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公开(公告)号：EP3286204A1
公开(公告)日：2018-02-28
申请号：EP16719824
申请日：2016-04-22
Applicant: UCB BIOPHARMA SPRL
Inventor： HEYWOOD SAM PHILIP , WILD GAVIN BARRY , HANIF RAZWAN , LE PAGE CHRISTOPHER JOHN
CPC classification number: C07K1/1136 , A61K39/39591 , C07K1/1133 , C07K16/18 , C07K16/2878 , C07K2317/31
Abstract: The present invention provides method of increasing the percentage of monomer in a composition of recombinantly expressed antibody molecules characterised in that the antibody molecule comprises at least one Fv with specificity for an antigen of interest comprising one VH and one VL wherein said VH and VL are connected directly or indirectly via one or more linkers and are stabilised by a disulfide bond therebetween, said method comprises: a) a conversion step of treating the composition with a denaturant selected from urea and/or Guanidine hydrochloride; b) wherein step a) is performed in the presence of a reducing agent or after treatment with a reducing agent.
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2.发明公开
公开(公告)号：EP3286220A1
公开(公告)日：2018-02-28
申请号：EP16721103
申请日：2016-04-22
Applicant: UCB BIOPHARMA SPRL
Inventor： HEYWOOD SAM PHILIP , WILD GAVIN BARRY , LE PAGE CHRISTOPHER JOHN , HANIF RAZWAN
CPC classification number: C07K16/2878 , C07K16/18 , C07K16/468 , C07K2317/14 , C07K2317/31 , C07K2317/55 , C07K2317/624 , C07K2317/66
Abstract: The present invention provides method for increasing the percentage of monomer in a composition of recombinantly expressed antibody molecules characterised in that the antibody molecule comprises at least one Fv with specificity for an antigen of interest comprising one VH and one VL wherein said VH and VL are connected directly or indirectly via one or more linkers and are stabilised by a disulfide bond therebetween, said method comprises: a)a thermal conversion stepof holding the composition comprising the antibody molecule at a temperature in the range 30 to 60°C for a period of at least 1 hour, wherein step a) is performed in the presence of a reducing agent or after treatment with a reducing agent.
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公开(公告)号：SG11201708151TA
公开(公告)日：2017-11-29
申请号：SG11201708151T
申请日：2016-04-22
Applicant: UCB BIOPHARMA SPRL
Inventor： HEYWOOD SAM PHILIP , WILD GAVIN BARRY , HANIF RAZWAN , LE PAGE CHRISTOPHER JOHN
Abstract: The present invention provides method of increasing the percentage of monomer in a composition of recombinantly expressed antibody molecules characterised in that the antibody molecule comprises at least one Fv with specificity for an antigen of interest comprising one VH and one VL wherein said VH and VL are connected directly or indirectly via one or more linkers and are stabilised by a disulfide bond therebetween, said method comprises: a) a conversion step of treating the composition with a denaturant selected from urea and/or Guanidine hydrochloride; b) wherein step a) is performed in the presence of a reducing agent or after treatment with a reducing agent.
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4.发明专利
公开(公告)号：CL2017002677A1
公开(公告)日：2018-03-23
申请号：CL2017002677
申请日：2017-10-20
Applicant: UCB BIOPHARMA SPRL
Inventor： HEYWOOD SAM PHILIP , WILD GAVIN BARRY , HANIF RAZWAN , LE PAGE CHRISTOPHER JOHN
Abstract: LA PRESENTE INVENCIÓN PROPORCIONA UN MÉTODO PARA AUMENTAR EL PORCENTAJE DE MONÓMERO EN UNA COMPOSICIÓN DE MOLÉCULAS DE ANTICUERPO EXPRESADAS RECOMBINAITEMENTE QUE SE CARACTERIZAN PORQUE LA MOLÉCULA DE ANTICUERPO COMPRENDE AL MENOS UN EV CON ESPECIFICIDAD PARA UN ANTÍGENO DE INTERÉS QUE COMPRENDE UNA VH Y UNA VL EN LA QUE DICHOS VH Y VL ESTÁN CONECTADOS DIRECTA O INDIRECTAMENTE A TRAVÉS DE UNO O MÁS ENLACES Y SE ESTABILIZAN MEDIANTE UN ENLACE DISULFURO ENTRE ELLOS, DICHO MÉTODO COMPRENDE: A) UNA ETAPA DE CONVERSIÓN TÉRMICA DE MANTENER LA COMPOSICIÓN QUE COMPRENDE LA MOLÉCULA DE ANTICUERPO A UNA TEMPERATURA EN EL INTERVALO DE 30 A 60 C DURANTE UN PERÍODO DE AL MENOS 1 HORA, EN EL QUE LA ETAPA A) SE REALIZA EN LA PRESENCIA DE UN AGENTE REDUCTOR O DESPUÉS DEL TRATAMIENTO CON UN AGENTE REDUCTOR.
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公开(公告)号：AU2016251620A1
公开(公告)日：2017-10-26
申请号：AU2016251620
申请日：2016-04-22
Applicant: UCB BIOPHARMA SPRL
Inventor： HEYWOOD SAM PHILIP , WILD GAVIN BARRY , HANIF RAZWAN , LE PAGE CHRISTOPHER JOHN
Abstract: The present invention provides method of increasing the percentage of monomer in a composition of recombinantly expressed antibody molecules characterised in that the antibody molecule comprises at least one Fv with specificity for an antigen of interest comprising one VH and one VL wherein said VH and VL are connected directly or indirectly via one or more linkers and are stabilised by a disulfide bond therebetween, said method comprises: a) a conversion step of treating the composition with a denaturant selected from urea and/or Guanidine hydrochloride; b) wherein step a) is performed in the presence of a reducing agent or after treatment with a reducing agent.
