公开(公告)号：JPS6062991A

公开(公告)日：1985-04-11

申请号：JP16904283

申请日：1983-09-13

Applicant: TORAY INDUSTRIES

Inventor： IMAMURA SHINZOU ,  TANAKA NORIKO ,  MIYATA REIKO

IPC: C12P13/04 ,  C12P41/00 ,  C12R1/01 ,  C12R1/37 ,  C12R1/72

Abstract: PURPOSE:To collect a converted L-amino acid in high yield, by treating an L- acyl-DL-amino acid with acylase produced by a fungus belonging to the genus Cryptococcus. CONSTITUTION:A fungus capable of producing acylase, belonging to the genus Cryptococcus, Candida, Trichosporon, is cultivated in a liquid medium. This cultivation is usually carried out by aerated spinner culture at 30-35 deg.C at 6- 8pH. The culture is directly used as an enzyme source, or a culture such as a cell-free extracted solution obtained by grinding the separated mold or mold, or separated acylase is used as the enzyme source. Namely, this acylase is made to act on an N-acyl-DL-amino acid at 30-35 deg.C at 6.0-7.0pH, it is asymmetrically hydrolyzed, to give an L-amino acid (e.g., L-methionine). In the asymmetric hydrolysis, the presence of a metal ion such as Co ion in the reaction solution promotes the reaction, and the reaction can be completed in 3-20hr.

公开(公告)号：JPH0677B2

公开(公告)日：1994-01-05

申请号：JP21245584

申请日：1984-10-12

Applicant: TORAY INDUSTRIES

Inventor： SODA KENJI ,  SATO HARUYO ,  TANAKA NORIKO ,  IMAMURA SHINZO

IPC: C07C59/08 ,  C07C51/00 ,  C07C51/02 ,  C07C51/367 ,  C07C67/00 ,  C12P7/56 ,  C12P41/00 ,  C12R1/40

公开(公告)号：JPH0577662B2

公开(公告)日：1993-10-27

申请号：JP18283184

申请日：1984-09-03

Applicant: TORAY INDUSTRIES

Inventor： SATO HARUYO ,  IMAMURA SHINZO ,  TANAKA NORIKO

IPC: C07B61/00 ,  B01J31/00 ,  B01J31/08 ,  C07C67/08 ,  C07C69/68