公开(公告)号：MY166434A

公开(公告)日：2018-06-27

申请号：MYPI2014700057

申请日：2012-01-13

Applicant: TORAY INDUSTRIES

Inventor： YAMADA KATSUSHIGE ,  ISHIKAWA KAZUHIKO ,  MISHIMA YUMIKO ,  WADA YASUNOBU ,  KADO YUJI ,  KURIHARA HIROYUKI

IPC: C12P19/14 ,  C12N9/42 ,  C12P19/02

Abstract: Provided is a novel β-glucosidase exhibiting high activity in the presence of biomass and having high thermal stability compared to conventional enzymes. The β-glucosidase includes substitutions and/or deletions of amino acids, i.e., at least three amino acids selected from the group consisting of Glu39, Asp169, Arg170, Arg220, Tyr227, and Glu330, of the parent β-glucosidase with other amino acids and shows a cellobiose decomposition activity.

公开(公告)号：ES2544710T3

公开(公告)日：2015-09-03

申请号：ES11750425

申请日：2011-01-26

Applicant: TORAY INDUSTRIES

Inventor： KURIHARA HIROYUKI ,  WATANABE SHIOMI ,  YAMADA KATSUSHIGE ,  ISHIKAWA KAZUHIKO ,  WADA YASUNOBU ,  KADO YUJI

IPC: C12N9/42 ,  C12N1/19 ,  C12N1/20 ,  C12N15/09 ,  C12P19/02

Abstract: Procedimiento para producir una β-glucosidasa mutante derivada de un termófilo que tiene fijada selectivamente a ella una cadena de azúcares y también tiene una actividad glucosidasa, que comprende: (i) preparar ADN que codifica una β-glucosidasa mutante derivada de un termófilo introduciendo una secuencia de ADN que codifica Asn-X-Ser o Asn-X-Thr (en las que, X es cualquier aminoácido excepto prolina) en ADN que codifica una β-glucosidasa derivada de un termófilo que está originalmente desprovista de una secuencia de glucosilación y, además, añadiendo una secuencia de ADN que codifica una secuencia señal de secreción al ADN que codifica una β-glucosidasa mutante, (ii) introducir el ADN que codifica una β-glucosidasa mutante al que se le ha añadido la secuencia de ADN que codifica la secuencia señal de secreción en un microorganismo eucariota, de modo que una β-glucosidasa mutante codificada por el ADN de la β-glucosidasa mutante se exprese como una proteína secretora, y (iii) aislar y purificar la β-glucosidasa mutante expresada de este modo como una proteína secretora.

公开(公告)号：AU2012281960A1

公开(公告)日：2014-02-27

申请号：AU2012281960

申请日：2012-01-13

Applicant: TORAY INDUSTRIES

Inventor： KURIHARA HIROYUKI ,  YAMADA KATSUSHIGE ,  ISHIKAWA KAZUHIKO ,  MISHIMA YUMIKO ,  WADA YASUNOBU ,  KADO YUJI

IPC: C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/42 ,  C12P19/14

Abstract: Provided is a novel &bgr;-glucosidase which can exhibit a high activity in the presence of a biomass and has high thermal stability to conventional enzymes. A &bgr;-glucosidase characterized by being produced by substituting and/or deleting at least three amino acid residues selected from the group consisting of Glu39, Asp169, Arg170, Arg220, Tyr227 and Glu330 in the amino acid sequence for a parent &bgr;-glucosidase by another amino acid residue, and exhibiting a cellobiose decomposition activity.