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Patent Agency Ranking
公开(公告)号：CO2017010385A2
公开(公告)日：2018-03-20
申请号：CO2017010385
申请日：2017-10-11
Applicant: UCB BIOPHARMA SPRL
Inventor： HEYWOOD SAM PHILIP , WILD GAVIN BARRY , HANIF RAZWAN , LE PAGE CHRISTOPHER JOHN
Abstract: La presente invención proporciona un método para aumentar el porcentaje de monómero en una composición de moléculas de anticuerpo expresadas de forma recombinante, caracterizado porque la molécula de anticuerpo comprende al menos un Fv con especificidad para un antígeno de interés que comprende un VH y un VL, en donde dichos VH y VL se conectan directa o indirectamente a través de uno o más enlazadores y se estabilizan mediante un enlace disulfuro entre ellos, dicho método comprende: a) una etapa de conversión para tratar la composición con un desnaturalizante seleccionado a partir de urea y/o clorhidrato de guanidina; b) en donde la etapa a) se lleva a cabo en presencia de un agente reductor o después del tratamiento con un agente reductor.
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公开(公告)号：CO2017010845A2
公开(公告)日：2018-03-20
申请号：CO2017010845
申请日：2017-10-25
Applicant: UCB BIOPHARMA SPRL
Inventor： HEYWOOD SAM PHILIP , WILD GAVIN BARRY , HANIF RAZWAN , LE PAGE CHRISTOPHER JOHN
Abstract: RESUMEN La presente invención proporciona un método para aumentar el porcentaje del monómero en una composición de moléculas del anticuerpo expresadas de manera recombinante, caracterizado porque la molécula del anticuerpo comprende al menos un Fv con especificidad hacia un antígeno de interés que comprende un VH y un VL, en donde el VH y el VL están conectados directa o indirectamente por medio de uno o más enlazadores y son estabilizados por medio de un enlace de disulfuro entre los mismos, el método comprende: a) una etapa de conversión térmica manteniendo la composición que comprende la molécula del anticuerpo a una temperatura en el intervalo de 30 a 60 °C durante un periodo de al menos 1 hora, en donde la etapa a) es efectuada en la presencia de un agente reductor o después del tratamiento con un agente reductor.
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公开(公告)号：SG11201708430VA
公开(公告)日：2017-11-29
申请号：SG11201708430V
申请日：2016-04-22
Applicant: UCB BIOPHARMA SPRL
Inventor： HEYWOOD SAM PHILIP , WILD GAVIN BARRY , LE PAGE CHRISTOPHER JOHN , HANIF RAZWAN
Abstract: The present invention provides method for increasing the percentage of monomer in a composition of recombinantly expressed antibody molecules characterised in that the antibody molecule comprises at least one Fv with specificity for an antigen of interest comprising one VH and one VL wherein said VH and VL are connected directly or indirectly via one or more linkers and are stabilised by a disulfide bond therebetween, said method comprises a) a thermal conversion step of holding the composition comprising the antibody molecule at a temperature in the range 30 to 60° C. for a period of at least 1 hour, wherein step a) is performed in the presence of a reducing agent or after treatment with a reducing agent.
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公开(公告)号：AU2016251960A1
公开(公告)日：2017-11-02
申请号：AU2016251960
申请日：2016-04-22
Applicant: UCB BIOPHARMA SPRL
Inventor： HEYWOOD SAM PHILIP , WILD GAVIN BARRY , LE PAGE CHRISTOPHER JOHN , HANIF RAZWAN
Abstract: The present invention provides method for increasing the percentage of monomer in a composition of recombinantly expressed antibody molecules characterised in that the antibody molecule comprises at least one Fv with specificity for an antigen of interest comprising one VH and one VL wherein said VH and VL are connected directly or indirectly via one or more linkers and are stabilised by a disulfide bond therebetween, said method comprises: a)a thermal conversion stepof holding the composition comprising the antibody molecule at a temperature in the range 30 to 60°C for a period of at least 1 hour, wherein step a) is performed in the presence of a reducing agent or after treatment with a reducing agent.
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公开(公告)号：CA2983537A1
公开(公告)日：2016-10-27
申请号：CA2983537
申请日：2016-04-22
Applicant: UCB BIOPHARMA SPRL
Inventor： HEYWOOD SAM PHILIP , WILD GAVIN BARRY , LE PAGE CHRISTOPHER JOHN , HANIF RAZWAN
Abstract: The present invention provides method for increasing the percentage of monomer in a composition of recombinantly expressed antibody molecules characterised in that the antibody molecule comprises at least one Fv with specificity for an antigen of interest comprising one VH and one VL wherein said VH and VL are connected directly or indirectly via one or more linkers and are stabilised by a disulfide bond therebetween, said method comprises: a)a thermal conversion stepof holding the composition comprising the antibody molecule at a temperature in the range 30 to 60°C for a period of at least 1 hour, wherein step a) is performed in the presence of a reducing agent or after treatment with a reducing agent.
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