公开(公告)号：AU2011305983B2

公开(公告)日：2014-06-12

申请号：AU2011305983

申请日：2011-06-21

Applicant: UNIV CALIFORNIA

Inventor： JALALI BAHRAM ,  MOTAFAKKER-FARD ALI

IPC: G01B9/02 ,  G01B11/24

Abstract: We describe methods and apparatus for high-speed high-contrast imaging one-, two- and three-dimensional imaging enabled by differential interference contrast time encoded amplified microscopy of transparent media without the need for chemical staining, that are suitable for a broad range of applications from semiconductor process monitoring to blood screening. Our methods and apparatus build on a unique combination of serial time-encoded amplified microscopy (STEAM) and differential interference contrast (DIC) microscopy. These methods and apparatus are ideally suited for identification of rare diseased cells in a large population of healthy cells and have the potential to revolutionize blood analysis and pathology including identification of cancer cells, such as Circulating Tumor Cells (CTC) in early stage disease.

公开(公告)号：EP2321766A4

公开(公告)日：2014-01-15

申请号：EP09801032

申请日：2009-07-23

Applicant: UNIV CALIFORNIA

Inventor： GODA KEISUKE ,  JALALI BAHRAM ,  TSIA KEVIN KIN-MAN

IPC: G06K9/20 ,  G06K7/10 ,  G06K7/14

CPC classification number: G06K7/1439 ,  G06K7/10712 ,  G06K7/10831 ,  G06K7/14

公开(公告)号：EP2583053A4

公开(公告)日：2013-11-20

申请号：EP11827118

申请日：2011-06-21

Applicant: UNIV CALIFORNIA

Inventor： JALALI BAHRAM ,  MOTAFAKKER-FARD ALI

IPC: G01B9/02 ,  G01B11/24 ,  G01J1/04 ,  G02B21/00 ,  G02B21/14 ,  H04N5/14

CPC classification number: G01J1/0411 ,  G02B21/002 ,  G02B21/14 ,  H04N5/14

Abstract: We describe methods and apparatus for high-speed high-contrast imaging one-, two- and three-dimensional imaging enabled by differential interference contrast time encoded amplified microscopy of transparent media without the need for chemical staining, that are suitable for a broad range of applications from semiconductor process monitoring to blood screening. Our methods and apparatus build on a unique combination of serial time-encoded amplified microscopy (STEAM) and differential interference contrast (DIC) microscopy. These methods and apparatus are ideally suited for identification of rare diseased cells in a large population of healthy cells and have the potential to revolutionize blood analysis and pathology including identification of cancer cells, such as Circulating Tumor Cells (CTC) in early stage disease.

公开(公告)号：ES2845600T3

公开(公告)日：2021-07-27

申请号：ES14737736

申请日：2014-01-09

Applicant: UNIV CALIFORNIA

Inventor： DIEBOLD ERI D ,  JALALI BAHRAM ,  BUCKLEY BRANDON

IPC: G01N21/64 ,  G01B9/02 ,  G02B21/16 ,  G02F1/11

Abstract: Un método de obtención de imágenes por fluorescencia, comprendiendo el método: con un deflector acústico-óptico (22) accionado por un peine de RF (20) y un rayo láser de excitación (16), crear (82) una pluralidad de primeros haces (24) de luz con un rango de ángulos de salida y cambios de frecuencia; con un variador de frecuencia acústico-óptico (28) accionado por un tono de RF y un rayo láser (18) de excitación, crear (84) un segundo haz (30) de luz desplazado en frecuencia; combinar la pluralidad de los primeros haces (24) y el segundo haz (30) para producir una pluralidad de haces de luz (34) combinados desplazados en frecuencia; interrogar (86) simultáneamente a múltiples puntos en una muestra (46) con la pluralidad de haces de luz (34) combinados; y detectar (88) una respuesta fluorescente de la muestra por exposición a la pluralidad de haces de luz combinados.

公开(公告)号：ES2537333T3

公开(公告)日：2015-06-05

申请号：ES09801032

申请日：2009-07-23

Applicant: UNIV CALIFORNIA

Inventor： GODA KEISUKE ,  JALALI BAHRAM ,  TSIA KEVIN KIN-MAN

IPC: G06V30/224 ,  G06K7/10 ,  G06K7/14

Abstract: Un aparato (20, 52, 88, 124, 170, 210, 250, 270) para leer patrones y detectar desplazamientos, que comprende: un medio (22, 54, 90, 126) para generar un haz de sonda pulsado de banda ancha (178); un medio (34, 66, 108, 144, 262, 340) para correlacionar mediante la transformada de Fourier del espectro de dicho haz de sonda pulsado con un dominio espacial para formar un haz óptico dispersado espacialmente (182) dirigido a un objetivo (38, 70, 110, 146, 214), en el que dicho objetivo (38, 70, 110, 146, 214) ha de ser leído; un medio (34, 66, 108, 144, 262, 34b) para formar una señal óptica de respuesta basándose en la energía óptica procedente del objetivo; un medio (40, 72, 112, 148) para la transformada de Fourier dispersiva de dicha señal óptica de respuesta para convertir dicha señal óptica de respuesta en una forma de onda en el dominio del tiempo; y un medio (42, 200, 78, 114, 154) para detectar y analizar dicha forma de onda en el dominio del tiempo para determinar patrones y/o desplazamientos dentro del objetivo que es leído.

