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    THREAD SPOOLING METHOD
    6.
    发明专利
    THREAD SPOOLING METHOD 失效

    公开(公告)号：JPS5785767A

    公开(公告)日：1982-05-28

    申请号：JP16045280

    申请日：1980-11-14

    Applicant: TORAY INDUSTRIES

    Inventor： IMAE MASAZUMI ,  UEDA HIROSHI

    IPC: B65H59/38 ,  B65H67/04

    Abstract: PURPOSE:To stabilize abnormal tension likely to occur at the time of hooking a thread on a bobbin in a short period of time by incorporating a spindle number- of-revolutions setting device for driving the spindle at a higher rotational speed than the initially set number of revolutions. CONSTITUTION:A tension pickup 4 is furnished on the supply side of a winder 1 and a signal according to this tension is placed into a comparator 3, which is to compare this signal with the spindle number-of-revolutions signal set by a presetting device 2, in order to control rotation of the spindle of the winder 1 so that the difference in outputs becomes zero and thus to maintain the takeup tension at a certain specific value. The spindle number-of-revolutions setting device 5 is equipped with a switch 5a which is turned off by a thread hooking completion signal and, when the switch 5a is one, a number of revolutions higher by D than the initially set number of revolutions for the spindle in the spooling control system is given.

    LABELING OF CELL
    8.
    发明专利
    LABELING OF CELL 失效

    公开(公告)号：JPH0956384A

    公开(公告)日：1997-03-04

    申请号：JP21691195

    申请日：1995-08-25

    Applicant: TORAY INDUSTRIES

    Inventor： NAGAMUNE TERUYUKI ,  UEDA HIROSHI ,  KAZAMI JUN ,  KONO HAJIME

    IPC: G01N33/48 ,  C07H21/04 ,  C12N5/10 ,  C12N9/02 ,  C12N15/09 ,  C12P21/02 ,  C12Q1/66 ,  G01N21/76

    Abstract: PROBLEM TO BE SOLVED: To obtain a labeled cell capable of being detected only by being mixed with a luminescent substrate without requiring a preliminary treating operation by ligating a gene expressing on a cell membrane to the gene of a secretion type luminescent enzyme, and subsequently expressing the enzyme as a fused protein on the cell membrane. SOLUTION: A method for labeling a cell comprises cutting out the gene of the cell membrane-penetrating region of an epithelial cell-multiplying factor receptor as a gene expressing on a cell membrane from a plasmid containing the gene with a restriction enzyme, ligating the obtained gene to the gene of a secretion type luminescent enzyme such as the luciferase of Cypridina vargula, recombining the ligation product into an expression vector, introducing the prepared recombinant DNA into an animal cell such as a COS-1 cell or a CHO cell, and expressing the gene as a fused protein on the cell membrane. Thus, the labeled cell capable of being detected and fractionated with an optical signal by a flow cytometry method, etc., is obtained only by mixing the labeled cell with a luminescent substrate.

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