公开(公告)号：ES2831400T3

公开(公告)日：2021-06-08

申请号：ES14754767

申请日：2014-02-20

Applicant: UNIV HEALTH NETWORK

Inventor： KESHAVJEE SHAF ,  LIU MINGYAO ,  CYPEL MARCELO

IPC: G01N33/68

Abstract: Método para clasificar un injerto pulmonar sometido a perfusión pulmonar ex vivo (PPEV) normotérmica, durante la perfusión y/o después de la perfusión, comprendiendo el método: a) recoger una muestra de ensayo del injerto pulmonar, en donde el injerto pulmonar es de un donante en muerte cardíaca (DMC); b) medir un nivel de polipéptido de un producto génico predictor de endotelina seleccionado de endotelina 1 (ET-1) y ET-1 grande en la muestra de ensayo; e c) identificar el injerto pulmonar como un buen candidato para trasplante o como un mal candidato para trasplante, en donde un nivel de polipéptido aumentado del producto génico predictor de endotelina en comparación con un control de pronóstico es indicativo de que el injerto es un mal candidato para trasplante.

公开(公告)号：CA2938893A1

公开(公告)日：2014-08-28

申请号：CA2938893

申请日：2014-02-20

Applicant: UNIV HEALTH NETWORK

Inventor： KESHAVJEE SHAF ,  LIU MINGYAO ,  CYPEL MARCELO

IPC: C12Q1/68 ,  C12Q1/6809 ,  G01N33/48 ,  G01N33/53 ,  G06F19/20 ,  G16B25/10

Abstract: Methods for assaying a donor lung for chronic allograft lung dysfunction (CLAD) optionally bronchiolitis obliterans syndrome (BOS) subtype or restrictive allograft syndrome (RAS) subtype of CLAD or risk of developing BOS subtype or RAS subtype CLAD post-transplant, the method comprising: a. measuring a normalized expression level of an RNA transcript of IL-6 or an expression product thereof in a sample of the donor lung pre-transplant or a normalized expression level of one or more S100 protein, optionally S100A8 and/or S100A9, polypeptide expression product in a sample from the donor lung post-transplant; b. assessing the likelihood of the donor lung developing BOS subtype CLAD or RAS subtype CLAD post-transplant based on said IL-6, S100, optionally S100A8 and/or S100A9, expression level wherein IL-6 expression level is positively correlated with an increased likelihood of developing BOS post-transplant, S100A8 expression level is positively correlated with having or having an increased likelihood of developing RAS and/or BOS subtype CLAD, and S100A9 is positively correlated with having and having an increased likelihood of developing RAS subtype CLAD.

公开(公告)号：CA2935866A1

公开(公告)日：2014-08-28

申请号：CA2935866

申请日：2014-02-20

Applicant: UNIV HEALTH NETWORK

Inventor： KESHAVJEE SHAF ,  LIU MINGYAO ,  CYPEL MARCELO

IPC: G01N33/48 ,  G01N33/483 ,  G01N33/53 ,  G01N33/543

Abstract: A method of classifying a lung graft subjected to normothermic ex vivo lung perfusion (EVLP), during perfusion and/or after perfusion, the method comprising: a) collecting a test sample from the lung graft; b) measuring a polypeptide level of a negative transplant predictor gene product selected from CCG predictor gene products M-CSF, IL-8 SCGF-beta, GRO-alpha, G-CSF, MIP-1 alpha, and/or MIP-1beta, endothelin predictor gene products endothelin 1 (ET-1) and/or big ET-1, and/or apoptosis predictor gene products cytokeratin 18 (CK-18), caspase 3 and/or HMGB-1 in the sample and/or determining a metabolite profile of the sample for lung grafts that are from donors where the death was due to cardiac death (DCD); c) identifying the graft as a good candidate for transplant or a poor candidate for transplant wherein an increased polypeptide level of one or more negative transplant outcome predictor gene products compared to an outcome control or a reference metabolic profile is indicative the graft is a poor candidate for transplant.

