公开(公告)号：ES2916837T3

公开(公告)日：2022-07-06

申请号：ES16773204

申请日：2016-03-31

Applicant: TORAY INDUSTRIES

Inventor： OTSUKA HIROKI ,  UEDA YOJI

IPC: G01N35/02 ,  B01L3/00 ,  C12Q1/6825 ,  C12Q1/6837 ,  G01N37/00

Abstract: Un chip de análisis de acuerdo con la presente invención incluye un cuerpo principal del sustrato que tiene una pluralidad de porciones de reacción en la que se inmoviliza una sustancia de unión selectiva que se une selectivamente a una sustancia a examinar; una porción de esquina en la que diferentes líneas rectas o líneas curvas se cruzan entre sí en una sección transversal en la que un plano que pasa a través de una superficie del cuerpo principal del sustrato en el que se proporcionan las porciones de reacción es una superficie de corte; Una porción de partición formada aplicando tratamiento repelente al agua a la superficie del cuerpo principal del sustrato en el que se proporcionan las porciones de reacción y dividiendo las porciones de reacción dentro de un borde exterior formado por la superficie, y una porción de conexión que tiene repelencia de agua y se conecta entre una parte de una parte de la porción de partición y la parte de la esquina. (Traducción automática con Google Translate, sin valor legal)

公开(公告)号：CA2877236A1

公开(公告)日：2013-12-27

申请号：CA2877236

申请日：2013-06-19

Applicant: TORAY INDUSTRIES

Inventor： NAKAMURA FUMIO ,  UEDA YOJI ,  ARIKE TAKAFUMI

IPC: C12Q1/68 ,  C12N15/09

Abstract: Provided is a method for detecting a nucleic acid with which it is possible to inhibit separation of the target nucleic acid that has been hybridized with a capture probe from a substrate during the process of labeling by post-staining and to detect the target nucleic acid at a concentration that is lower than with the sodium ion method but at the same or higher sensitivity. The method for detecting the target nucleic acid comprises (1) a step for forming a double stranded nucleic acid by hybridizing the capture probe and target nucleic acid, (2) a step for bringing the resulting double stranded nucleic acid into contact with a solution containing a label and a divalent metal cation at a concentration of 10 mM or higher in order to introduce the label to the double stranded nucleic acid, and (3) a step for detecting the label that has been introduced into the double stranded nucleic acid.

公开(公告)号：CA2865541A1

公开(公告)日：2013-09-06

申请号：CA2865541

申请日：2013-02-27

Applicant: TORAY INDUSTRIES

Inventor： UEDA YOJI ,  NAKAMURA FUMIO

IPC: C12N15/09 ,  C12Q1/68

Abstract: Disclosed is a nucleic acid detection method capable of detecting target nucleic acids with a high degree of sensitivity, even when detecting the target nucleic acids by a sandwich hybridization assay without using a nucleic acid amplification technique or sensitization technique. The method for detecting a target nucleic acid includes: a step in which a plurality of different detection probes, capture probes immobilized on a support, and target nucleic acids or fragments thereof are sequentially or simultaneously brought into contact with each other so as to induce the hybridization of the capture probes and the target nucleic acids or the fragments thereof and the hybridization of the detection probes and the target nucleic acids or the fragments thereof and thereby bond the detection probes to the support via the capture probes and the target nucleic acids or fragments thereof; and a step in which the detection probes bonded to the support are detected.

公开(公告)号：CA2877236C

公开(公告)日：2021-03-09

申请号：CA2877236

申请日：2013-06-19

Applicant: TORAY INDUSTRIES

Inventor： NAKAMURA FUMIO ,  UEDA YOJI ,  ARIKE TAKAFUMI

IPC: C12Q1/6816 ,  C12Q1/6813

Abstract: Provided is a method for detecting a nucleic acid with which it is possible to inhibit separation of the target nucleic acid that has been hybridized with a capture probe from a substrate during the process of labeling by post-staining and to detect the target nucleic acid at a concentration that is lower than with the sodium ion method but at the same or higher sensitivity. The method for detecting the target nucleic acid comprises (1) a step for forming a double stranded nucleic acid by hybridizing the capture probe and target nucleic acid, (2) a step for bringing the resulting double stranded nucleic acid into contact with a solution containing a label and a divalent metal cation at a concentration of 10 mM or higher in order to introduce the label to the double stranded nucleic acid, and (3) a step for detecting the label that has been introduced into the double stranded nucleic acid.

公开(公告)号：CA2981131A1

公开(公告)日：2016-10-06

申请号：CA2981131

申请日：2016-03-31

Applicant: TORAY INDUSTRIES

Inventor： OTSUKA HIROKI ,  UEDA YOJI

IPC: G01N35/02 ,  G01N37/00

Abstract: An analysis chip according to the present invention is provided with: a substrate main body having multiple reaction sections onto which a selectively bonding substance capable of bonding to an analyte selectively is immobilized; a corner section in which different lines or curves intersect each other on a cross section that corresponds to a plane passing through a reaction-section-provided surface of the substrate main body; partitioning sections which are formed by subjecting the reaction section-provided surface of the substrate main body to a water-repellent treatment and which can partition multiple reaction sections in the inside of the outer periphery of the aforementioned surface; and a connection section which has water repellency and connects some of the partitioning sections to the corner section.

