公开(公告)号：CA2901377A1

公开(公告)日：2014-08-21

申请号：CA2901377

申请日：2014-02-18

Applicant: HOSPITAL FOR SICK CHILDREN ,  UNIV HEALTH NETWORK

Inventor： KELLER GORDON ,  OGAWA SHINICHIRO ,  GHANEKAR ANAND ,  BEAR CHRISTINE ,  KAMATH BINITA M ,  OGAWA MINA ,  SURAPISITCHAT JAMES

IPC: C12N5/071 ,  A61K35/12 ,  A61K35/407 ,  A61P1/16 ,  C12N5/0735 ,  C12Q1/02

Abstract: Methods for producing hepatocyte and/or cholangiocyte lineage cells from pluripotent stem cells, the method comprising (a) specifying the extended nodal agonist treated induced endodermal cell population to obtain a cell population comprising hepatocyte and/or cholangiocyte progenitors by contacting the extended nodal agonist treated induced endodermal cell population with specification media comprising a FGF agonist and a BMP4 agonist and/or active conjugates and/or fragments thereof; (b) inducing maturation, and optionally further lineage specification and/or expansion of the hepatocyte and/or cholangiocyte progenitors of the cell population to obtain a population comprising hepatocyte lineage cells such as hepatoblasts, hepatocytes and/or cholangiocytes, the inducing maturation step comprising generating aggregates of the cell population. Optionally, the method also comprises activating the cAMP pathway within the aggregates and forming co-aggregates.

公开(公告)号：NZ750694A

公开(公告)日：2021-04-30

申请号：NZ75069414

申请日：2014-02-18

Applicant: UNIV HEALTH NETWORK ,  HOSPITAL FOR SICK CHILDREN

Inventor： KELLER GORDON ,  OGAWA SHINICHIRO ,  GHANEKAR ANAND ,  BEAR CHRISTINE ,  KAMATH BINITA M ,  OGAWA MINA ,  SURAPISITCHAT JAMES

IPC: C12N5/071 ,  A61K35/12 ,  A61K35/407 ,  A61P1/16 ,  C12N5/0735 ,  C12Q1/02

Abstract: Methods for producing hepatocyte and/or cholangiocyte lineage cells from pluripotent stem cells, the method comprising (a) specifying the extended nodal agonist treated induced endodermal cell population to obtain a cell population comprising hepatocyte and/or cholangiocyte progenitors by contacting the extended nodal agonist treated induced endodermal cell population with specification media comprising a FGF agonist and a BMP4 agonist and/or active conjugates and/or fragments thereof; (b) inducing maturation, and optionally further lineage specification and/or expansion of the hepatocyte and/or cholangiocyte progenitors of the cell population to obtain a population comprising hepatocyte lineage cells such as hepatoblasts, hepatocytes and/or cholangiocytes, the inducing maturation step comprising generating aggregates of the cell population. Optionally, the method also comprises activating the cAMP pathway within the aggregates and forming co-aggregates.

公开(公告)号：SG10201803131TA

公开(公告)日：2018-06-28

申请号：SG10201803131T

申请日：2014-02-18

Applicant: UNIV HEALTH NETWORK ,  HOSPITAL FOR SICK CHILDREN

Inventor： KELLER GORDON ,  OGAWA SHINICHIRO ,  GHANEKAR ANAND ,  BEAR CHRISTINE ,  KAMATH BINITA M ,  OGAWA MINA ,  SURAPISITCHAT JAMES

Abstract: METHODS FOR GENERATING HEPATOCYTES AND CHOLANGIOCYTES FROM PLURIPOTENT STEM CELLS Methods for producing hepatocyte and/or cholangiocyte lineage cells from pluripotent stem cells, the method comprising (a) specifying the extended nodal agonist treated induced endodermal cell population to obtain a cell population comprising hepatocyte and/or cholangiocyte progenitors by contacting the extended nodal agonist treated induced endodermal cell population with specification media comprising a FGF agonist and a BMP4 agonist and/or active conjugates and/or fragments thereof; (b) inducing maturation, and optionally further lineage specification and/or expansion of the hepatocyte and/or cholangiocyte progenitors of the cell population to obtain a population comprising hepatocyte lineage cells such as hepatoblasts, hepatocytes and/or cholangiocytes, the inducing maturation step comprising generating aggregates of the cell population. Optionally, the method also comprises activating the cAMP pathway within the aggregates and forming co-aggregates . [Fig. 11]

公开(公告)号：AU2014218290A1

公开(公告)日：2015-09-03

申请号：AU2014218290

申请日：2014-02-18

Applicant: HOSPITAL FOR SICK CHILDREN ,  UNIV HEALTH NETWORK

Inventor： KELLER GORDON ,  OGAWA SHINICHIRO ,  GHANEKAR ANAND ,  BEAR CHRISTINE ,  KAMATH BINITA M ,  OGAWA MINA ,  SURAPISITCHAT JAMES

IPC: C12N5/071 ,  A61K35/12 ,  A61K35/407 ,  A61P1/16 ,  C12N5/0735 ,  C12Q1/02

Abstract: Methods for producing hepatocyte and/or cholangiocyte lineage cells from pluripotent stem cells, the method comprising (a) specifying the extended nodal agonist treated induced endodermal cell population to obtain a cell population comprising hepatocyte and/or cholangiocyte progenitors by contacting the extended nodal agonist treated induced endodermal cell population with specification media comprising a FGF agonist and a BMP4 agonist and/or active conjugates and/or fragments thereof; (b) inducing maturation, and optionally further lineage specification and/or expansion of the hepatocyte and/or cholangiocyte progenitors of the cell population to obtain a population comprising hepatocyte lineage cells such as hepatoblasts, hepatocytes and/or cholangiocytes, the inducing maturation step comprising generating aggregates of the cell population. Optionally, the method also comprises activating the cAMP pathway within the aggregates and forming co-aggregates.