公开(公告)号：DK2735612T3

公开(公告)日：2017-08-21

申请号：DK12811790

申请日：2012-01-13

Applicant: TORAY INDUSTRIES

Inventor： KURIHARA HIROYUKI ,  YAMADA KATSUSHIGE ,  ISHIKAWA KAZUHIKO ,  MISHIMA YUMIKO ,  WADA YASUNOBU ,  KADO YUJI

IPC: C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/42 ,  C12P19/14

公开(公告)号：BR112012021230A2

公开(公告)日：2015-10-27

申请号：BR112012021230

申请日：2011-01-26

Applicant: TORAY INDUSTRIES

Inventor： KURIHARA HIROYUKI ,  YAMADA KATSUSHIGE ,  ISHIKAWA KAZUHIKO ,  WATANABE SHIOMI ,  WADA YASUNOBU ,  KADO YUJI

IPC: C12N1/19 ,  C12N1/20 ,  C12N9/42 ,  C12N15/09 ,  C12P19/02

公开(公告)号：CA2841600A1

公开(公告)日：2013-01-17

申请号：CA2841600

申请日：2012-01-13

Applicant: TORAY INDUSTRIES

Inventor： KURIHARA HIROYUKI ,  YAMADA KATSUSHIGE ,  ISHIKAWA KAZUHIKO ,  MISHIMA YUMIKO ,  WADA YASUNOBU ,  KADO YUJI

IPC: C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/42 ,  C12P19/14

Abstract: Provided is a novel ß-glucosidase which can exhibit a high activity in the presence of a biomass and has high thermal stability to conventional enzymes. A ß-glucosidase characterized by being produced by substituting and/or deleting at least three amino acid residues selected from the group consisting of Glu39, Asp169, Arg170, Arg220, Tyr227 and Glu330 in the amino acid sequence for a parent ß-glucosidase by another amino acid residue, and exhibiting a cellobiose decomposition activity.

公开(公告)号：AU2011222255A1

公开(公告)日：2012-10-11

申请号：AU2011222255

申请日：2011-01-26

Applicant: TORAY INDUSTRIES

Inventor： KURIHARA HIROYUKI ,  WATANABE SHIOMI ,  YAMADA KATSUSHIGE ,  ISHIKAWA KAZUHIKO ,  WADA YASUNOBU ,  KADO YUJI

IPC: C12N9/42 ,  C12N1/19 ,  C12N1/20 ,  C12N15/09 ,  C12P19/02

Abstract: Provided is an efficient method for decomposing biomass, which involves producing glucosidase in which the cellulose degrading efficiency is improved, and using said glucosidase. Specifically, provided is a method for producing a mutant glucosidase wherein a DNA encoding a secretion signal sequence and a DNA encoding Asn-X-Ser or Asn-X-Thr are introduced into a DNA encoding a glucosidase from a thermophile, and the resulting product is introduced into a eukaryotic microorganism and expressed as a secretory protein. Also provided are an enzyme composition containing said mutant glucosidase, and a biomass hydrolysis method using said enzyme composition.

公开(公告)号：AU2012281960B2

公开(公告)日：2017-02-02

申请号：AU2012281960

申请日：2012-01-13

Applicant: TORAY INDUSTRIES

Inventor： KURIHARA HIROYUKI ,  YAMADA KATSUSHIGE ,  ISHIKAWA KAZUHIKO ,  MISHIMA YUMIKO ,  WADA YASUNOBU ,  KADO YUJI

IPC: C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/42 ,  C12P19/14

Abstract: Provided is a novel β-glucosidase which can exhibit a high activity in the presence of a biomass and has high thermal stability to conventional enzymes. A β-glucosidase characterized by being produced by substituting and/or deleting at least three amino acid residues selected from the group consisting of Glu39, Asp169, Arg170, Arg220, Tyr227 and Glu330 in the amino acid sequence for a parent β-glucosidase by another amino acid residue, and exhibiting a cellobiose decomposition activity.

公开(公告)号：DK2543723T3

公开(公告)日：2015-09-21

申请号：DK11750425

申请日：2011-01-26

Applicant: TORAY INDUSTRIES

Inventor： KURIHARA HIROYUKI ,  WATANABE SHIOMI ,  YAMADA KATSUSHIGE ,  ISHIKAWA KAZUHIKO ,  WADA YASUNOBU ,  KADO YUJI

IPC: C12N9/42 ,  C12N1/19 ,  C12N1/20 ,  C12N15/09 ,  C12P19/02

公开(公告)号：AU2011222255B2

公开(公告)日：2014-11-06

申请号：AU2011222255

申请日：2011-01-26

Applicant: TORAY INDUSTRIES

Inventor： KURIHARA HIROYUKI ,  WATANABE SHIOMI ,  YAMADA KATSUSHIGE ,  ISHIKAWA KAZUHIKO ,  WADA YASUNOBU ,  KADO YUJI

IPC: C12N9/42 ,  C12N1/19 ,  C12N1/20 ,  C12N15/09 ,  C12P19/02

Abstract: Provided is an efficient method for decomposing biomass, which involves producing glucosidase in which the cellulose degrading efficiency is improved, and using said glucosidase. Specifically, provided is a method for producing a mutant glucosidase wherein a DNA encoding a secretion signal sequence and a DNA encoding Asn-X-Ser or Asn-X-Thr are introduced into a DNA encoding a glucosidase from a thermophile, and the resulting product is introduced into a eukaryotic microorganism and expressed as a secretory protein. Also provided are an enzyme composition containing said mutant glucosidase, and a biomass hydrolysis method using said enzyme composition.