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24 records (took 0.026 seconds)

    1.
    发明专利
    未知

    公开(公告)号：DE602005014025D1

    公开(公告)日：2009-06-04

    申请号：DE602005014025

    申请日：2005-02-02

    Applicant: TOSOH CORP

    Inventor： MASUDA NORIYOSHI ,  YASUKAWA KIYOSHI ,  HORIE RYUICHI

    IPC: C12Q1/68

    Abstract: The present invention provides a detection method for detecting norovirus, using an RNA amplification process comprising the steps of : producing a cDNA with an RNA-dependent DNA polymerase using a specific sequence of norovirus genome RNA as a template, as well as a specifically defined first primer having a sequence homologous to said specific sequence, and a specifically defined second primer having a sequence complementary to said specific sequence, thereby forming a double-strand RNA-DNA, wherein either the first primer or the second primer has a sequence in which a promoter sequence of an RNA polymerase has been added to its 5' end; degrading the RNA portion of said double-strand RNA-DNA by ribonuclease H, thereby producing a single-strand DNA; and producing a double-strand DNA having said promoter sequence capable of transcribing the RNA composed of the specific sequence of the RNA or the sequence complementary to said specific sequence of the RNA with a DNA-dependent DNA polymerase using said single-strand DNA as a template; wherein, the double-strand DNA produces an RNA transcription product in the presence of the RNA polymerase, and said RNA transcription product serves as a template for the subsequent cDNA synthesis with the RNA-dependent DNA polymerase.

    3.
    发明专利
    未知

    公开(公告)号：DE602004005936T2

    公开(公告)日：2007-09-06

    申请号：DE602004005936

    申请日：2004-08-31

    Applicant: TOSOH CORP

    Inventor： MASUDA NORIYOSHI ,  HORIE RYUICHI ,  YASUKAWA KIYOSHI

    IPC: C12Q1/68 ,  G01N33/53 ,  C12N15/09 ,  G01N21/78 ,  G01N33/566 ,  G01N33/569

    Abstract: A detection reagent for detecting thermostable direct hemolysin-related hemolysin (TRH) gene in an amplification process which specifically amplifies TRH1 and TRH2 RNA, which reagent comprises a first primer having a sequence complementary to a specific sequence of an RNA derived from the TRH gene, and a second primer having a sequence homologous to said specific sequence.

    4.
    发明专利
    未知

    公开(公告)号：DE602004005936D1

    公开(公告)日：2007-05-31

    申请号：DE602004005936

    申请日：2004-08-31

    Applicant: TOSOH CORP

    Inventor： MASUDA NORIYOSHI ,  HORIE RYUICHI ,  YASUKAWA KIYOSHI

    IPC: C12Q1/68 ,  G01N33/53 ,  C12N15/09 ,  G01N21/78 ,  G01N33/566 ,  G01N33/569

    Abstract: A detection reagent for detecting thermostable direct hemolysin-related hemolysin (TRH) gene in an amplification process which specifically amplifies TRH1 and TRH2 RNA, which reagent comprises a first primer having a sequence complementary to a specific sequence of an RNA derived from the TRH gene, and a second primer having a sequence homologous to said specific sequence.

    7.
    发明专利
    未知

    公开(公告)号：AT470710T

    公开(公告)日：2010-06-15

    申请号：AT02749365

    申请日：2002-07-24

    Applicant: TOSOH CORP

    Inventor： TSUCHIYA SHIGEO ,  MASUDA NORIYOSHI ,  MARUYAMA TAKAHIRO ,  MATSUBA TAKAO ,  ISHIGURO TAKAHIKO

    IPC: C12N15/00 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/566 ,  G01N33/569 ,  G01N33/58

    Abstract: Oligonucleotides comprising not less than 10 consecutive bases in the sequences of (I)-(XX) with 20-23 base pairs for use in cleaving, detecting or amplifying essential pab genes of tubercle bacillus or RNA originated from them and capable of binding specifically with such genes or RNA, or their complementary oligonucleotides, are new. An independent claim is also included for a method for the detection of tubercle bacillus-originated pab genes comprising: (a) using specific sequences of the pab genes or their derived RNA in a sample as template, and a first primer containing sequences analogous to such specific sequences and a second primer containing sequences complementary to these specific sequences (provided that any of the first and second primers has a sequence attached with a promoter sequence of RNA polymerase at its 5'-side), with RNA-dependent DNA polymerase for cDNA synthesis, and producing single-stranded DNA by decomposition of the RNA of an RNA-DNA double strand by ribonuclease H; (b) using the single-stranded DNA as template for DNA-dependent DNA polymerase to form a double-stranded DNA with a promoter sequence for transcription of an RNA from the specific sequences or their complementary sequences and subsequently producing an RNA product in the presence of an RNA polym erase with the double-stranded DNA; (c) RNA amplification with such RNA transcription product as template for cDNA synthesis by the RNA-dependent DNA polymerase, wherein the first and second primers can also be those of not less than 10 bases long derived from sequences (XXVIII)-(XXXIII) all with 51 base pairs and from sequences (XXXIV)-(XLI) with 20-23 base pairs, identical to a part of the RNA sequence originating in the pab genes or their complementary sequences, for amplification.

    8.
    发明专利
    未知

    公开(公告)号：DE602004023462D1

    公开(公告)日：2009-11-19

    申请号：DE602004023462

    申请日：2004-08-31

    Applicant: TOSOH CORP

    Inventor： MASUDA NORIYOSHI ,  HORIE RYUICHI ,  YASUKAWA KIYOSHI

    IPC: C12N15/09 ,  C12Q1/68

    Abstract: A detection reagent for detecting thermostable direct hemolysin (TDH) gene in an amplification process which specifically amplifies TDH RNA, which reagent comprises a first primer having a sequence complementary to a specific sequence of an RNA derived from the TDH gene, and a second primer having a sequence homologous to said specific sequence.

    9.
    发明专利
    未知

    公开(公告)号：DE602005016313D1

    公开(公告)日：2009-10-08

    申请号：DE602005016313

    申请日：2005-02-02

    Applicant: TOSOH CORP

    Inventor： MASUDA NORIYOSHI ,  YASUKAWA KIYOSHI ,  HORIE RYUICHI

    IPC: C12Q1/68

    Abstract: The present invention provides a detection method for detecting norovirus, using an RNA amplification process comprising the steps of : producing a cDNA with an RNA-dependent DNA polymerase using a specific sequence of norovirus genome RNA as a template, as well as a specifically defined first primer having a sequence homologous to said specific sequence, and a specifically defined second primer having a sequence complementary to said specific sequence, thereby forming a double-strand RNA-DNA, wherein either the first primer or the second primer has a sequence in which a promoter sequence of an RNA polymerase has been added to its 5' end; degrading the RNA portion of said double-strand RNA-DNA by ribonuclease H, thereby producing a single-strand DNA; and producing a double-strand DNA having said promoter sequence capable of transcribing the RNA composed of the specific sequence of the RNA or the sequence complementary to said specific sequence of the RNA with a DNA-dependent DNA polymerase using said single-strand DNA as a template; wherein, the double-strand DNA produces an RNA transcription product in the presence of the RNA polymerase, and said RNA transcription product serves as a template for the subsequent cDNA synthesis with the RNA-dependent DNA polymerase.

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