公开(公告)号：WO2007112449A3

公开(公告)日：2008-08-07

申请号：PCT/US2007065393

申请日：2007-03-28

Applicant: UNIV CALIFORNIA ,  SOLLI DANIEL ,  CHOU JASON ,  JALALI BAHRAM

Inventor： SOLLI DANIEL ,  CHOU JASON ,  JALALI BAHRAM

IPC: G01J3/44

CPC classification number: G01J3/4412 ,  G01J3/10 ,  G01J3/457 ,  G01N21/636 ,  G01N21/65 ,  G01N21/658 ,  G01N2021/653 ,  G01N2021/655

Abstract: An apparatus and method for measuring Raman-type spectra using optical dispersion to convert an optical spectrum into a waveform which can be detected directly in the time domain without the use of a conventional spectrometer. In the example of stimulated Raman spectroscopy, the apparatus and method exposes a sample to a chirped, pulsed probe beam and a Raman pump beam and the resulting Raman spectra is detected by an optical detector in the time domain, and analyzed. Alternatively, the Raman spectra from the probe and pump beams is chirped with a dispersive element prior to detection and analysis. Each probe pulse provides a snapshot of the Raman spectrum that is sampled in time so that neither repetitive waveforms nor static samples are required. Therefore, high speed acquisitions and high throughput assays can be conducted. To facilitate detection, these spectral signals can also be amplified using distributed Raman amplification directly in the dispersive element.

Abstract translation: 一种使用光学色散测量拉曼光谱的装置和方法，用于将光谱转换成可在时域中直接检测的波形，而不需要使用常规光谱仪。 在受激拉曼光谱的实例中，该装置和方法将样品暴露于啁啾脉冲探测光束和拉曼泵浦光束，并且通过时域中的光学检测器检测所得拉曼光谱，并进行分析。 或者，来自探针和泵浦光束的拉曼光谱在检测和分析之前用分散元件啁啾。 每个探针脉冲提供了在时间上采样的拉曼光谱的快照，以便不需要重复波形和静态样本。 因此，可以进行高速采集和高通量测定。 为了便于检测，这些光谱信号也可以使用分散拉曼放大直接在色散元件中进行扩增。

公开(公告)号：WO2006033853A3

公开(公告)日：2007-06-07

申请号：PCT/US2005032126

申请日：2005-09-07

Applicant: UNIV CALIFORNIA ,  JALALI BAHRAM ,  SIENICKI TODD

Inventor： JALALI BAHRAM ,  SIENICKI TODD

IPC: G02B6/32 ,  G02B6/26 ,  G02B6/42

CPC classification number: G02B6/29392 ,  G02B6/29311 ,  G02B6/29314 ,  H04B10/25133

Abstract: A system and method to chromomodally generate dispersion in light waves. The system and method may be used to control dispersive effects of an optical element such as a single-mode fiber. A light beam from the optical element is first collimated and then directed on to a spatially diffractive element where it is spatially dispersed into various chromatic frequency components. This frequency-separated light is then imparted onto a dispersion slope equalizer, and then passed into a highly multimode waveguide, where it is further dispersed. The light is then collected and focused back into an outgoing fiber-optic or other optical device.

Abstract translation: 在光波中色散地产生色散的系统和方法。 该系统和方法可用于控制诸如单模光纤的光学元件的色散效应。 来自光学元件的光束首先被准直，然后被引导到空间衍射元件，其中它被空间分散成各种彩色频率分量。 然后将该频率分离的光赋予色散斜率均衡器，然后传递到高度多模波导中，在此进一步分散。 然后将光收集并聚焦回出射光纤或其它光学装置。

公开(公告)号：EP3120130A4

公开(公告)日：2017-11-08

申请号：EP15764027

申请日：2015-03-18

Applicant: UNIV CALIFORNIA

Inventor： JALALI BAHRAM ,  DIEBOLD ERIC ,  BUCKLEY BRANDON

IPC: G01N15/14 ,  G01N15/10 ,  G01N21/64 ,  G01N33/48 ,  G01N33/537

CPC classification number: G01N15/1434 ,  G01N15/1459 ,  G01N15/1484 ,  G01N21/64 ,  G01N21/6428 ,  G01N21/6458 ,  G01N21/6486 ,  G01N33/537 ,  G01N2015/1006 ,  G01N2015/1477 ,  G01N2021/6421 ,  G01N2201/067

Abstract: An imaging flow cytometry apparatus and method which allows registering multiple locations across a cell, and/or across multiple flow channels, in parallel using radio-frequency-tagged emission (FIRE) coupled with a parallel optical detection scheme toward increasing analysis throughput. An optical source is modulated by multiple RF frequencies to produce an optical interrogation beam having a spatially distributed beat frequency. This beam is directed to one or more focused streams of cells whose responsive fluorescence, in different frequencies, is registered in parallel by an optical detector.