公开(公告)号：EP2959292A4

公开(公告)日：2016-12-14

申请号：EP14754767

申请日：2014-02-20

Applicant: UNIV HEALTH NETWORK

Inventor： KESHAVJEE SHAF ,  LIU MINGYAO ,  CYPEL MARCELO

IPC: G01N33/68 ,  C40B30/02

CPC classification number: G01N33/6884 ,  C40B30/02 ,  G01N33/68 ,  G01N2333/5754 ,  G01N2800/245

Abstract: A method of classifying a lung graft subjected to normothermic ex vivo lung perfusion (EVLP), during perfusion and/or after perfusion, the method comprising: a) collecting a test sample from the lung graft; b) measuring a polypeptide level of a negative transplant predictor gene product selected from CCG predictor gene products M-CSF, IL-8 SCGF-beta, GRO-alpha, G-CSF, MIP-1 alpha, and/or MIP-1beta, endothelin predictor gene products endothelin 1 (ET-1) and/or big ET-1, and/or apoptosis predictor gene products cytokeratin 18 (CK-18), caspase 3 and/or HMGB-1 in the sample and/or determining a metabolite profile of the sample for lung grafts that are from donors where the death was due to cardiac death (DCD); c) identifying the graft as a good candidate for transplant or a poor candidate for transplant wherein an increased polypeptide level of one or more negative transplant outcome predictor gene products compared to an outcome control or a reference metabolic profile is indicative the graft is a poor candidate for transplant.

公开(公告)号：AU2018247584A1

公开(公告)日：2019-11-21

申请号：AU2018247584

申请日：2018-03-29

Applicant: UNIV DE SAO PAULO ,  UNIV HEALTH NETWORK

Inventor： BAGNATO VANDERLEI SALVADOR ,  CYPEL MARCELO ,  KESHAVJEE SHAFIQUE ,  WADDELL THOMAS KENNETH ,  GALASSO MARCOS THEOPHILO

IPC: A61L2/08 ,  A61L2/10 ,  B01L3/00

Abstract: Disclosed are apparatuses and methods for irradiating a perfusate. The apparatus includes a tank which defines a first chamber. A separator is located inside the first chamber. The separator defines a second chamber. The first chamber and the second chamber are concentric and have substantially annular cross sections, each having at least one diameter and a substantially common longitudinal axis. A perfusate is introduced into the first chamber by an inlet. A UV radiation-emitting device is disposed inside the second chamber for providing irradiation to the perfusate. Irradiated perfusate is removed from the tank by an outlet. Other apparatuses and systems are described and methods for inactivating micro organisms by performing EVP and irradiating the perfusate.

公开(公告)号：CA3070522A1

公开(公告)日：2019-02-07

申请号：CA3070522

申请日：2018-07-30

Applicant: UNIV HEALTH NETWORK

Inventor： KESHAVJEE SHAFIQUE ,  LIU MINGYAO ,  CYPEL MARCELO ,  SAGE ANDREW

IPC: G01N33/48 ,  A61B5/00 ,  G01N33/53 ,  G01N33/543

Abstract: Methods and kits for screening, diagnosing, detecting or predicting a patient outcome/risk variable for a lung transplant recipient after transplant or an EVLP outcome by measuring biomarker levels of one or more biomarkers selected from IL-6, IL-8, sTNFR1 and sTREM-1 in EVLP perfusate are described. The methods involve for example, i. obtaining one or more test EVLP perfusate samples of a donor lung; ii. determining in one or more test EVLP perfusate sample of a donor lung, a polypeptide level of one or more biomarkers selected from IL-8, IL-6, sTNFR1 and sTREM-1; and iii. a) comparing the polypeptide level of the one or more biomarkers in the perfusate sample with a control or cut-off level, wherein the differential level is indicative of outcome/risk of after transplant or of an EVLP outcome; or b) using the polypeptide level of one or several of the one or more biomarkers in combination, as part of an algebraic calculation of outcome/risk.

公开(公告)号：CA2944922A1

公开(公告)日：2015-10-15

申请号：CA2944922

申请日：2015-04-10

Applicant: UNIV HEALTH NETWORK

Inventor： HARRISON DANIEL GAVIN ,  FROST GEOFFREY SAMUEL ,  WADDELL THOMAS KENNETH ,  WYVILL LAURA ANN ,  CRACAUER RAYMOND FRANCIS ,  KESHAVJEE SHAFIQUE ,  CYPEL MARCELO ,  YANG RANDY

IPC: A61B17/34 ,  A61M39/02

Abstract: A cannula for connecting a medical device to a biological system is taught. The cannula includes a tissue engagement portion, preferably in the form of an annulus, to which a vacuum is applied through the cannula to attract and hold tissue of the biological system in an initial connection while an affixment device is applied to complete the connection. In addition to a working fluid conduit, comprising a main port, a working fluid passage and a working fluid port, and a port to apply the vacuum, the cannula can include a sensor port to allow sensing pressure or other characteristics of the working fluid at a point closely adjacent to the connection between the cannula and the biological system.

