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    5.
    发明专利
    未知

    公开(公告)号：NO20081832L

    公开(公告)日：2008-07-25

    申请号：NO20081832

    申请日：2008-04-16

    Applicant: TOSOH CORP

    Inventor： TANAKA TORU ,  IDE TERUHIKO ,  MURAYAMA KEI-ICHI ,  OE SEIGOU ,  IMAIZUMI TORU ,  HANZAWA SATOSHI ,  TOYOSHIMA TOSHINOBU

    IPC: C12N1/20 ,  C12P23/00 ,  C12R1/01

    Abstract: To provide a microorganism capable of producing a large amount of carotenoids, mainly astaxanthin, and to provide a method of producing carotenoids using the microorganism, a microorganism having improved carotenoid productivity is used, which is obtainable by breeding a carotenoid producing Paracoccus bacteria which is featured by producing 720 mg or more carotenoid per 1 L of culture medium. Also used is a method of producing carotenoids using the bacterium.

    Method for producing protein with gene recombination escherichia coli
    8.
    发明专利
    Method for producing protein with gene recombination escherichia coli 审中-公开
    用基因重组蛋白质生产蛋白质的方法

    公开(公告)号：JP2013188141A

    公开(公告)日：2013-09-26

    申请号：JP2012054544

    申请日：2012-03-12

    Applicant: Tosoh Corp ,  東ソー株式会社

    Inventor： IMAIZUMI NOBORU ,  HANZAWA SATOSHI

    IPC: C12P21/00 ,  C07K14/705 ,  C07K16/28 ,  C12N15/09

    Abstract: PROBLEM TO BE SOLVED: To provide a dissolved oxygen concentration and an oxygen-supplying method optimal for efficiently producing a protein, in a method for producing the protein by culturing Escherichia coli capable of expressing the protein and obtained by transforming Escherichia coli with an expression vector containing a polynucleotide encoding the protein.SOLUTION: A method for producing a protein with gene recombination Escherichia coli comprises culturing recombination Escherichia coli obtained by transforming Escherichia coli with an expression vector containing a polynucleotide encoding the protein in a condition having a dissolved oxygen concentration of 10-60%. Specifically, the culture is carried out in a condition having an oxygen-moving speed of ≥200 mmol/(L-h), a gauge pressure of ≥0.05 MPa, or an oxygen partial pressure of ≥30% in a flowing gas.

    Abstract translation: 要解决的问题：为了提供溶解氧浓度和用于有效生产蛋白质的最佳的供氧方法，在通过培养能够表达蛋白质并通过用表达载体转化大肠杆菌获得的大肠杆菌生产蛋白质的方法中 含有编码该蛋白质的多核苷酸。解决方案：用于产生具有基因重组的蛋白质的方法大肠杆菌包括通过在溶解氧浓度为10的条件下，用含有编码蛋白质的多核苷酸的表达载体培养重组大肠杆菌获得的重组大肠杆菌 -60％。 具体而言，在流动气体中，在氧移动速度≥200mmol/（L-h），表压≥0.05MPa，氧分压≥30％的条件下进行培养。

    Method for producing protein with recombinant e.coli
    9.
    发明专利
    Method for producing protein with recombinant e.coli 审中-公开
    用重组蛋白生产蛋白质的方法

    公开(公告)号：JP2013085531A

    公开(公告)日：2013-05-13

    申请号：JP2011230579

    申请日：2011-10-20

    Applicant: Tosoh Corp ,  東ソー株式会社

    Inventor： IMAIZUMI NOBORU ,  HANZAWA SATOSHI

    IPC: C12P21/02 ,  C12N15/09

    Abstract: PROBLEM TO BE SOLVED: To attain an optimum concentration of a metal salt, especially a magnesium salt for efficiently producing a protein, especially an Fc binding protein in a method for producing the protein by culturing an E.coli capable of expressing the protein, and to provide a method for supplying the metal salt.SOLUTION: A recombinant E.coli obtained by transforming E.coli W3110 strain (ATCC 27235) with an expression vector containing a polynucleotide encoding the protein is cultured in a medium added with at least 2.8×10mol of a magnesium salt in terms of magnesium ion based on 1L of the medium.

    Abstract translation: 要解决的问题：为了获得金属盐特别是镁盐的最佳浓度，用于通过培养能够表达该蛋白质的大肠杆菌来生产蛋白质，特别是Fc结合蛋白，特别是Fc结合蛋白 蛋白质，并提供供给金属盐的方法。 解决方案：将含有编码蛋白质的多核苷酸的表达载体转化大肠杆菌W3110菌株（ATCC 27235）获得的重组大肠杆菌在加入至少2.8×10 SP SP =“POST”的培养基中培养 基于1L培养基，以镁离子计算的镁盐的摩尔数> -2 。 版权所有（C）2013，JPO＆INPIT

    METHOD FOR PURIFYING Fc RECEPTOR
    10.
    发明专利
    METHOD FOR PURIFYING Fc RECEPTOR 有权
    纯化Fc受体的方法

    公开(公告)号：JP2012250945A

    公开(公告)日：2012-12-20

    申请号：JP2011126084

    申请日：2011-06-06

    Applicant: Tosoh Corp ,  東ソー株式会社

    Inventor： TERAO YOSUKE ,  HANZAWA SATOSHI

    IPC: C07K14/735 ,  C12N15/09

    Abstract: PROBLEM TO BE SOLVED: To provide a method for highly purely purifying an Fc receptor at a high recovery rate by using ion exchange chromatography.SOLUTION: A solution containing the Fc receptor is added to a carrier for positive ion exchange chromatography to allow the carrier to adsorb the Fc receptor, and the Fc receptor adsorbed on the carrier is eluted by using eluate, to thereby purify the Fc receptor. In this case, urea is included into a solution containing the Fc receptor to be added to the carrier for the positive ion exchange chromatography, and into the eluate.

    Abstract translation: 待解决的问题：提供通过使用离子交换色谱法以高回收率高度纯化Fc受体的方法。 溶液：将含有Fc受体的溶液加入到用于阳离子交换层析的载体中以允许载体吸附Fc受体，并且通过使用洗脱液洗脱吸附在载体上的Fc受体，从而纯化Fc 受体。 在这种情况下，将尿素包含在含有待加入用于阳性离子交换色谱的载体中的Fc受体的溶液中并进入洗脱液中。 版权所有（C）2013，JPO＆INPIT

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