公开(公告)号：AU2014218290B2

公开(公告)日：2020-03-12

申请号：AU2014218290

申请日：2014-02-18

Applicant: HOSPITAL FOR SICK CHILDREN ,  UNIV HEALTH NETWORK

Inventor： KELLER GORDON ,  OGAWA SHINICHIRO ,  GHANEKAR ANAND ,  BEAR CHRISTINE ,  KAMATH BINITA M ,  OGAWA MINA ,  SURAPISITCHAT JAMES

IPC: C12N5/071 ,  A61K35/12 ,  A61K35/407 ,  A61P1/16 ,  C12N5/0735 ,  C12Q1/02

Abstract: Methods for producing hepatocyte and/or cholangiocyte lineage cells from pluripotent stem cells, the method comprising (a) specifying the extended nodal agonist treated induced endodermal cell population to obtain a cell population comprising hepatocyte and/or cholangiocyte progenitors by contacting the extended nodal agonist treated induced endodermal cell population with specification media comprising a FGF agonist and a BMP4 agonist and/or active conjugates and/or fragments thereof; (b) inducing maturation, and optionally further lineage specification and/or expansion of the hepatocyte and/or cholangiocyte progenitors of the cell population to obtain a population comprising hepatocyte lineage cells such as hepatoblasts, hepatocytes and/or cholangiocytes, the inducing maturation step comprising generating aggregates of the cell population. Optionally, the method also comprises activating the cAMP pathway within the aggregates and forming co-aggregates.

公开(公告)号：SG11201506520TA

公开(公告)日：2015-09-29

申请号：SG11201506520T

申请日：2014-02-18

Applicant: UNIV HEALTH NETWORK ,  HOSPITAL FOR SICK CHILDREN

Inventor： KELLER GORDON ,  OGAWA SHINICHIRO ,  GHANEKAR ANAND ,  BEAR CHRISTINE ,  KAMATH BINITA M ,  OGAWA MINA ,  SURAPISITCHAT JAMES

IPC: C12N5/071 ,  A61K35/12 ,  A61K35/407 ,  A61P1/16 ,  C12N5/0735 ,  C12Q1/02

Abstract: Methods for producing hepatocyte and/or cholangiocyte lineage cells from pluripotent stem cells, the method comprising (a) specifying the extended nodal agonist treated induced endodermal cell population to obtain a cell population comprising hepatocyte and/or cholangiocyte progenitors by contacting the extended nodal agonist treated induced endodermal cell population with specification media comprising a FGF agonist and a BMP4 agonist and/or active conjugates and/or fragments thereof; (b) inducing maturation, and optionally further lineage specification and/or expansion of the hepatocyte and/or cholangiocyte progenitors of the cell population to obtain a population comprising hepatocyte lineage cells such as hepatoblasts, hepatocytes and/or cholangiocytes, the inducing maturation step comprising generating aggregates of the cell population. Optionally, the method also comprises activating the cAMP pathway within the aggregates and forming co-aggregates.

公开(公告)号：EP2956540A4

公开(公告)日：2016-08-03

申请号：EP14751044

申请日：2014-02-18

Applicant: UNIV HEALTH NETWORK ,  HOSPITAL FOR SICK CHILDREN

Inventor： KELLER GORDON ,  OGAWA SHINICHIRO ,  GHANEKAR ANAND ,  BEAR CHRISTINE ,  KAMATH BINITA M ,  OGAWA MINA ,  SURAPISITCHAT JAMES

IPC: C12N5/071 ,  A61K35/12 ,  A61K35/407 ,  A61P1/16 ,  C12N5/0735 ,  C12Q1/02

CPC classification number: C12N5/067 ,  A61K35/407 ,  C12N5/0679 ,  C12N2501/01 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/12 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/237 ,  C12N2501/415 ,  C12N2501/42 ,  C12N2501/724 ,  C12N2501/727 ,  C12N2501/999 ,  C12N2502/00 ,  C12N2502/14 ,  C12N2503/02 ,  C12N2506/02 ,  C12N2506/45

Abstract: Methods for producing hepatocyte and/or cholangiocyte lineage cells from pluripotent stem cells, the method comprising (a) specifying the extended nodal agonist treated induced endodermal cell population to obtain a cell population comprising hepatocyte and/or cholangiocyte progenitors by contacting the extended nodal agonist treated induced endodermal cell population with specification media comprising a FGF agonist and a BMP4 agonist and/or active conjugates and/or fragments thereof; (b) inducing maturation, and optionally further lineage specification and/or expansion of the hepatocyte and/or cholangiocyte progenitors of the cell population to obtain a population comprising hepatocyte lineage cells such as hepatoblasts, hepatocytes and/or cholangiocytes, the inducing maturation step comprising generating aggregates of the cell population. Optionally, the method also comprises activating the cAMP pathway within the aggregates and forming co-aggregates.