公开(公告)号：ES2991992T3

公开(公告)日：2024-12-05

申请号：ES15776037

申请日：2015-04-10

Applicant: UNIV HEALTH NETWORK

Inventor： HARRISON DANIEL GAVIN ,  FROST GEOFFREY SAMUEL ,  WADDELL THOMAS KENNETH ,  WYVILL LAURA ANN ,  CRACAUER RAYMOND FRANCIS ,  KESHAVJEE SHAFIQUE ,  CYPEL MARCELO ,  YANG RANDY

IPC: A61B17/34 ,  A61M39/02 ,  A61N1/02

Abstract: Se enseña una cánula para conectar un dispositivo médico a un sistema biológico. La cánula incluye una porción de acoplamiento de tejido, preferiblemente en forma de anillo, a la que se aplica un vacío a través de la cánula para atraer y mantener el tejido del sistema biológico en una conexión inicial mientras se aplica un dispositivo de fijación para completar la conexión. Además de un conducto de fluido de trabajo, que comprende un puerto principal, un paso de fluido de trabajo y un puerto de fluido de trabajo, y un puerto para aplicar el vacío, la cánula puede incluir un puerto de sensor para permitir detectar la presión u otras características del fluido de trabajo en un punto muy adyacente a la conexión entre la cánula y el sistema biológico. (Traducción automática con Google Translate, sin valor legal)

公开(公告)号：CA2938893C

公开(公告)日：2022-12-13

申请号：CA2938893

申请日：2014-02-20

Applicant: UNIV HEALTH NETWORK

Inventor： KESHAVJEE SHAF ,  LIU MINGYAO ,  CYPEL MARCELO

IPC: C12Q1/6809 ,  C12Q1/68 ,  G01N33/48 ,  G01N33/53 ,  G16B25/10

Abstract: Methods for assaying a donor lung for chronic allograft lung dysfunction (CLAD) optionally bronchiolitis obliterans syndrome (BOS) subtype or restrictive allograft syndrome (RAS) subtype of CLAD or risk of developing BOS subtype or RAS subtype CLAD post-transplant, the method comprising: a. measuring a normalized expression level of an RNA transcript of IL-6 or an expression product thereof in a sample of the donor lung pre-transplant or a normalized expression level of one or more S100 protein, optionally S100A8 and/or S100A9, polypeptide expression product in a sample from the donor lung post-transplant; b. assessing the likelihood of the donor lung developing BOS subtype CLAD or RAS subtype CLAD post-transplant based on said IL-6, S100, optionally S100A8 and/or S100A9, expression level wherein IL-6 expression level is positively correlated with an increased likelihood of developing BOS post-transplant, S100A8 expression level is positively correlated with having or having an increased likelihood of developing RAS and/or BOS subtype CLAD, and S100A9 is positively correlated with having and having an increased likelihood of developing RAS subtype CLAD.

公开(公告)号：CA3122341A1

公开(公告)日：2020-06-18

申请号：CA3122341

申请日：2019-12-13

Applicant: UNIV HEALTH NETWORK

Inventor： KESHAVJEE SHAFIQUE ,  LIU MINGYAO ,  CYPEL MARCELO ,  SAGE ANDREW

IPC: G01N33/48 ,  G01N33/53 ,  G01N33/543 ,  G06F16/90 ,  G06N20/00

Abstract: Methods and kits for screening, diagnosing, detecting or predicting a patient outcome/risk variable for a lung transplant recipient after transplant or an EVLP outcome by measuring biomarker levels of at least three biomarkers selected from IL-6, IL-8, IL-10 and IL-1ß optionally in combination with one or both of sTNFR1 and sTREM1 in EVLP perfusate are described. The methods involve for example, i. obtaining one or more test EVLP perfusate samples of a donor lung; ii. determining in one or more test EVLP perfusate sample of a donor lung, a polypeptide level of the at least three biomarkers selected from IL-8, IL-6, IL-10 and IL-1ß and optionally one or both of sTNFR1 and sTREM1 i; and iii. a) comparing the one or more parameter values related to a level of the at least three biomarkers in the perfusate sample with control EVLP data or a cut-off level, wherein the differential level is indicative of outcome/risk of after transplant or of an EVLP outcome; or b) using the one or more parameter values related to a level of the at least three biomarkers in combination, as part of an algebraic calculation or model of outcome/risk.