living things are usable as fine particles and the fine particles having the diameter in a 0.03-20mum range are used. The shape thereof may be spherical or aspherical but in the case of the aspherical shape, the diameter can be assumed as 1/2 of the sum of the max. diameter and the min. diameter for convenience sake. The particularly preferable material of the fine particles is a synthetic high polymer compd. The chemiluminescent material is enumerated by, for example, luminol, isoluminol, N-(4-aminobutyl)-N-ethylisoluminol, N-(6- aminohexyl)-N-ethylisoluminol, etc. Either of a method for binding chemically such chemiluminescent material or a method for occuluding physically the material is usable as a method for incorporating said material into the fine particles. There are a method using a covalent bond and a method using an ion bond in the method for binding chemically said material.

公开(公告)号：JPS58103465A

公开(公告)日：1983-06-20

申请号：JP20090981

申请日：1981-12-15

Applicant: TORAY INDUSTRIES

Inventor： HOSAKA SHIYUNTAROU ,  MURAO YASUO ,  UCHIDA TAKASHI

IPC: A61M1/36

公开(公告)号：JPS5814057A

公开(公告)日：1983-01-26

申请号：JP11089681

申请日：1981-07-17

Applicant: TORAY INDUSTRIES

Inventor： UCHIDA TAKASHI ,  HOSAKA SHIYUNTAROU

IPC: G01N33/543 ,  G01N33/58

Abstract: PURPOSE:To determine antigen or the like in a sample by measuring the intensity of scattered light of active fine particles by employing active fine particles with a fine particle connected thereto as labeling agent for a matter (antibodies or the like) peculiarly combined with a biologically active matter (antigen, hormon, or the like). CONSTITUTION:A dispersion liquid of active fine particles is prepared for a matter (antibodies agent antigens) peculiarly combined with biologically active matters (antigens, hormons or the like) to be measured by connecting fine particles 0.03mu-3mu in the particle size such as synthetic resin thereto as a labelling agent. On the other hand, a partner (an antibody, an immunoglobulin, or the like) peculiarly combined with the matter being measured is solidified on a hole wall of a microplate and synthetic resin particles (with the particle size of 4mu or more) to prepare a solid phase. After reacting the matter being measured in the sample, the solid phase is separated and reacts a fixed amount of the dispertion liquid the active fine particles. Then, the intensity of scattered light of the active fine particles. Then, the intensity of scattered light of the active fine particles. Then, the intensity of scattered light of the active fine particles left from the reaction with the solid phase is measured. The results are compared to a curve of the intensity of scattered light and the concentration of the matter to be measured previously obtained for a known amount thereof thereby assuring a highly accurate determination thereof.