公开(公告)号：EP2943778A4

公开(公告)日：2016-09-28

申请号：EP14737736

申请日：2014-01-09

Applicant: UNIV CALIFORNIA

Inventor： DIEBOLD ERI D ,  JALALI BAHRAM ,  BUCKLEY BRANDON

IPC: G01N21/64 ,  G01B9/02

CPC classification number: G01N21/6408 ,  G01N21/05 ,  G01N21/6458 ,  G01N21/6486 ,  G01N2021/6415 ,  G01N2021/6419 ,  G01N2201/06113 ,  G02B21/0076 ,  G02B21/0084 ,  G02B21/16 ,  G02B27/1006 ,  G02F1/11

Abstract: Apparatus and methods for fluorescence imaging using radiofrequency multiplexed excitation. One apparatus splits an excitation laser beam into two arms of a Mach-Zehnder interferometer. The light in the first beam is frequency shifted by an acousto-optic deflector, which is driven by a phase-engineered radiofrequency comb designed to minimize peak-to-average power ratio. This RF comb generates multiple deflected optical beams possessing a range of output angles and frequency shifts. The second beam is shifted in frequency using an acousto-optic frequency shifter. After combining at a second beam splitter, the two beams are focused to a line on the sample using a conventional laser scanning microscope lens system. The acousto-optic deflectors frequency-encode the simultaneous excitation of an entire row of pixels, which enables detection and de-multiplexing of fluorescence images using a single photomultiplier tube and digital phase-coherent signal recovery techniques.

Abstract translation: 使用射频多路复用荧光成像的装置和方法。 一个装置将激发激光束分成Mach-Zehnder干涉仪的两个臂。 第一光束中的光被声光偏转器偏移，声光偏转器由相位设计的射频梳驱动，旨在最小化峰值与平均功率比。 该RF梳产生具有输出角和频移范围的多个偏转光束。 第二个波束使用声光移频器在频率上移动。 在第二分束器上组合之后，使用常规的激光扫描显微镜透镜系统将两个光束聚焦到样品上的线上。 声光偏转器对整行像素的同时激励进行频率编码，这使得能够使用单个光电倍增管和数字相位相干信号恢复技术来检测和解复用荧光图像。

公开(公告)号：WO2010059993A3

公开(公告)日：2010-09-10

申请号：PCT/US2009065411

申请日：2009-11-20

Applicant: UNIV CALIFORNIA ,  GUPTA SHALABH ,  JALALI BAHRAM ,  MOTAFAKKER-FARD ALI

Inventor： GUPTA SHALABH ,  JALALI BAHRAM ,  MOTAFAKKER-FARD ALI

IPC: H03M1/12

CPC classification number: G01R13/0218 ,  G02F1/225 ,  G02F7/00 ,  H03M1/1042 ,  H03M1/12

Abstract: A time-stretched enhanced recording scope (TiSER) is described using time stretch analog-to-digital conversion in a real-time burst mode. A chirped optical signal is modulated in response to receiving segments of an input signal. The optical signal with its modulated input signal, is stretched through an optical medium and digitized to represent the waveform segment. TiSER provides ultra-fast real-time sampling within short segment bursts of the original input signal while providing an ability to detect non-repetitive events. Methods and apparatus are also described for providing real-time information about inter-symbol information (ISI), rapidly determining bit-error rates (BER), performing time-domain reflectometry (TDR), generating eye diagrams for serial data, facilitating digital correction of data, clock recovery, optical carrier phase recovery, and otherwise increasing the speed and/or accuracy of a diverse range of high-speed signal measurement and processing activities.

Abstract translation: 使用实时突发模式中的时间拉伸模数转换来描述时间延长的增强记录范围（TiSER）。 响应于输入信号的接收段调制啁啾光信号。 具有调制输入信号的光信号通过光学介质被拉伸并数字化以表示波形段。 TiSER在原始输入信号的短片段突发中提供超快速实时采样，同时提供检测非重复事件的能力。 还描述了用于提供关于符号间信息（ISI），快速确定误码率（BER），执行时域反射计（TDR），生成串行数据眼图，促进数字校正的实时信息的方法和装置 的数据，时钟恢复，光载波相位恢复，否则增加了各种范围的高速信号测量和处理活动的速度和/或精度。