-
公开(公告)号：DE68927437T2
公开(公告)日：1997-03-06
申请号：DE68927437
申请日：1989-08-09
Applicant: TORAY INDUSTRIES
Inventor： KAZAMI JUN , NAKAMURA HARUJI , GOTO TOSHIO
Abstract: A luciferase having a 555 amino-acid sequence (specified in the patent) and its derivs. (such as fragments of the luciferase consisting of residues 29-555; 30-55; 31-555 or 32-555) are new. Also claimed are a DNA sequence coding for the luciferase or its derivs.; a DNA vector contg. the DNA sequence connected to the downstream side of a suitable promoter and SD sequence (e.g. derived from a coliform bacterium); and a transformant organism contg. the vector (e.g. an animal cell, yeast or coliform bacterium) and capable of expressing the DNA sequence and forming the luciferase.
-
公开(公告)号：DE68927437D1
公开(公告)日：1996-12-12
申请号：DE68927437
申请日：1989-08-09
Applicant: TORAY INDUSTRIES
Inventor： KAZAMI JUN , NAKAMURA HARUJI , GOTO TOSHIO
Abstract: A luciferase having a 555 amino-acid sequence (specified in the patent) and its derivs. (such as fragments of the luciferase consisting of residues 29-555; 30-55; 31-555 or 32-555) are new. Also claimed are a DNA sequence coding for the luciferase or its derivs.; a DNA vector contg. the DNA sequence connected to the downstream side of a suitable promoter and SD sequence (e.g. derived from a coliform bacterium); and a transformant organism contg. the vector (e.g. an animal cell, yeast or coliform bacterium) and capable of expressing the DNA sequence and forming the luciferase.
-
公开(公告)号：AT145004T
公开(公告)日：1996-11-15
申请号：AT89909235
申请日：1989-08-09
Applicant: TORAY INDUSTRIES
Inventor： KAZAMI JUN , NAKAMURA HARUJI , GOTO TOSHIO
Abstract: A luciferase having a 555 amino-acid sequence (specified in the patent) and its derivs. (such as fragments of the luciferase consisting of residues 29-555; 30-55; 31-555 or 32-555) are new. Also claimed are a DNA sequence coding for the luciferase or its derivs.; a DNA vector contg. the DNA sequence connected to the downstream side of a suitable promoter and SD sequence (e.g. derived from a coliform bacterium); and a transformant organism contg. the vector (e.g. an animal cell, yeast or coliform bacterium) and capable of expressing the DNA sequence and forming the luciferase.
-
Patent Agency Ranking
公开(公告)号：JPH0827200A
公开(公告)日：1996-01-30
申请号：JP16522394
申请日：1994-07-18
Applicant: TORAY INDUSTRIES
Inventor： SUZUKI EIJI , UEDA HIROSHI , KAZAMI JUN , KONO HAJIME
IPC: G01N33/53 , C07K14/195 , C07K14/41 , C07K14/435 , C07K16/00 , C07K19/00 , C12N9/02 , C12N15/09 , C12P21/02 , C12Q1/66 , C12Q1/68 , C12R1/91 , G01N33/535
Abstract: PURPOSE:To obtain a fused protein capable of being simply mixed with IgG to label the IgG and useful as a labeled enzyme used for luminescent immunoassay methods, etc., by binding a peptide containing an IgG-recognizing region to the end of Cypridina luciferase by a genetic engineering method. CONSTITUTION:The new fused protein capable of being simply mixed with IgG to label the IgG with Cypridina luciferase without requiring a chemical treatment for the labeling and useful as a labeling enzyme for luminescent immunoassay methods is obtained by binding a peptide containing a region recognizing the IgG Fc part of a protein A originated from Staphylococcus aureus, a peptide containing a region recognizing the IgG Fc part of a protein C originated from a streptococcus, etc., to the amino end of the Cypridina luciferase. The fused protein is obtained by expressing a vector which is produced by binding the gene of a peptide having a region recognizing the IgG to the 5'-site of Cypridina luciferase gene through a linker.
