公开(公告)号：AU2016327573B2

公开(公告)日：2022-07-14

申请号：AU2016327573

申请日：2016-09-22

Applicant: UNIV BOSTON

Inventor： UNLU SELIM ,  DAABOUL GEORGE G ,  CHIARI MARCELLA

IPC: G01N21/00 ,  G01N21/75 ,  G01N21/77 ,  G01N33/00 ,  G01N33/48

Abstract: Provided herein are methods for capturing extracellular vesicles from a biological sample for quantification and/or characterization (e.g., size and/or shape discrimination) using an SP-IRIS system. Also provided herein are methods of detecting a biomarker on captured extracellular vesicles or inside the captured vesicles (e.g., intra-vesicular or intra-exosomal biomarkers).

公开(公告)号：AU2016327573A1

公开(公告)日：2018-04-12

申请号：AU2016327573

申请日：2016-09-22

Applicant: UNIV BOSTON

Inventor： UNLU SELIM ,  DAABOUL GEORGE G ,  CHIARI MARCELLA

IPC: G01N21/00 ,  G01N21/75 ,  G01N21/77 ,  G01N33/00 ,  G01N33/48

Abstract: Provided herein are methods for capturing extracellular vesicles from a biological sample for quantification and/or characterization (e.g., size and/or shape discrimination) using an SP-IRIS system. Also provided herein are methods of detecting a biomarker on captured extracellular vesicles or inside the captured vesicles (e.g., intra-vesicular or intra-exosomal biomarkers).

公开(公告)号：EP3114429A4

公开(公告)日：2017-11-08

申请号：EP15757686

申请日：2015-03-06

Applicant: UNIV BOSTON

Inventor： UNLU SELIM ,  DAABOUL GEORGE ,  YURT ABDULKADIR

IPC: G01B9/02 ,  G01B9/04 ,  G01N21/21 ,  G01N21/45 ,  G01N21/55

CPC classification number: G01N21/55 ,  G01B9/02011 ,  G01N21/21 ,  G01N21/45 ,  G01N21/554 ,  G01N2021/216 ,  G01N2201/06113 ,  G01N2201/0683

Abstract: An imaging system uses polarized light to illuminate the target and then uses a polarization filter to remove the light that is reflected from the target without modification. The target can include one or more anisotropic objects that scatter the light and alter the polarization state of the reflected light and causing it to be selectively transmitted to the imaging device which can record the transmitted light through the filter. The illuminating light can be circularly polarized and the filter can remove the circularly polarized light. The target can include asymmetric nanoparticles, such as nanorods that alter the amplitude or phase of the scattered light enabling pass through the filter to be detected by the imaging device.