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    GENERATION OF OLIGODENDROGENIC NEURAL PROGENITOR CELLS
    2.
    发明申请
    GENERATION OF OLIGODENDROGENIC NEURAL PROGENITOR CELLS 审中-公开

    公开(公告)号：WO2019023793A1

    公开(公告)日：2019-02-07

    申请号：PCT/CA2018/050926

    申请日：2018-07-30

    Applicant: UNIVERSITY HEALTH NETWORK

    Inventor： FEHLINGS, Michael G. ,  KHAZAEI, Mohamad

    IPC: C12N5/0797 ,  A61K35/30 ,  A61P35/00 ,  A61P25/28

    Abstract: Provided herein are methods of producing, compositions comprising and uses of oligodendrogenic neural progenitor cells (o-NPCs), made using a combination of PDGFR agonist and thyroxin or a thyroxin analogue. The method includes; obtaining ventralized neural progenitor cells (NPCs), the ventralized NPCs expressing Sox2, Nkx6-1, decreased level of Pax6 compared to unpatterned NPCs, and elevated expression of HoxA4 compared to unpatterned NPCs; culturing the ventralized NPCs for about 12 to about 16 days (days 26-40 of Fig. 7; days 12 to 27 of Fig. 10) in neural expansion media (NEM) supplemented with i) PDGFR agonist for the about 12 to about 16 days and ii) thyroxine or a thyroxine analogue for the latter about 7 to about 9 days, to produce o-NPC expressing Sox2 and Nkx2.2, decresed level of Pax6 and Nkx6.1 compared to ventralized NPCs and elevated level of HoxA4 and Olig2 compared to ventralized NPCs.

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