-
公开(公告)号：JP2002223770A
公开(公告)日：2002-08-13
申请号：JP2001025488
申请日：2001-02-01
Applicant: TORAY INDUSTRIES
Inventor： MIMIZUKA TAKASHI , KAZAMI JUN
Abstract: PROBLEM TO BE SOLVED: To more profitably produce cadaverine. SOLUTION: This method for producing the cadaverine comprises inserting a lysine decarbonation enzyme gene and/or a lysine-cadaverine antiporter gene into a microorganism and allowing to highly express the cadaverine, thereby accumulating the cadaverine in a high concentration in the microorganism in which the activity of the lysine decarbonation enzyme gene and/or the lysine- cadaverine antiporter gene are enhanced.
-
公开(公告)号：JPH0956384A
公开(公告)日：1997-03-04
申请号：JP21691195
申请日：1995-08-25
Applicant: TORAY INDUSTRIES
Inventor： NAGAMUNE TERUYUKI , UEDA HIROSHI , KAZAMI JUN , KONO HAJIME
Abstract: PROBLEM TO BE SOLVED: To obtain a labeled cell capable of being detected only by being mixed with a luminescent substrate without requiring a preliminary treating operation by ligating a gene expressing on a cell membrane to the gene of a secretion type luminescent enzyme, and subsequently expressing the enzyme as a fused protein on the cell membrane. SOLUTION: A method for labeling a cell comprises cutting out the gene of the cell membrane-penetrating region of an epithelial cell-multiplying factor receptor as a gene expressing on a cell membrane from a plasmid containing the gene with a restriction enzyme, ligating the obtained gene to the gene of a secretion type luminescent enzyme such as the luciferase of Cypridina vargula, recombining the ligation product into an expression vector, introducing the prepared recombinant DNA into an animal cell such as a COS-1 cell or a CHO cell, and expressing the gene as a fused protein on the cell membrane. Thus, the labeled cell capable of being detected and fractionated with an optical signal by a flow cytometry method, etc., is obtained only by mixing the labeled cell with a luminescent substrate.
-
公开(公告)号：JP2002223771A
公开(公告)日：2002-08-13
申请号：JP2001025489
申请日：2001-02-01
Applicant: TORAY INDUSTRIES
Inventor： MIMIZUKA TAKASHI , KAZAMI JUN
Abstract: PROBLEM TO BE SOLVED: To more profitably produce cadaverine. SOLUTION: This method for producing the cadaverine comprises allowing a crudely purified lysine decarbonation enzyme to act on lysine to produce cadaverine, and then collecting the produced cadaverine. The crudely purified lysine decarbonation enzyme is obtained by inserting a lysine decarbonation enzyme gene into Escherichia coli, allowing to highly express the lysine decarbonation enzyme, and then separating the lysine decarbonation enzyme from the recombinant microorganism.
-
公开(公告)号：JP2002136293A
公开(公告)日：2002-05-14
申请号：JP2001252395
申请日：2001-08-23
Applicant: TORAY INDUSTRIES
Inventor： SASAKI SATOSHI , KAZAMI JUN
Abstract: PROBLEM TO BE SOLVED: To more profitably produce D-lactic acid. SOLUTION: The problem of the present invention can be solved by inserting a D-lactic acid dehydrogenase gene into a highly pyruvic acid-producing yeast and then highly expressing D-lactic acid to accumulate the D-lactic acid in a high concentration. The D-lactic acid is obtained by culturing a microorganism obtained by transducing a D-lactic acid dehydrogenase-encoding gene into a microorganism having an ability to accumulate pyruvic acid in a concentration of >=10 g/L. A yeast such as Torulopsis glabrata or Torulopsis methanalvescence is preferably used as the microoganism.
-
-
-
-
-
-
-
Search Results
8
records
(took 0.021 seconds)