公开(公告)号：CA2883499C

公开(公告)日：2020-07-14

申请号：CA2883499

申请日：2013-08-29

Applicant: TORAY INDUSTRIES

Inventor： HIRANO TAISUKE ,  NAKAMURA FUMIO ,  UEDA YOJI ,  FUJIMOTO KENZO

IPC: C12Q1/6832 ,  C12Q1/6813

Abstract: A method for detecting a target nucleic acid, which enables highly sensitive detection of the target nucleic acid without lowering the detection sensitivity (S/N ratio) even by use of a reduced amount of a detection probe, is disclosed. The method for detecting a target nucleic acid is based on sandwich hybridization using a detection probe that hybridizes with the target nucleic acid, and a capture probe immobilized on a support. In this method, at least one nucleic acid base in the nucleic acid molecule(s) of the detection probe and/or capture probe is substituted with a photoreactive group. The method comprises the step of irradiating, with light, a complex formed by hybridization between the target nucleic acid and the detection probe and/or capture probe to allow formation of a covalent bond(s) between the photoreactive group(s) and a nucleic acid base(s) in the target nucleic acid.

公开(公告)号：CA2865541C

公开(公告)日：2019-11-05

申请号：CA2865541

申请日：2013-02-27

Applicant: TORAY INDUSTRIES

Inventor： UEDA YOJI ,  NAKAMURA FUMIO

IPC: C12N15/09

Abstract: Disclosed is a method for detecting nucleic acid with high sensitivity even in cases where the target nucleic acid is detected by sandwich hybridization using neither nucleic acid amplification nor a sensitization technique. The method for detecting a target nucleic acid comprises the steps of: sequentially or simultaneously bringing a target nucleic acid or fragmentation product thereof, a plurality of detection probes, and a capture probe immobilized on a support, into contact with each other to hybridize the capture probe with the target nucleic acid or fragmentation product thereof and to hybridize the target nucleic acid or fragmentation product thereof with the plurality of detection probes, thereby binding the plurality of detection probes to the support through the capture probe and the target nucleic acid or fragmentation product thereof; and then detecting the plurality of detection probes bound to the support.

公开(公告)号：CA2883499A1

公开(公告)日：2014-03-06

申请号：CA2883499

申请日：2013-08-29

Applicant: TORAY INDUSTRIES

Inventor： HIRANO TAISUKE ,  NAKAMURA FUMIO ,  UEDA YOJI ,  FUJIMOTO KENZO

IPC: C12Q1/68 ,  C12N15/09

Abstract: Disclosed is a method for detecting a target nucleic acid, which can detect the target nucleic acid with high sensitivity without deteriorating detection sensitivity (an S/N ratio) even when the amount of a detection probe to be used is reduced. A method for detecting a target nucleic acid by a sandwich hybridization method using a detection probe capable of hybridizing with the target nucleic acid and a capture probe immobilized on a support, wherein at least one nucleotide in a nucleic acid molecule of the detection probe and/or the capture probe is substituted by a photoreactive group. The method involves a step of irradiating a complex, which is formed by the hybridization of the target nucleic acid with the detection probe and/or the capture probe, with light to thereby form a covalent bond between the photoreactive group and the nucleotide in the target nucleic acid.

公开(公告)号：EP2891721A4

公开(公告)日：2016-04-06

申请号：EP13832897

申请日：2013-08-29

Applicant: TORAY INDUSTRIES

Inventor： HIRANO TAISUKE ,  NAKAMURA FUMIO ,  UEDA YOJI ,  FUJIMOTO KENZO

IPC: C12Q1/68 ,  C12N15/09

CPC classification number: C12Q1/6825 ,  C12Q1/6834 ,  C12Q2523/313 ,  C12Q2537/125 ,  C12Q2565/519

Abstract: A method for detecting a target nucleic acid, which enables highly sensitive detection of the target nucleic acid without lowering the detection sensitivity (S/N ratio) even by use of a reduced amount of a detection probe, is disclosed. The method for detecting a target nucleic acid is based on sandwich hybridization using a detection probe that hybridizes with the target nucleic acid, and a capture probe immobilized on a support. In this method, at least one nucleic acid base in the nucleic acid molecule(s) of the detection probe and/or capture probe is substituted with a photoreactive group. The method comprises the step of irradiating, with light, a complex formed by hybridization between the target nucleic acid and the detection probe and/or capture probe to allow formation of a covalent bond(s) between the photoreactive group(s) and a nucleic acid base(s) in the target nucleic acid.

公开(公告)号：EP2865764A4

公开(公告)日：2016-03-23

申请号：EP13807796

申请日：2013-06-19

Applicant: TORAY INDUSTRIES

Inventor： NAKAMURA FUMIO ,  UEDA YOJI ,  ARIKE TAKAFUMI

IPC: C12Q1/68 ,  C12N15/09

CPC classification number: C12Q1/6806 ,  C12Q1/6825 ,  C12Q1/6834 ,  C12Q2527/137 ,  C12Q2563/131 ,  C12Q2563/137

Abstract: Disclosed is a method for detecting a nucleic acid using a substance that suppresses, in the labeling step of the post-staining method, detachment of a target nucleic acid that has once hybridized with a capture probe immobilized on a support, which method enables detection of the target nucleic acid with a sensitivity equivalent to or higher than that achieved by a method using sodium ion even in cases where the substance is used at a lower concentration. The method for detecting a nucleic acid comprises the steps of: (1) hybridizing a capture probe with a target nucleic acid to form a double-stranded nucleic acid; bringing the formed double-stranded nucleic acid into contact with a solution containing a labeling substance and a divalent metal cation at a concentration of not less than 10 mM to introduce the labeling substance into the double-stranded nucleic acid; and detecting the labeling substance introduced into the double-stranded nucleic acid.